Influence of staining method on the values of avian sperm head morphometric variables
Contents Computer-assisted systems for the assessment of sperm morphometry (ASMA systems) have been used successfully with several mammalian species. Unfortunately, they have so far been of little use for assessing bird semen, a consequence of the filiform shape of avian spermatozoa. This study compares two staining techniques (Hemacolor® and aniline blue staining) for the morphometric analysis of rooster and red-legged partridge spermatozoa as part of a computer-assisted light microscopy method. For both species, Hemacolor® staining provided a significantly higher percentage of measurable cells (93.7 ± 11.7% in roosters and 71.9 ± 15.3% in red-legged partridges). Hemacolor® also showed greater repeatability (lower coefficients of variation) for length and area in roosters' sperm and for width in the case of red-legged partridge's sperm. In the roosters, the Hemacolor® technique returned significantly (p < 0.05) larger sperm head width and area values than did the aniline blue technique, while the latter resulted in greater sperm head length values (p < 0.05). In the red-legged partridge, no differences were seen in the results for sperm head width and area provided by the two techniques, but aniline blue staining was associated with longer length measurements. In conclusion, the morphometric values recorded differed depending on the staining method and species. However, the Hemacolor® technique might be deemed the more appropriate for computerized sperm assessment systems as it provides larger percentages of measureable cells and shows greater repeatability. © 2015 Blackwell Verlag GmbH.
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2015
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dig-inia-es-10261-2905142023-02-17T12:26:29Z Influence of staining method on the values of avian sperm head morphometric variables Villaverde-Morcillo, S. Esteso Diez, Milagros Cristina Castaño García, Cristina Toledano Díaz, Adolfo López Sebastián, Antonio Campo, J. L. Santiago Moreno, Julián Contents Computer-assisted systems for the assessment of sperm morphometry (ASMA systems) have been used successfully with several mammalian species. Unfortunately, they have so far been of little use for assessing bird semen, a consequence of the filiform shape of avian spermatozoa. This study compares two staining techniques (Hemacolor® and aniline blue staining) for the morphometric analysis of rooster and red-legged partridge spermatozoa as part of a computer-assisted light microscopy method. For both species, Hemacolor® staining provided a significantly higher percentage of measurable cells (93.7 ± 11.7% in roosters and 71.9 ± 15.3% in red-legged partridges). Hemacolor® also showed greater repeatability (lower coefficients of variation) for length and area in roosters' sperm and for width in the case of red-legged partridge's sperm. In the roosters, the Hemacolor® technique returned significantly (p < 0.05) larger sperm head width and area values than did the aniline blue technique, while the latter resulted in greater sperm head length values (p < 0.05). In the red-legged partridge, no differences were seen in the results for sperm head width and area provided by the two techniques, but aniline blue staining was associated with longer length measurements. In conclusion, the morphometric values recorded differed depending on the staining method and species. However, the Hemacolor® technique might be deemed the more appropriate for computerized sperm assessment systems as it provides larger percentages of measureable cells and shows greater repeatability. © 2015 Blackwell Verlag GmbH. 2023-02-17T12:26:29Z 2023-02-17T12:26:29Z 2015 artículo Reproduction in Domestic Animals 50: 750-755 (2015) 0936–6768 http://hdl.handle.net/20.500.12792/4736 http://hdl.handle.net/10261/290514 10.1111/rda.12574 1439-0531 en none Wiley |
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Contents Computer-assisted systems for the assessment of sperm morphometry (ASMA systems) have been used successfully with several mammalian species. Unfortunately, they have so far been of little use for assessing bird semen, a consequence of the filiform shape of avian spermatozoa. This study compares two staining techniques (Hemacolor® and aniline blue staining) for the morphometric analysis of rooster and red-legged partridge spermatozoa as part of a computer-assisted light microscopy method. For both species, Hemacolor® staining provided a significantly higher percentage of measurable cells (93.7 ± 11.7% in roosters and 71.9 ± 15.3% in red-legged partridges). Hemacolor® also showed greater repeatability (lower coefficients of variation) for length and area in roosters' sperm and for width in the case of red-legged partridge's sperm. In the roosters, the Hemacolor® technique returned significantly (p < 0.05) larger sperm head width and area values than did the aniline blue technique, while the latter resulted in greater sperm head length values (p < 0.05). In the red-legged partridge, no differences were seen in the results for sperm head width and area provided by the two techniques, but aniline blue staining was associated with longer length measurements. In conclusion, the morphometric values recorded differed depending on the staining method and species. However, the Hemacolor® technique might be deemed the more appropriate for computerized sperm assessment systems as it provides larger percentages of measureable cells and shows greater repeatability. © 2015 Blackwell Verlag GmbH. |
format |
artículo |
author |
Villaverde-Morcillo, S. Esteso Diez, Milagros Cristina Castaño García, Cristina Toledano Díaz, Adolfo López Sebastián, Antonio Campo, J. L. Santiago Moreno, Julián |
spellingShingle |
Villaverde-Morcillo, S. Esteso Diez, Milagros Cristina Castaño García, Cristina Toledano Díaz, Adolfo López Sebastián, Antonio Campo, J. L. Santiago Moreno, Julián Influence of staining method on the values of avian sperm head morphometric variables |
author_facet |
Villaverde-Morcillo, S. Esteso Diez, Milagros Cristina Castaño García, Cristina Toledano Díaz, Adolfo López Sebastián, Antonio Campo, J. L. Santiago Moreno, Julián |
author_sort |
Villaverde-Morcillo, S. |
title |
Influence of staining method on the values of avian sperm head morphometric variables |
title_short |
Influence of staining method on the values of avian sperm head morphometric variables |
title_full |
Influence of staining method on the values of avian sperm head morphometric variables |
title_fullStr |
Influence of staining method on the values of avian sperm head morphometric variables |
title_full_unstemmed |
Influence of staining method on the values of avian sperm head morphometric variables |
title_sort |
influence of staining method on the values of avian sperm head morphometric variables |
publisher |
Wiley |
publishDate |
2015 |
url |
http://hdl.handle.net/20.500.12792/4736 http://hdl.handle.net/10261/290514 |
work_keys_str_mv |
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