The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV)
The aim of this work was to improve the knowledge about the factors contributing to the immunogenicity of the DNA vaccines based on the viral haemorrhagic septicaemia virus glycoprotein G gene, through identifying the rainbow trout Toll-like receptor 9 (Omtlr9) gene that curiously contains an insertion of an incomplete transposon at the 5′-end of the third intron. Concerning the role played by this receptor in the fish innate defence, in response to the injection of a plasmid (pAE6) encoding or not the viral haemorrhagic septicaemia rhabdovirus (VHSV) glycoprotein G gene (pAE6-G), the presence of Omtlr9 transcripts remained unchanged in the fish secondary lymphoid organs while was highly increased at the injection site (muscle). The level of Omtlr9 transcripts correlated with those of cluster of differentiation 83 (cd83) and CXC chemokine receptor 4 (cxcr4), suggesting the recruitment of dendritic-like cells into the muscle as the source of Omtlr9 expressing cells. Transcription of tumour necrosis factor-alpha (tnfα) and interleukin-6 (il6) genes, two cytokines directly related to TLR9 induction with unmethylated CpG oligodeoxynucleotides (CpG ODNs), was solely observed in head kidney and spleen of the fish immunised with pAE6-G. Thus, the glycoprotein G of VHSV could be more implicated in triggering the pathways for TNF-α and IL6 production than the recognition of the unmethylated CpG motifs of the plasmid backbone by OmTLR9. Therefore, our results seem to indicate that OmTLR9-mediated recognition of plasmid DNA is not the key of the innate immune recognition of the adjuvant elements of fish DNA vaccines. © 2009 Elsevier Ltd. All rights reserved.
Main Authors: | , , , , , |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2009
|
Subjects: | Innate immunity, Toll-like receptor, DNA vaccine, Viral haemorrhagic septicaemia rhabdovirus, Rainbow trout, Tumour necrosis factor, Interleukin, Unmethylated CpG motifs, CXC chemokine receptor, Cluster of differentiation 83, |
Online Access: | http://hdl.handle.net/20.500.12792/3228 http://hdl.handle.net/10261/290435 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
id |
dig-inia-es-10261-290435 |
---|---|
record_format |
koha |
spelling |
dig-inia-es-10261-2904352023-02-17T12:25:36Z The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV) Ortega-Villaizan, M. Chico, V. Falco, A. Perez, L. Coll, J. M. Estepa, A. Innate immunity Toll-like receptor DNA vaccine Viral haemorrhagic septicaemia rhabdovirus Rainbow trout Tumour necrosis factor Interleukin Unmethylated CpG motifs CXC chemokine receptor Cluster of differentiation 83 The aim of this work was to improve the knowledge about the factors contributing to the immunogenicity of the DNA vaccines based on the viral haemorrhagic septicaemia virus glycoprotein G gene, through identifying the rainbow trout Toll-like receptor 9 (Omtlr9) gene that curiously contains an insertion of an incomplete transposon at the 5′-end of the third intron. Concerning the role played by this receptor in the fish innate defence, in response to the injection of a plasmid (pAE6) encoding or not the viral haemorrhagic septicaemia rhabdovirus (VHSV) glycoprotein G gene (pAE6-G), the presence of Omtlr9 transcripts remained unchanged in the fish secondary lymphoid organs while was highly increased at the injection site (muscle). The level of Omtlr9 transcripts correlated with those of cluster of differentiation 83 (cd83) and CXC chemokine receptor 4 (cxcr4), suggesting the recruitment of dendritic-like cells into the muscle as the source of Omtlr9 expressing cells. Transcription of tumour necrosis factor-alpha (tnfα) and interleukin-6 (il6) genes, two cytokines directly related to TLR9 induction with unmethylated CpG oligodeoxynucleotides (CpG ODNs), was solely observed in head kidney and spleen of the fish immunised with pAE6-G. Thus, the glycoprotein G of VHSV could be more implicated in triggering the pathways for TNF-α and IL6 production than the recognition of the unmethylated CpG motifs of the plasmid backbone by OmTLR9. Therefore, our results seem to indicate that OmTLR9-mediated recognition of plasmid DNA is not the key of the innate immune recognition of the adjuvant elements of fish DNA vaccines. © 2009 Elsevier Ltd. All rights reserved. 2023-02-17T12:25:36Z 2023-02-17T12:25:36Z 2009 artículo Molecular Immunology 46(8-9): 1710-1717 (2009) 0161-5890 http://hdl.handle.net/20.500.12792/3228 http://hdl.handle.net/10261/290435 10.1016/j.molimm.2009.02.006 en none Elsevier |
institution |
INIA ES |
collection |
DSpace |
country |
España |
countrycode |
ES |
component |
Bibliográfico |
access |
En linea |
databasecode |
dig-inia-es |
tag |
biblioteca |
region |
Europa del Sur |
libraryname |
Biblioteca del INIA España |
language |
English |
topic |
