A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa
Egg yolk-based diluents provide adequate cryoprotection for the sperm of several mammalian species. Traditionally, chicken egg yolk has been used as additive for the freeze preservation of spermatozoa because of its wide availability. Variations in the chemical composition of the egg yolk of different avian species appear to influence the protection afforded during cooling, freezing, and thawing. The aim of the present study was to assess the use of quail egg yolk as a novel additive for the epididymal spermatozoa of a threatened wild ruminant species-the Spanish ibex-and to compare its efficacy with chicken egg yolk. Epididymal spermatozoa were diluted using a Tris-citric acid-glucose medium (TCG) composed of 3.8% Tris (w v-1), 2.2% citric acid (w v-1), 0.6% glucose (w v-1), 5% glycerol (v v-1), and 6% egg yolk (v v-1). Sperm masses from the right epididymes were diluted with TCG-6% chicken egg yolk medium, while those from the left were diluted with TCG-6% quail egg yolk. The thawed spermatozoa preserved with TCG-6% quail egg yolk extender exhibited lower motility (P < 0.001), membrane integrity (P < 0.001), and viability (P < 0.01) than those diluted with the TCG-6% chicken egg yolk extender. The fertility of spermatozoa frozen in TCG-6% chicken egg yolk tended to be higher than in those frozen with TCG-6% quail egg yolk (63.3% vs 36.4%, P = 0.19). These results show that quail egg yolk offers no advantages over chicken egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa. © 2008 Elsevier Inc. All rights reserved.
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2008
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Subjects: | Cryopreservation, Diluent, Egg yolk, Epididymal spermatozoa, Ibex, |
Online Access: | http://hdl.handle.net/20.500.12792/4171 http://hdl.handle.net/10261/289572 |
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dig-inia-es-10261-2895722023-02-16T12:21:31Z A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa Santiago Moreno, Julián Coloma, M. A. Toledano Díaz, Adolfo Gómez-Brunet, A. Pulido-Pastor, A. Zamora-Soria, A. Carrizosa, J. A. Urrutia, B. López Sebastián, Antonio Cryopreservation Diluent Egg yolk Epididymal spermatozoa Ibex Egg yolk-based diluents provide adequate cryoprotection for the sperm of several mammalian species. Traditionally, chicken egg yolk has been used as additive for the freeze preservation of spermatozoa because of its wide availability. Variations in the chemical composition of the egg yolk of different avian species appear to influence the protection afforded during cooling, freezing, and thawing. The aim of the present study was to assess the use of quail egg yolk as a novel additive for the epididymal spermatozoa of a threatened wild ruminant species-the Spanish ibex-and to compare its efficacy with chicken egg yolk. Epididymal spermatozoa were diluted using a Tris-citric acid-glucose medium (TCG) composed of 3.8% Tris (w v-1), 2.2% citric acid (w v-1), 0.6% glucose (w v-1), 5% glycerol (v v-1), and 6% egg yolk (v v-1). Sperm masses from the right epididymes were diluted with TCG-6% chicken egg yolk medium, while those from the left were diluted with TCG-6% quail egg yolk. The thawed spermatozoa preserved with TCG-6% quail egg yolk extender exhibited lower motility (P < 0.001), membrane integrity (P < 0.001), and viability (P < 0.01) than those diluted with the TCG-6% chicken egg yolk extender. The fertility of spermatozoa frozen in TCG-6% chicken egg yolk tended to be higher than in those frozen with TCG-6% quail egg yolk (63.3% vs 36.4%, P = 0.19). These results show that quail egg yolk offers no advantages over chicken egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa. © 2008 Elsevier Inc. All rights reserved. 2023-02-16T12:21:31Z 2023-02-16T12:21:31Z 2008 artículo Cryobiology 57(1): 25-29 (2008) 0011-2240 http://hdl.handle.net/20.500.12792/4171 http://hdl.handle.net/10261/289572 10.1016/j.cryobiol.2008.05.001 en none Elsevier |
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Cryopreservation Diluent Egg yolk Epididymal spermatozoa Ibex Cryopreservation Diluent Egg yolk Epididymal spermatozoa Ibex |
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Cryopreservation Diluent Egg yolk Epididymal spermatozoa Ibex Cryopreservation Diluent Egg yolk Epididymal spermatozoa Ibex Santiago Moreno, Julián Coloma, M. A. Toledano Díaz, Adolfo Gómez-Brunet, A. Pulido-Pastor, A. Zamora-Soria, A. Carrizosa, J. A. Urrutia, B. López Sebastián, Antonio A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa |
description |
Egg yolk-based diluents provide adequate cryoprotection for the sperm of several mammalian species. Traditionally, chicken egg yolk has been used as additive for the freeze preservation of spermatozoa because of its wide availability. Variations in the chemical composition of the egg yolk of different avian species appear to influence the protection afforded during cooling, freezing, and thawing. The aim of the present study was to assess the use of quail egg yolk as a novel additive for the epididymal spermatozoa of a threatened wild ruminant species-the Spanish ibex-and to compare its efficacy with chicken egg yolk. Epididymal spermatozoa were diluted using a Tris-citric acid-glucose medium (TCG) composed of 3.8% Tris (w v-1), 2.2% citric acid (w v-1), 0.6% glucose (w v-1), 5% glycerol (v v-1), and 6% egg yolk (v v-1). Sperm masses from the right epididymes were diluted with TCG-6% chicken egg yolk medium, while those from the left were diluted with TCG-6% quail egg yolk. The thawed spermatozoa preserved with TCG-6% quail egg yolk extender exhibited lower motility (P < 0.001), membrane integrity (P < 0.001), and viability (P < 0.01) than those diluted with the TCG-6% chicken egg yolk extender. The fertility of spermatozoa frozen in TCG-6% chicken egg yolk tended to be higher than in those frozen with TCG-6% quail egg yolk (63.3% vs 36.4%, P = 0.19). These results show that quail egg yolk offers no advantages over chicken egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa. © 2008 Elsevier Inc. All rights reserved. |
format |
artículo |
topic_facet |
Cryopreservation Diluent Egg yolk Epididymal spermatozoa Ibex |
author |
Santiago Moreno, Julián Coloma, M. A. Toledano Díaz, Adolfo Gómez-Brunet, A. Pulido-Pastor, A. Zamora-Soria, A. Carrizosa, J. A. Urrutia, B. López Sebastián, Antonio |
author_facet |
Santiago Moreno, Julián Coloma, M. A. Toledano Díaz, Adolfo Gómez-Brunet, A. Pulido-Pastor, A. Zamora-Soria, A. Carrizosa, J. A. Urrutia, B. López Sebastián, Antonio |
author_sort |
Santiago Moreno, Julián |
title |
A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa |
title_short |
A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa |
title_full |
A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa |
title_fullStr |
A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa |
title_full_unstemmed |
A comparison of the protective action of chicken and quail egg yolk in the cryopreservation of Spanish ibex epididymal spermatozoa |
title_sort |
comparison of the protective action of chicken and quail egg yolk in the cryopreservation of spanish ibex epididymal spermatozoa |
publisher |
Elsevier |
publishDate |
2008 |
url |
http://hdl.handle.net/20.500.12792/4171 http://hdl.handle.net/10261/289572 |
work_keys_str_mv |
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