Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations
This work identified the presence of AQPs in frozen-thawed sperm of wild ruminants and assessed the influence of the interaction between photoperiod and thyroxine on AQP expression, and on testosterone secretion. Thyroxine and melatonin were administered to ibexes. In a second experiment, performed in mouflons, circulating thyroxine was reduced via treatment with propylth iouracil (PTU), and an artificial long day (LD) photoperiod established. In the ibexes, the melatonin treatment increased the blood plasma testosterone concentration, reduced the cryoresistance ratio (CR) for sperm viability and the presence of an intact acrosome, and increased the percentage of sperm with AQP7 in the acrosome and of AQP3 and AQP10 in the midpiece. In the mouflons, neither the PTU treatment, the LD, nor the combination of both affected the CR of any sperm variable. The percentage of sperm with AQP3 increased in the post-acrosome region but decreased in the tail in the LD+PTU group. The percentage of sperm with AQP10 in the principal piece and endpiece was lower in the PTU+LD group than in the control and LD groups. The influence of photoperiod/melatonin on AQP expression might be indirectly exerted through changes in the testosterone concentration, and thus ultimately affect sperm cryoresistance.
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Format: | artículo biblioteca |
Language: | English |
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Multidisciplinary Digital Publishing Institute
2022-03-03
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Subjects: | Iberian ibex, Mouflon, Aquaglyceroporins, Melatonin, Sperm cryoresistance, Thyroxine, |
Online Access: | http://hdl.handle.net/20.500.12792/6188 http://hdl.handle.net/10261/289650 |
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dig-inia-es-10261-2896502023-02-16T14:55:08Z Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations Santiago-Moreno, Julián Pequeño, Belén Martinez-Madrid, Belen Castaño, Cristina Bóveda, Paula Velázquez, Rosario Toledano-Díaz, Adolfo Álvarez-Rodríguez, Manuel Rodríguez-Martínez, Heriberto Iberian ibex Mouflon Aquaglyceroporins Melatonin Sperm cryoresistance Thyroxine This work identified the presence of AQPs in frozen-thawed sperm of wild ruminants and assessed the influence of the interaction between photoperiod and thyroxine on AQP expression, and on testosterone secretion. Thyroxine and melatonin were administered to ibexes. In a second experiment, performed in mouflons, circulating thyroxine was reduced via treatment with propylth iouracil (PTU), and an artificial long day (LD) photoperiod established. In the ibexes, the melatonin treatment increased the blood plasma testosterone concentration, reduced the cryoresistance ratio (CR) for sperm viability and the presence of an intact acrosome, and increased the percentage of sperm with AQP7 in the acrosome and of AQP3 and AQP10 in the midpiece. In the mouflons, neither the PTU treatment, the LD, nor the combination of both affected the CR of any sperm variable. The percentage of sperm with AQP3 increased in the post-acrosome region but decreased in the tail in the LD+PTU group. The percentage of sperm with AQP10 in the principal piece and endpiece was lower in the PTU+LD group than in the control and LD groups. The influence of photoperiod/melatonin on AQP expression might be indirectly exerted through changes in the testosterone concentration, and thus ultimately affect sperm cryoresistance. 2023-02-16T12:22:35Z 2023-02-16T12:22:35Z 2022-03-03 artículo International Journal of Molecular Sciences 23 (6) 2903 (2022) 1661-6596 http://hdl.handle.net/20.500.12792/6188 http://hdl.handle.net/10261/289650 10.3390/ijms23062903 1422-0067 en open Multidisciplinary Digital Publishing Institute |
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Iberian ibex Mouflon Aquaglyceroporins Melatonin Sperm cryoresistance Thyroxine Iberian ibex Mouflon Aquaglyceroporins Melatonin Sperm cryoresistance Thyroxine |
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Iberian ibex Mouflon Aquaglyceroporins Melatonin Sperm cryoresistance Thyroxine Iberian ibex Mouflon Aquaglyceroporins Melatonin Sperm cryoresistance Thyroxine Santiago-Moreno, Julián Pequeño, Belén Martinez-Madrid, Belen Castaño, Cristina Bóveda, Paula Velázquez, Rosario Toledano-Díaz, Adolfo Álvarez-Rodríguez, Manuel Rodríguez-Martínez, Heriberto Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations |
description |
This work identified the presence of AQPs in frozen-thawed sperm of wild ruminants and
assessed the influence of the interaction between photoperiod and thyroxine on AQP expression,
and on testosterone secretion. Thyroxine and melatonin were administered to ibexes. In a second
experiment, performed in mouflons, circulating thyroxine was reduced via treatment with propylth iouracil (PTU), and an artificial long day (LD) photoperiod established. In the ibexes, the melatonin
treatment increased the blood plasma testosterone concentration, reduced the cryoresistance ratio
(CR) for sperm viability and the presence of an intact acrosome, and increased the percentage of
sperm with AQP7 in the acrosome and of AQP3 and AQP10 in the midpiece. In the mouflons, neither
the PTU treatment, the LD, nor the combination of both affected the CR of any sperm variable. The
percentage of sperm with AQP3 increased in the post-acrosome region but decreased in the tail in the
LD+PTU group. The percentage of sperm with AQP10 in the principal piece and endpiece was lower
in the PTU+LD group than in the control and LD groups. The influence of photoperiod/melatonin
on AQP expression might be indirectly exerted through changes in the testosterone concentration,
and thus ultimately affect sperm cryoresistance. |
format |
artículo |
topic_facet |
Iberian ibex Mouflon Aquaglyceroporins Melatonin Sperm cryoresistance Thyroxine |
author |
Santiago-Moreno, Julián Pequeño, Belén Martinez-Madrid, Belen Castaño, Cristina Bóveda, Paula Velázquez, Rosario Toledano-Díaz, Adolfo Álvarez-Rodríguez, Manuel Rodríguez-Martínez, Heriberto |
author_facet |
Santiago-Moreno, Julián Pequeño, Belén Martinez-Madrid, Belen Castaño, Cristina Bóveda, Paula Velázquez, Rosario Toledano-Díaz, Adolfo Álvarez-Rodríguez, Manuel Rodríguez-Martínez, Heriberto |
author_sort |
Santiago-Moreno, Julián |
title |
Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations |
title_short |
Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations |
title_full |
Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations |
title_fullStr |
Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations |
title_full_unstemmed |
Expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations |
title_sort |
expression of aquaglyceroporins in spermatozoa from wild ruminants is influenced by photoperiod and thyroxine concentrations |
publisher |
Multidisciplinary Digital Publishing Institute |
publishDate |
2022-03-03 |
url |
http://hdl.handle.net/20.500.12792/6188 http://hdl.handle.net/10261/289650 |
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