Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells
Antibody-mediated targeting of antigen to specific antigen presenting cells (APC) receptors is an attractive strategy to enhance T cell immune responses to weak immunogenic antigens. Here, we describe the characterization of two monoclonal antibodies (mAb) against different epitopes of porcine sialoadhesin (Sn) and evaluate in vitro the potential of targeting this receptor for delivery of antigens to APC for T cell stimulation. The specificity of these mAb was determined by amino acid sequence analysis of peptides derived from the affinity purified antigen. Porcine Sn is expressed by macrophages present in the border between white and red pulp of the spleen and in the subcapsular sinus of lymph nodes, an appropriate location for trapping blood and lymph-borne antigens. It is also expressed by alveolar macrophages and monocyte-derived dendritic cells (MoDC). Blood monocytes are negative for this molecule, but its expression can be induced by treatment with IFN-andalpha;. MAb bound to Sn is rapidly endocytosed. MAb to sialoadhesin induced in vitro T cell proliferation at concentrations 100-fold lower than the non-targeting control mAb when using T lymphocytes from pigs immunized with mouse immunoglobulins as responder cells and IFN-andalpha; treated monocytes or MoDC as APC, suggesting a role of sialoadhesin in antigen uptake and/or delivery into the presentation pathway in APC. © 2009 INRA EDP Sciences.
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Format: | artículo biblioteca |
Language: | English |
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BioMed Central
2009
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Subjects: | Sialoadhesin, Monoclonal antibody, Macrophage, Antigen-targeting, Swine, |
Online Access: | http://hdl.handle.net/20.500.12792/3697 http://hdl.handle.net/10261/290278 |
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dig-inia-es-10261-2902782023-02-17T08:29:14Z Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells Revilla Calvo, Concepción Poderoso, T. Martínez, P. Álvarez, B. López-Fuertes, L. Alonso, F. Ezquerra Martínez, Ángel Domínguez, J. Sialoadhesin Monoclonal antibody Macrophage Antigen-targeting Swine Antibody-mediated targeting of antigen to specific antigen presenting cells (APC) receptors is an attractive strategy to enhance T cell immune responses to weak immunogenic antigens. Here, we describe the characterization of two monoclonal antibodies (mAb) against different epitopes of porcine sialoadhesin (Sn) and evaluate in vitro the potential of targeting this receptor for delivery of antigens to APC for T cell stimulation. The specificity of these mAb was determined by amino acid sequence analysis of peptides derived from the affinity purified antigen. Porcine Sn is expressed by macrophages present in the border between white and red pulp of the spleen and in the subcapsular sinus of lymph nodes, an appropriate location for trapping blood and lymph-borne antigens. It is also expressed by alveolar macrophages and monocyte-derived dendritic cells (MoDC). Blood monocytes are negative for this molecule, but its expression can be induced by treatment with IFN-andalpha;. MAb bound to Sn is rapidly endocytosed. MAb to sialoadhesin induced in vitro T cell proliferation at concentrations 100-fold lower than the non-targeting control mAb when using T lymphocytes from pigs immunized with mouse immunoglobulins as responder cells and IFN-andalpha; treated monocytes or MoDC as APC, suggesting a role of sialoadhesin in antigen uptake and/or delivery into the presentation pathway in APC. © 2009 INRA EDP Sciences. 2023-02-17T08:29:14Z 2023-02-17T08:29:14Z 2009 artículo Veterinary Research 40(3): e14 (2009) 0928-4249 http://hdl.handle.net/20.500.12792/3697 http://hdl.handle.net/10261/290278 10.1051/vetres2008052 1297-9716 en open BioMed Central |
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Sialoadhesin Monoclonal antibody Macrophage Antigen-targeting Swine Sialoadhesin Monoclonal antibody Macrophage Antigen-targeting Swine |
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Sialoadhesin Monoclonal antibody Macrophage Antigen-targeting Swine Sialoadhesin Monoclonal antibody Macrophage Antigen-targeting Swine Revilla Calvo, Concepción Poderoso, T. Martínez, P. Álvarez, B. López-Fuertes, L. Alonso, F. Ezquerra Martínez, Ángel Domínguez, J. Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells |
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Antibody-mediated targeting of antigen to specific antigen presenting cells (APC) receptors is an attractive strategy to enhance T cell immune responses to weak immunogenic antigens. Here, we describe the characterization of two monoclonal antibodies (mAb) against different epitopes of porcine sialoadhesin (Sn) and evaluate in vitro the potential of targeting this receptor for delivery of antigens to APC for T cell stimulation. The specificity of these mAb was determined by amino acid sequence analysis of peptides derived from the affinity purified antigen. Porcine Sn is expressed by macrophages present in the border between white and red pulp of the spleen and in the subcapsular sinus of lymph nodes, an appropriate location for trapping blood and lymph-borne antigens. It is also expressed by alveolar macrophages and monocyte-derived dendritic cells (MoDC). Blood monocytes are negative for this molecule, but its expression can be induced by treatment with IFN-andalpha;. MAb bound to Sn is rapidly endocytosed. MAb to sialoadhesin induced in vitro T cell proliferation at concentrations 100-fold lower than the non-targeting control mAb when using T lymphocytes from pigs immunized with mouse immunoglobulins as responder cells and IFN-andalpha; treated monocytes or MoDC as APC, suggesting a role of sialoadhesin in antigen uptake and/or delivery into the presentation pathway in APC. © 2009 INRA EDP Sciences. |
format |
artículo |
topic_facet |
Sialoadhesin Monoclonal antibody Macrophage Antigen-targeting Swine |
author |
Revilla Calvo, Concepción Poderoso, T. Martínez, P. Álvarez, B. López-Fuertes, L. Alonso, F. Ezquerra Martínez, Ángel Domínguez, J. |
author_facet |
Revilla Calvo, Concepción Poderoso, T. Martínez, P. Álvarez, B. López-Fuertes, L. Alonso, F. Ezquerra Martínez, Ángel Domínguez, J. |
author_sort |
Revilla Calvo, Concepción |
title |
Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells |
title_short |
Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells |
title_full |
Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells |
title_fullStr |
Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells |
title_full_unstemmed |
Targeting to porcine sialoadhesin receptor receptor improves antigen presentation to T cells |
title_sort |
targeting to porcine sialoadhesin receptor receptor improves antigen presentation to t cells |
publisher |
BioMed Central |
publishDate |
2009 |
url |
http://hdl.handle.net/20.500.12792/3697 http://hdl.handle.net/10261/290278 |
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