Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease
Aims The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. Methods and Results Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must be added in the case of freeze-drying conidia. Addition of other protectants (Tween-20, peptone, sucrose, glucose, starch and peptone + starch) to skimmed milk before freeze-drying did not improve the conidial viability which was obtained with skimmed milk alone. Glycerol had a negative effect on the lyophilization of E. nigrum conidia. Epicoccum nigrum conidia freeze-dried with skimmed milk, or fluidized bed-dried alone maintained an initial viability for 30 and 90 days, respectively, for storage at room temperature. Epicoccum nigrum conidial viability after spray-drying was lower than 10%. Conclusions The best method to dry E. nigrum conidia was fluidized bed-drying. Conidia without protectants dried by this method had 100% viability and survived for 90 days at room temperature. Significance and Impact of Study This paper deals with methods for the potential formulation of a biocontrol agent which is being tested for eventual commercialization.
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Format: | artículo biblioteca |
Language: | English |
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Wiley
2003
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Subjects: | Biocontrol, Biological agent, Formulation, |
Online Access: | http://hdl.handle.net/20.500.12792/2323 http://hdl.handle.net/10261/290555 |
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dig-inia-es-10261-2905552023-02-17T12:26:52Z Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease Larena Nistal, María Inmaculada De Cal Cortina, Antonieta Liñán, M. Melgarejo, P. Biocontrol Biological agent Formulation Aims The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. Methods and Results Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must be added in the case of freeze-drying conidia. Addition of other protectants (Tween-20, peptone, sucrose, glucose, starch and peptone + starch) to skimmed milk before freeze-drying did not improve the conidial viability which was obtained with skimmed milk alone. Glycerol had a negative effect on the lyophilization of E. nigrum conidia. Epicoccum nigrum conidia freeze-dried with skimmed milk, or fluidized bed-dried alone maintained an initial viability for 30 and 90 days, respectively, for storage at room temperature. Epicoccum nigrum conidial viability after spray-drying was lower than 10%. Conclusions The best method to dry E. nigrum conidia was fluidized bed-drying. Conidia without protectants dried by this method had 100% viability and survived for 90 days at room temperature. Significance and Impact of Study This paper deals with methods for the potential formulation of a biocontrol agent which is being tested for eventual commercialization. 2023-02-17T12:26:52Z 2023-02-17T12:26:52Z 2003 artículo Journal of Applied Microbiology 94(3): 508-514 (2003) 1364-5072 http://hdl.handle.net/20.500.12792/2323 http://hdl.handle.net/10261/290555 10.1046/j.1365-2672.2003.01860.x 1365-2672 en none Wiley |
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Biocontrol Biological agent Formulation Biocontrol Biological agent Formulation Larena Nistal, María Inmaculada De Cal Cortina, Antonieta Liñán, M. Melgarejo, P. Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease |
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Aims The effects of freeze-drying, spray-drying and fluidized bed-drying on survival of Epicoccum nigrum conidia were compared. Methods and Results Viability of E. nigrum conidia (estimated by measuring its germination) was 100% after fluidized bed-drying and freeze-drying, but it was determined that skimmed milk must be added in the case of freeze-drying conidia. Addition of other protectants (Tween-20, peptone, sucrose, glucose, starch and peptone + starch) to skimmed milk before freeze-drying did not improve the conidial viability which was obtained with skimmed milk alone. Glycerol had a negative effect on the lyophilization of E. nigrum conidia. Epicoccum nigrum conidia freeze-dried with skimmed milk, or fluidized bed-dried alone maintained an initial viability for 30 and 90 days, respectively, for storage at room temperature. Epicoccum nigrum conidial viability after spray-drying was lower than 10%. Conclusions The best method to dry E. nigrum conidia was fluidized bed-drying. Conidia without protectants dried by this method had 100% viability and survived for 90 days at room temperature. Significance and Impact of Study This paper deals with methods for the potential formulation of a biocontrol agent which is being tested for eventual commercialization. |
format |
artículo |
topic_facet |
Biocontrol Biological agent Formulation |
author |
Larena Nistal, María Inmaculada De Cal Cortina, Antonieta Liñán, M. Melgarejo, P. |
author_facet |
Larena Nistal, María Inmaculada De Cal Cortina, Antonieta Liñán, M. Melgarejo, P. |
author_sort |
Larena Nistal, María Inmaculada |
title |
Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease |
title_short |
Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease |
title_full |
Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease |
title_fullStr |
Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease |
title_full_unstemmed |
Drying of Epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease |
title_sort |
drying of epicoccum nigrum conidia for obtaining a shelf-stable biological product against brown rot disease |
publisher |
Wiley |
publishDate |
2003 |
url |
http://hdl.handle.net/20.500.12792/2323 http://hdl.handle.net/10261/290555 |
work_keys_str_mv |
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