Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions
13 Pág. Instituto de Ciencias Forestales (ICIFOR)
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| Format: | artículo biblioteca |
| Language: | English |
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Multidisciplinary Digital Publishing Institute
2021-09-22
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| Subjects: | Monterey pine, P. parvispora, PAL, PR3, PR5, Phytophthora xcambivora, Gene expression, Housekeeping genes, Pitch canker disease, Plant- oomycetes- fungal interactions, Plant-defense molecular mechanisms, |
| Online Access: | http://hdl.handle.net/10261/283861 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85115366367 |
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dig-inia-es-10261-2838612024-05-16T20:41:31Z Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions Aloi, Francesco Zamora-Ballesteros, Cristina Martín-García, Jorge Díez, Julio Javier Cacciola, Santa Olga European Commission Università degli Studi di Catania Aloi, Francesco [0000-0002-0876-0763] Zamora-Ballesteros, Cristina [0000-0002-9728-5553] Martín-García, Jorge [0000-0002-3057-3710] Díez, Julio Javier [0000-0003-0558-8141] Cacciola, Santa Olga [0000-0001-7926-3601] Monterey pine P. parvispora PAL PR3 PR5 Phytophthora xcambivora Gene expression Housekeeping genes Pitch canker disease Plant- oomycetes- fungal interactions Plant-defense molecular mechanisms 13 Pág. Instituto de Ciencias Forestales (ICIFOR) This study investigated the complex phenotypic and genetic response of Monterey pine (Pinus radiata) seedlings to co-infections by F. circinatum, the causal agent of pine pitch canker disease, and the oomycetes Phytophthora xcambivora and P. parvispora. Monterey pine seedlings were wound-inoculated with each single pathogen and with the combinations F. circinatum/P. xcambivora and F. circinatum/P. parvispora. Initially, seedlings inoculated only with F. circinatum showed less severe symptoms than seedlings co-inoculated or inoculated only with P. xcambivora or P. parvispora. However, 30 days post-inoculation (dpi), all inoculated seedlings, including those inoculated only with F. circinatum, showed severe symptoms with no significant differences among treatments. The transcriptomic profiles of three genes encoding pathogenesis-related proteins, i.e., chitinase (PR3), thaumatin-like protein (PR5), phenylalanine ammonia-lyase (PAL), and the pyruvate decarboxylase (PDC)-encoding gene were analyzed at various time intervals after inoculation. In seedlings inoculated with single pathogens, F. circinatum stimulated the up-regulation of all genes, while between the two oomycetes, only P. xcambivora induced significant up-regulations. In seedlings co-inoculated with F. circinatum and P.xcambivora or P. parvispora none of the genes showed a significant over-expression 4 dpi. In contrast, at 11 dpi, significant up-regulation was observed for PR5 in the combination F. circinatum/P.xcambivora and PDC in the combination F. circinatum/P. parvispora, thus suggesting a possible synergism of multiple infections in triggering this plant defense mechanism. This article is based upon the activity of COST Action FP1406 PINESTRENGTH (Pine Pitch Canker strategies for management of Gibberella circinata in greenhouses and forests) supported by COST (European Cooperation in Science and Technology); this research was funded by the University of Catania, Italy “Investigation of phytopathological problems of the main Sicilian productive contexts and eco-sustainable defense strategies (MEDIT-ECO)” “PiaCeRi-PIAno di inCEntivi per la Ricerca di Ateneo 2020-22 linea 2” “5A722192155”; by the project “Smart and innovative packaging, postharvest rot management and shipping of organic citrus fruit (BiOrangePack)” Partnership for Research and Innovation in the Mediterranea Area (PRIMA)-H2020 (E69C20000130001). F.A. has been granted a Ph.D. fellowship “Scienze Agrarie, Alimentari, Forestali e Ambientali—XXXIII cycle”, University of Palermo. Peer reviewed 2022-11-25T12:40:51Z 2022-11-25T12:40:51Z 2021-09-22 artículo http://purl.org/coar/resource_type/c_6501 Plants 10(10): 1976 (2021) 2223-7747 http://hdl.handle.net/10261/283861 10.3390/plants10101976 http://dx.doi.org/10.13039/501100000780 34685785 2-s2.0-85115366367 https://api.elsevier.com/content/abstract/scopus_id/85115366367 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/FP1406/ PINESTRENGTH Plants (Basel, Switzerland) Publisher's version https://doi.org/10.3390/plants10101976 Sí open Multidisciplinary Digital Publishing Institute |
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Monterey pine P. parvispora PAL PR3 PR5 Phytophthora xcambivora Gene expression Housekeeping genes Pitch canker disease Plant- oomycetes- fungal interactions Plant-defense molecular mechanisms Monterey pine P. parvispora PAL PR3 PR5 Phytophthora xcambivora Gene expression Housekeeping genes Pitch canker disease Plant- oomycetes- fungal interactions Plant-defense molecular mechanisms |
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Monterey pine P. parvispora PAL PR3 PR5 Phytophthora xcambivora Gene expression Housekeeping genes Pitch canker disease Plant- oomycetes- fungal interactions Plant-defense molecular mechanisms Monterey pine P. parvispora PAL PR3 PR5 Phytophthora xcambivora Gene expression Housekeeping genes Pitch canker disease Plant- oomycetes- fungal interactions Plant-defense molecular mechanisms Aloi, Francesco Zamora-Ballesteros, Cristina Martín-García, Jorge Díez, Julio Javier Cacciola, Santa Olga Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions |
| description |
13 Pág.
Instituto de Ciencias Forestales (ICIFOR) |
| author2 |
European Commission |
| author_facet |
European Commission Aloi, Francesco Zamora-Ballesteros, Cristina Martín-García, Jorge Díez, Julio Javier Cacciola, Santa Olga |
| format |
artículo |
| topic_facet |
Monterey pine P. parvispora PAL PR3 PR5 Phytophthora xcambivora Gene expression Housekeeping genes Pitch canker disease Plant- oomycetes- fungal interactions Plant-defense molecular mechanisms |
| author |
Aloi, Francesco Zamora-Ballesteros, Cristina Martín-García, Jorge Díez, Julio Javier Cacciola, Santa Olga |
| author_sort |
Aloi, Francesco |
| title |
Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions |
| title_short |
Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions |
| title_full |
Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions |
| title_fullStr |
Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions |
| title_full_unstemmed |
Co-Infections by Fusarium circinatum and Phytophthora spp. on Pinus radiata: Complex Phenotypic and Molecular Interactions |
| title_sort |
co-infections by fusarium circinatum and phytophthora spp. on pinus radiata: complex phenotypic and molecular interactions |
| publisher |
Multidisciplinary Digital Publishing Institute |
| publishDate |
2021-09-22 |
| url |
http://hdl.handle.net/10261/283861 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85115366367 |
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