Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry
We have developed a three-colour flow cytometric assay for phenotypic characterization of porcine IFN-γ-producing lymphocytes. Analyses of activated swine peripheral blood mononuclear cells (PBMC) showed a significant difference in the proportion of IFN-γ producing cells between young and adult animals (13.2 ± 5.8% versus 34.2 ± 5.7%). The majority of IFN-γ producing cells were αβ T lymphocytes, although there was also an important proportion of γδ T cells particularly in young animals. Within the αβ T lymphocytes, the double positive CD4+CD8lo subset, that contains memory T cells, produced high levels of IFN-γ, whereas the CD8hi T cells ranged from low to high levels of IFN-γ. Also, consistent with a higher production by memory T cells, the CD45RA- subset of both CD4+ and CD8+ cells contained higher numbers of IFN-γ producing cells than the CD45RA+ subset. Finally, no production of IFN-γ by either B cells (CD21+) or monocytes (SWC3+) was detected. This assay may be useful for the assessment of cell-mediated immunity in vaccine trials and may contribute to our understanding of the role of IFN-γ in protective immunity against important viral diseases of the pig. © 2002 Elsevier Science B.V. All rights reserved.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2002
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Subjects: | Flow cytometry, Intracellular staining, Lymphocyte subsets, Monoclonal antibodies, Porcine IFN-y, |
Online Access: | http://hdl.handle.net/20.500.12792/3828 http://hdl.handle.net/10261/290230 |
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dig-inia-es-10261-2902302023-02-17T08:28:45Z Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry Rodríguez-Carreño, M. P. López-Fuertes, L. Revilla Calvo, Concepción Ezquerra Martínez, Ángel Alonso, F. Domínguez, J. Flow cytometry Intracellular staining Lymphocyte subsets Monoclonal antibodies Porcine IFN-y We have developed a three-colour flow cytometric assay for phenotypic characterization of porcine IFN-γ-producing lymphocytes. Analyses of activated swine peripheral blood mononuclear cells (PBMC) showed a significant difference in the proportion of IFN-γ producing cells between young and adult animals (13.2 ± 5.8% versus 34.2 ± 5.7%). The majority of IFN-γ producing cells were αβ T lymphocytes, although there was also an important proportion of γδ T cells particularly in young animals. Within the αβ T lymphocytes, the double positive CD4+CD8lo subset, that contains memory T cells, produced high levels of IFN-γ, whereas the CD8hi T cells ranged from low to high levels of IFN-γ. Also, consistent with a higher production by memory T cells, the CD45RA- subset of both CD4+ and CD8+ cells contained higher numbers of IFN-γ producing cells than the CD45RA+ subset. Finally, no production of IFN-γ by either B cells (CD21+) or monocytes (SWC3+) was detected. This assay may be useful for the assessment of cell-mediated immunity in vaccine trials and may contribute to our understanding of the role of IFN-γ in protective immunity against important viral diseases of the pig. © 2002 Elsevier Science B.V. All rights reserved. 2023-02-17T08:28:45Z 2023-02-17T08:28:45Z 2002 artículo Journal of Immunological Methods 259(1-2): 171-179 (2002) 0022-1759 http://hdl.handle.net/20.500.12792/3828 http://hdl.handle.net/10261/290230 10.1016/S0022-1759(01)00511-7 en none Elsevier |
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Flow cytometry Intracellular staining Lymphocyte subsets Monoclonal antibodies Porcine IFN-y Flow cytometry Intracellular staining Lymphocyte subsets Monoclonal antibodies Porcine IFN-y |
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Flow cytometry Intracellular staining Lymphocyte subsets Monoclonal antibodies Porcine IFN-y Flow cytometry Intracellular staining Lymphocyte subsets Monoclonal antibodies Porcine IFN-y Rodríguez-Carreño, M. P. López-Fuertes, L. Revilla Calvo, Concepción Ezquerra Martínez, Ángel Alonso, F. Domínguez, J. Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry |
description |
We have developed a three-colour flow cytometric assay for phenotypic characterization of porcine IFN-γ-producing lymphocytes. Analyses of activated swine peripheral blood mononuclear cells (PBMC) showed a significant difference in the proportion of IFN-γ producing cells between young and adult animals (13.2 ± 5.8% versus 34.2 ± 5.7%). The majority of IFN-γ producing cells were αβ T lymphocytes, although there was also an important proportion of γδ T cells particularly in young animals. Within the αβ T lymphocytes, the double positive CD4+CD8lo subset, that contains memory T cells, produced high levels of IFN-γ, whereas the CD8hi T cells ranged from low to high levels of IFN-γ. Also, consistent with a higher production by memory T cells, the CD45RA- subset of both CD4+ and CD8+ cells contained higher numbers of IFN-γ producing cells than the CD45RA+ subset. Finally, no production of IFN-γ by either B cells (CD21+) or monocytes (SWC3+) was detected. This assay may be useful for the assessment of cell-mediated immunity in vaccine trials and may contribute to our understanding of the role of IFN-γ in protective immunity against important viral diseases of the pig. © 2002 Elsevier Science B.V. All rights reserved. |
format |
artículo |
topic_facet |
Flow cytometry Intracellular staining Lymphocyte subsets Monoclonal antibodies Porcine IFN-y |
author |
Rodríguez-Carreño, M. P. López-Fuertes, L. Revilla Calvo, Concepción Ezquerra Martínez, Ángel Alonso, F. Domínguez, J. |
author_facet |
Rodríguez-Carreño, M. P. López-Fuertes, L. Revilla Calvo, Concepción Ezquerra Martínez, Ángel Alonso, F. Domínguez, J. |
author_sort |
Rodríguez-Carreño, M. P. |
title |
Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry |
title_short |
Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry |
title_full |
Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry |
title_fullStr |
Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry |
title_full_unstemmed |
Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry |
title_sort |
phenotypic characterization of porcine ifn-γ-producing lymphocytes by flow cytometry |
publisher |
Elsevier |
publishDate |
2002 |
url |
http://hdl.handle.net/20.500.12792/3828 http://hdl.handle.net/10261/290230 |
work_keys_str_mv |
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