Innate immunity Toll-like receptor DNA vaccine Viral haemorrhagic septicaemia rhabdovirus Rainbow trout Tumour necrosis factor Interleukin Unmethylated CpG motifs CXC chemokine receptor Cluster of differentiation 83 Innate immunity Toll-like receptor DNA vaccine Viral haemorrhagic septicaemia rhabdovirus Rainbow trout Tumour necrosis factor Interleukin Unmethylated CpG motifs CXC chemokine receptor Cluster of differentiation 83 |
spellingShingle |
Innate immunity Toll-like receptor DNA vaccine Viral haemorrhagic septicaemia rhabdovirus Rainbow trout Tumour necrosis factor Interleukin Unmethylated CpG motifs CXC chemokine receptor Cluster of differentiation 83 Innate immunity Toll-like receptor DNA vaccine Viral haemorrhagic septicaemia rhabdovirus Rainbow trout Tumour necrosis factor Interleukin Unmethylated CpG motifs CXC chemokine receptor Cluster of differentiation 83 Ortega-Villaizan, M. Chico, V. Falco, A. Perez, L. Coll, J. M. Estepa, A. The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV) |
description |
The aim of this work was to improve the knowledge about the factors contributing to the immunogenicity of the DNA vaccines based on the viral haemorrhagic septicaemia virus glycoprotein G gene, through identifying the rainbow trout Toll-like receptor 9 (Omtlr9) gene that curiously contains an insertion of an incomplete transposon at the 5′-end of the third intron. Concerning the role played by this receptor in the fish innate defence, in response to the injection of a plasmid (pAE6) encoding or not the viral haemorrhagic septicaemia rhabdovirus (VHSV) glycoprotein G gene (pAE6-G), the presence of Omtlr9 transcripts remained unchanged in the fish secondary lymphoid organs while was highly increased at the injection site (muscle). The level of Omtlr9 transcripts correlated with those of cluster of differentiation 83 (cd83) and CXC chemokine receptor 4 (cxcr4), suggesting the recruitment of dendritic-like cells into the muscle as the source of Omtlr9 expressing cells. Transcription of tumour necrosis factor-alpha (tnfα) and interleukin-6 (il6) genes, two cytokines directly related to TLR9 induction with unmethylated CpG oligodeoxynucleotides (CpG ODNs), was solely observed in head kidney and spleen of the fish immunised with pAE6-G. Thus, the glycoprotein G of VHSV could be more implicated in triggering the pathways for TNF-α and IL6 production than the recognition of the unmethylated CpG motifs of the plasmid backbone by OmTLR9. Therefore, our results seem to indicate that OmTLR9-mediated recognition of plasmid DNA is not the key of the innate immune recognition of the adjuvant elements of fish DNA vaccines. © 2009 Elsevier Ltd. All rights reserved. |
format |
artículo |
topic_facet |
Innate immunity Toll-like receptor DNA vaccine Viral haemorrhagic septicaemia rhabdovirus Rainbow trout Tumour necrosis factor Interleukin Unmethylated CpG motifs CXC chemokine receptor Cluster of differentiation 83 |
author |
Ortega-Villaizan, M. Chico, V. Falco, A. Perez, L. Coll, J. M. Estepa, A. |
author_facet |
Ortega-Villaizan, M. Chico, V. Falco, A. Perez, L. Coll, J. M. Estepa, A. |
author_sort |
Ortega-Villaizan, M. |
title |
The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV) |
title_short |
The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV) |
title_full |
The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV) |
title_fullStr |
The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV) |
title_full_unstemmed |
The rainbow trout TLR9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein G of the viral haemorrhagic septicaemia rhabdovirus (VHSV) |
title_sort |
rainbow trout tlr9 gene and its role in the immune responses elicited by a plasmid encoding the glycoprotein g of the viral haemorrhagic septicaemia rhabdovirus (vhsv) |
publisher |
Elsevier |
publishDate |
2009 |
url |
http://hdl.handle.net/20.500.12792/3228 http://hdl.handle.net/10261/290435 |
work_keys_str_mv |
AT ortegavillaizanm therainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT chicov therainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT falcoa therainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT perezl therainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT colljm therainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT estepaa therainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT ortegavillaizanm rainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT chicov rainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT falcoa rainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT perezl rainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT colljm rainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv AT estepaa rainbowtrouttlr9geneanditsroleintheimmuneresponseselicitedbyaplasmidencodingtheglycoproteingoftheviralhaemorrhagicsepticaemiarhabdovirusvhsv |
_version_ |
1767603085527482368 |