High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection
12 Pág.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
John Wiley & Sons
2021-04-26
|
| Subjects: | Arabidopsis thaliana, Nicotiana benthamiana, Turnip mosaic virus, CERES, Defence, Leucine-rich repeat protein, Eukaryotic translation initiation factor 4E, Translation regulation, |
| Online Access: | http://hdl.handle.net/10261/288475 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 https://api.elsevier.com/content/abstract/scopus_id/85105913702 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-inia-es-10261-288475 |
|---|---|
| record_format |
koha |
| spelling |
dig-inia-es-10261-2884752024-05-17T20:36:44Z High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection Toribio, René Muñoz, Alfonso Sánchez, Flora Ponz Ascaso, Fernando Castellano Moreno, María Mar European Commission Comunidad de Madrid Agencia Estatal de Investigación (España) Toribio, René [0000-0002-4847-629X] Muñoz, Alfonso [0000-0001-6399-3853] Ponz Ascaso, Fernando [0000-0003-0344-4363] Castellano Moreno, María Mar [0000-0003-0339-4247] Arabidopsis thaliana Nicotiana benthamiana Turnip mosaic virus CERES Defence Leucine-rich repeat protein Eukaryotic translation initiation factor 4E Translation regulation 12 Pág. Mutations in the eukaryotic translation initiation factors eIF4E and eIF(iso)4E confer potyvirus resistance in a range of plant hosts. This supports the notion that, in addition to their role in translation of cellular mRNAs, eIF4E isoforms are also essential for the potyvirus cycle. CERES is a plant eIF4E- and eIF(iso)4E-binding protein that, through its binding to the eIF4Es, modulates translation initiation; however, its possible role in potyvirus resistance is unknown. In this article, we analyse if the ectopic expression of AtCERES is able to interfere with turnip mosaic virus replication in plants. Our results demonstrate that, during infection, the ectopic expression of CERES in Nicotiana benthamiana promotes the development of a mosaic phenotype when it is accumulated to moderate levels, but induces veinal necrosis when it is accumulated to higher levels. This necrotic process resembles a hypersensitive response (HR)-like response that occurs with different HR hallmarks. Remarkably, Arabidopsis plants inoculated with a virus clone that promotes high expression of CERES do not show signs of infection. These final phenotypical outcomes are independent of the capacity of CERES to bind to eIF4E. All these data suggest that CERES, most likely due to its leucine-rich repeat nature, could act as a resistance protein, able to promote a range of different defence responses when it is highly overexpressed from viral constructs. This research has received funding from the European Research Council under the European Union’s Seventh Framework Programme (FP/2007-2013)/ERC Grant Agreement n. 260468 to MMC and from the grant S2013-ABI2734 from CAM. In addition, this work has been financially supported by RTI2018-095946-B100 from MICIU and by the ‘Severo Ochoa Programme for Centres of Excellence in R&D’ from the Agencia Estatal de Investigación of Spain (grant SEV-2016-0672 [2017–2021]) to the CBGP. In the frame of this latter program, RT was supported with a postdoctoral contract. Peer reviewed 2023-02-07T11:28:20Z 2023-02-07T11:28:20Z 2021-04-26 artículo http://purl.org/coar/resource_type/c_6501 Plant Journal 107(1): 256-267 (2021) 0960-7412 http://hdl.handle.net/10261/288475 10.1111/tpj.15290 1365-313X http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 33899980 2-s2.0-85105913702 https://api.elsevier.com/content/abstract/scopus_id/85105913702 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP7/260468 S2013/ABI2734 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-095946-B-I00/ES/ESTUDIO DE LA RESPUESTA DE LAS PLANTAS A AUMENTOS DE TEMPERATURA MODERADOS Y SU APLICACION BIOTECNOLOGICA/ Centro de Biotecnología y Genómica de Plantas Publisher's version https://doi.org/10.1111/tpj.15290 Sí open John Wiley & Sons |
| institution |
INIA ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-inia-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del INIA España |
| language |
English |
| topic |
Arabidopsis thaliana Nicotiana benthamiana Turnip mosaic virus CERES Defence Leucine-rich repeat protein Eukaryotic translation initiation factor 4E Translation regulation Arabidopsis thaliana Nicotiana benthamiana Turnip mosaic virus CERES Defence Leucine-rich repeat protein Eukaryotic translation initiation factor 4E Translation regulation |
| spellingShingle |
Arabidopsis thaliana Nicotiana benthamiana Turnip mosaic virus CERES Defence Leucine-rich repeat protein Eukaryotic translation initiation factor 4E Translation regulation Arabidopsis thaliana Nicotiana benthamiana Turnip mosaic virus CERES Defence Leucine-rich repeat protein Eukaryotic translation initiation factor 4E Translation regulation Toribio, René Muñoz, Alfonso Sánchez, Flora Ponz Ascaso, Fernando Castellano Moreno, María Mar High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection |
| description |
12 Pág. |
| author2 |
European Commission |
| author_facet |
European Commission Toribio, René Muñoz, Alfonso Sánchez, Flora Ponz Ascaso, Fernando Castellano Moreno, María Mar |
| format |
artículo |
| topic_facet |
Arabidopsis thaliana Nicotiana benthamiana Turnip mosaic virus CERES Defence Leucine-rich repeat protein Eukaryotic translation initiation factor 4E Translation regulation |
| author |
Toribio, René Muñoz, Alfonso Sánchez, Flora Ponz Ascaso, Fernando Castellano Moreno, María Mar |
| author_sort |
Toribio, René |
| title |
High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection |
| title_short |
High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection |
| title_full |
High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection |
| title_fullStr |
High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection |
| title_full_unstemmed |
High overexpression of CERES, a plant regulator of translation, induces different phenotypical defence responses during TuMV infection |
| title_sort |
high overexpression of ceres, a plant regulator of translation, induces different phenotypical defence responses during tumv infection |
| publisher |
John Wiley & Sons |
| publishDate |
2021-04-26 |
| url |
http://hdl.handle.net/10261/288475 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100012818 https://api.elsevier.com/content/abstract/scopus_id/85105913702 |
| work_keys_str_mv |
AT toribiorene highoverexpressionofceresaplantregulatoroftranslationinducesdifferentphenotypicaldefenceresponsesduringtumvinfection AT munozalfonso highoverexpressionofceresaplantregulatoroftranslationinducesdifferentphenotypicaldefenceresponsesduringtumvinfection AT sanchezflora highoverexpressionofceresaplantregulatoroftranslationinducesdifferentphenotypicaldefenceresponsesduringtumvinfection AT ponzascasofernando highoverexpressionofceresaplantregulatoroftranslationinducesdifferentphenotypicaldefenceresponsesduringtumvinfection AT castellanomorenomariamar highoverexpressionofceresaplantregulatoroftranslationinducesdifferentphenotypicaldefenceresponsesduringtumvinfection |
| _version_ |
1802819698868355072 |