Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry

We have developed a three-colour flow cytometric assay for phenotypic characterization of porcine IFN-γ-producing lymphocytes. Analyses of activated swine peripheral blood mononuclear cells (PBMC) showed a significant difference in the proportion of IFN-γ producing cells between young and adult animals (13.2 ± 5.8% versus 34.2 ± 5.7%). The majority of IFN-γ producing cells were αβ T lymphocytes, although there was also an important proportion of γδ T cells particularly in young animals. Within the αβ T lymphocytes, the double positive CD4+CD8lo subset, that contains memory T cells, produced high levels of IFN-γ, whereas the CD8hi T cells ranged from low to high levels of IFN-γ. Also, consistent with a higher production by memory T cells, the CD45RA- subset of both CD4+ and CD8+ cells contained higher numbers of IFN-γ producing cells than the CD45RA+ subset. Finally, no production of IFN-γ by either B cells (CD21+) or monocytes (SWC3+) was detected. This assay may be useful for the assessment of cell-mediated immunity in vaccine trials and may contribute to our understanding of the role of IFN-γ in protective immunity against important viral diseases of the pig. © 2002 Elsevier Science B.V. All rights reserved.

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Bibliographic Details
Main Authors: Rodríguez-Carreño, M. P., López-Fuertes, L., Revilla Calvo, Concepción, Ezquerra Martínez, Ángel, Alonso, F., Domínguez, J.
Format: artículo biblioteca
Language:English
Published: Elsevier 2002
Subjects:Flow cytometry, Intracellular staining, Lymphocyte subsets, Monoclonal antibodies, Porcine IFN-y,
Online Access:http://hdl.handle.net/20.500.12792/3828
http://hdl.handle.net/10261/290230
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spelling dig-inia-es-10261-2902302023-02-17T08:28:45Z Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry Rodríguez-Carreño, M. P. López-Fuertes, L. Revilla Calvo, Concepción Ezquerra Martínez, Ángel Alonso, F. Domínguez, J. Flow cytometry Intracellular staining Lymphocyte subsets Monoclonal antibodies Porcine IFN-y We have developed a three-colour flow cytometric assay for phenotypic characterization of porcine IFN-γ-producing lymphocytes. Analyses of activated swine peripheral blood mononuclear cells (PBMC) showed a significant difference in the proportion of IFN-γ producing cells between young and adult animals (13.2 ± 5.8% versus 34.2 ± 5.7%). The majority of IFN-γ producing cells were αβ T lymphocytes, although there was also an important proportion of γδ T cells particularly in young animals. Within the αβ T lymphocytes, the double positive CD4+CD8lo subset, that contains memory T cells, produced high levels of IFN-γ, whereas the CD8hi T cells ranged from low to high levels of IFN-γ. Also, consistent with a higher production by memory T cells, the CD45RA- subset of both CD4+ and CD8+ cells contained higher numbers of IFN-γ producing cells than the CD45RA+ subset. Finally, no production of IFN-γ by either B cells (CD21+) or monocytes (SWC3+) was detected. This assay may be useful for the assessment of cell-mediated immunity in vaccine trials and may contribute to our understanding of the role of IFN-γ in protective immunity against important viral diseases of the pig. © 2002 Elsevier Science B.V. All rights reserved. 2023-02-17T08:28:45Z 2023-02-17T08:28:45Z 2002 artículo Journal of Immunological Methods 259(1-2): 171-179 (2002) 0022-1759 http://hdl.handle.net/20.500.12792/3828 http://hdl.handle.net/10261/290230 10.1016/S0022-1759(01)00511-7 en none Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Flow cytometry
Intracellular staining
Lymphocyte subsets
Monoclonal antibodies
Porcine IFN-y
Flow cytometry
Intracellular staining
Lymphocyte subsets
Monoclonal antibodies
Porcine IFN-y
spellingShingle Flow cytometry
Intracellular staining
Lymphocyte subsets
Monoclonal antibodies
Porcine IFN-y
Flow cytometry
Intracellular staining
Lymphocyte subsets
Monoclonal antibodies
Porcine IFN-y
Rodríguez-Carreño, M. P.
López-Fuertes, L.
Revilla Calvo, Concepción
Ezquerra Martínez, Ángel
Alonso, F.
Domínguez, J.
Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry
description We have developed a three-colour flow cytometric assay for phenotypic characterization of porcine IFN-γ-producing lymphocytes. Analyses of activated swine peripheral blood mononuclear cells (PBMC) showed a significant difference in the proportion of IFN-γ producing cells between young and adult animals (13.2 ± 5.8% versus 34.2 ± 5.7%). The majority of IFN-γ producing cells were αβ T lymphocytes, although there was also an important proportion of γδ T cells particularly in young animals. Within the αβ T lymphocytes, the double positive CD4+CD8lo subset, that contains memory T cells, produced high levels of IFN-γ, whereas the CD8hi T cells ranged from low to high levels of IFN-γ. Also, consistent with a higher production by memory T cells, the CD45RA- subset of both CD4+ and CD8+ cells contained higher numbers of IFN-γ producing cells than the CD45RA+ subset. Finally, no production of IFN-γ by either B cells (CD21+) or monocytes (SWC3+) was detected. This assay may be useful for the assessment of cell-mediated immunity in vaccine trials and may contribute to our understanding of the role of IFN-γ in protective immunity against important viral diseases of the pig. © 2002 Elsevier Science B.V. All rights reserved.
format artículo
topic_facet Flow cytometry
Intracellular staining
Lymphocyte subsets
Monoclonal antibodies
Porcine IFN-y
author Rodríguez-Carreño, M. P.
López-Fuertes, L.
Revilla Calvo, Concepción
Ezquerra Martínez, Ángel
Alonso, F.
Domínguez, J.
author_facet Rodríguez-Carreño, M. P.
López-Fuertes, L.
Revilla Calvo, Concepción
Ezquerra Martínez, Ángel
Alonso, F.
Domínguez, J.
author_sort Rodríguez-Carreño, M. P.
title Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry
title_short Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry
title_full Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry
title_fullStr Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry
title_full_unstemmed Phenotypic characterization of porcine IFN-γ-producing lymphocytes by flow cytometry
title_sort phenotypic characterization of porcine ifn-γ-producing lymphocytes by flow cytometry
publisher Elsevier
publishDate 2002
url http://hdl.handle.net/20.500.12792/3828
http://hdl.handle.net/10261/290230
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