Male fertility is reduced by chronic intermittent hypoxia mimicking sleep apnea in mice

Study Objectives Obstructive sleep apnea (OSA) is characterized by intermittent hypoxia and oxidative stress. However, it is unknown whether intermittent hypoxia mimicking OSA modifies male fertility. We tested the hypothesis that male fertility is reduced by chronic intermittent hypoxia mimicking OSA in a mouse model. Design Case-control comparison in a murine model. Setting University research laboratory. Participants Eighteen F1 (C57BL/6xCBA) male mice. Interventions Mice were subjected to a pattern of periodic hypoxia (20 sec at 5% O2 followed by 40 sec of room air) 6 h/day for 60 days or normoxia. After this period, mice performed a mating trial to determine effective fertility by assessing the number of pregnant females and fetuses. Measurements and Results After euthanasia, oxidative stress in testes was assessed by measuring the expression of glutathione peroxidase 1 (Gpx1) and superoxide dismutase-1 (Sod1) by reverse-transcription polymerase chain reaction. Sperm motility was determined by Integrated Semen Analysis System (ISAS). Intermittent hypoxia significantly increased testicular oxidative stress, showing a reduction in the expression of Gpx1 and Sod1 by 38.9% and 34.4%, respectively, as compared with normoxia (P < 0.05). Progressive sperm motility was significantly reduced from 27.0 ± 6.4% in normoxia to 12.8 ± 1.8% in the intermittent hypoxia group (P = 0.04). The proportion of pregnant females and number of fetuses per mating was significantly lower in the intermittent hypoxia group (0.33 ± 0.10 and 2.45 ± 0.73, respectively) than in normoxic controls (0.72 ± 0.16 and 5.80 ± 1.24, respectively). Conclusions These results suggest that the intermittent hypoxia associated with obstructive sleep apnea (OSA) could induce fertility reduction in male patients with this sleep breathing disorder.

Bibliographic Details

Main Authors:	Torres, M., Laguna-Barraza, R., Dalmases, M., Calle, A., Pericuesta Camacho, Eva, Montserrat, J. M., Navajas, D., Gutiérrez Adán, Alfonso, Farré, R.
Format:	artículo biblioteca
Language:	English
Published:	Oxford University Press 2014
Subjects:	Obstructive sleep apnea, Hypoxia, Male fertility, Oxidative stress,
Online Access:	http://hdl.handle.net/20.500.12792/4540 http://hdl.handle.net/10261/290068
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