Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs

Rangel, Giselle; Bárcena, Juan; Moreno, Noelia; Mata, Carlos P.; Castón, José R.; Alejo, Alí; Blanco, Esther; Ministerio de Ciencia e Innovación (España)

Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs

22 Pág. Centro de Investigación en Sanidad Animal (CISA)

Saved in:

Bibliographic Details
Main Authors:	Rangel, Giselle, Bárcena, Juan, Moreno, Noelia, Mata, Carlos P., Castón, José R., Alejo, Alí, Blanco, Esther
Other Authors:	Ministerio de Ciencia e Innovación (España)
Format:	artículo biblioteca
Language:	English
Published:	Multidisciplinary Digital Publishing Institute 2021-05-07
Subjects:	B-cell epitope, FMDV, RHDV, T-cell epitope, Chimeric VLPs, Immune response, Nananoparticles, Pig, Vaccine platform, Virus-like particles (VLPs),
Online Access:	http://hdl.handle.net/10261/283778 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85105966307
Tags:	Add Tag No Tags, Be the first to tag this record!

id	dig-inia-es-10261-283778
record_format	koha
spelling	dig-inia-es-10261-2837782024-05-18T20:40:47Z Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs Rangel, Giselle Bárcena, Juan Moreno, Noelia Mata, Carlos P. Castón, José R. Alejo, Alí Blanco, Esther Ministerio de Ciencia e Innovación (España) Comunidad de Madrid European Commission Fundación la Caixa Rangel, Giselle [0000-0001-6825-3803] Bárcena, Juan [0000-0001-6050-0654] Mata, Carlos P [0000-0003-3381-7431] Castón, José R. [0000-0003-2350-9048] Alejo, Alí [0000-0002-1613-6063] Blanco, Esther [0000-0001-8413-4222] B-cell epitope FMDV RHDV T-cell epitope Chimeric VLPs Immune response Nananoparticles Pig Vaccine platform Virus-like particles (VLPs) 22 Pág. Centro de Investigación en Sanidad Animal (CISA) Currently there is a clear trend towards the establishment of virus-like particles (VLPs) as a powerful tool for vaccine development. VLPs are tunable nanoparticles that can be engineered to be used as platforms for multimeric display of foreign antigens. We have previously reported that VLPs derived from rabbit hemorrhagic disease virus (RHDV) constitute an excellent vaccine vector, capable of inducing specific protective immune responses against inserted heterologous T-cytotoxic and B-cell epitopes. Here, we evaluate the ability of chimeric RHDV VLPs to elicit immune response and protection against Foot-and-Mouth disease virus (FMDV), one of the most devastating livestock diseases. For this purpose, we generated a set of chimeric VLPs containing two FMDV-derived epitopes: a neutralizing B-cell epitope (VP1 (140-158)) and a T-cell epitope [3A (21-35)]. The epitopes were inserted joined or individually at two different locations within the RHDV capsid protein. The immunogenicity and protection potential of the chimeric VLPs were analyzed in the mouse and pig models. Herein we show that the RHDV engineered VLPs displaying FMDV-derived epitopes elicit a robust neutralizing immune response in mice and pigs, affording partial clinical protection against an FMDV challenge in pigs. This work was supported by Spanish Ministry of Science and Innovation (grants AGL2016-76445-R to EB and AA; PID2019-107145RB-I00 to EB and BFU2017-88736- to JRC) and Comunidad de Madrid co-financed with ECFEDER funds (P2018/BAA-4370 PLATESA 2 to EB and P2018/NMT-4389 to JRC). GR and NM were holders of a PhD fellowship from the Spanish Ministry of Science and Innovation (FPI programme) and CPM was a PhD fellow of the La Caixa Foundation International Fellowship Program (La Caixa/CNB). Peer reviewed 2022-11-25T07:55:53Z 2022-11-25T07:55:53Z 2021-05-07 artículo http://purl.org/coar/resource_type/c_6501 Vaccines 9(5): 370 (2021) 2076-393X http://hdl.handle.net/10261/283778 10.3390/vaccines9050470 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 34066934 2-s2.0-85105966307 https://api.elsevier.com/content/abstract/scopus_id/85105966307 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//AGL2016-76445-R info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-107145RB-I00/ES/PREVENCION Y CONTROL DE ENFERMEDADES VIRICAS GANADERAS MEDIANTE BIONANOPARTICULAS: PSEUDOPARTICULAS VIRALES MODIFICADAS Y VESICULAS EXTRACELULARES/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2017-88736-R/ES/VIROTECNOLOGIA ESTRUCTURAL: DESARROLLO DE ENSAMBLADOS VIRALES HIBRIDOS CON NUEVAS PROPIEDADES/ S2018/BAA-4370/PLATESA S2018/NMT-4389 Vaccines Publisher's version https://doi.org/10.3390/vaccines9050470 Sí open Multidisciplinary Digital Publishing Institute
institution	INIA ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-inia-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del INIA España
language	English
topic	B-cell epitope FMDV RHDV T-cell epitope Chimeric VLPs Immune response Nananoparticles Pig Vaccine platform Virus-like particles (VLPs) B-cell epitope FMDV RHDV T-cell epitope Chimeric VLPs Immune response Nananoparticles Pig Vaccine platform Virus-like particles (VLPs)
spellingShingle	B-cell epitope FMDV RHDV T-cell epitope Chimeric VLPs Immune response Nananoparticles Pig Vaccine platform Virus-like particles (VLPs) B-cell epitope FMDV RHDV T-cell epitope Chimeric VLPs Immune response Nananoparticles Pig Vaccine platform Virus-like particles (VLPs) Rangel, Giselle Bárcena, Juan Moreno, Noelia Mata, Carlos P. Castón, José R. Alejo, Alí Blanco, Esther Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs
description	22 Pág. Centro de Investigación en Sanidad Animal (CISA)
author2	Ministerio de Ciencia e Innovación (España)
author_facet	Ministerio de Ciencia e Innovación (España) Rangel, Giselle Bárcena, Juan Moreno, Noelia Mata, Carlos P. Castón, José R. Alejo, Alí Blanco, Esther
format	artículo
topic_facet	B-cell epitope FMDV RHDV T-cell epitope Chimeric VLPs Immune response Nananoparticles Pig Vaccine platform Virus-like particles (VLPs)
author	Rangel, Giselle Bárcena, Juan Moreno, Noelia Mata, Carlos P. Castón, José R. Alejo, Alí Blanco, Esther
author_sort	Rangel, Giselle
title	Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs
title_short	Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs
title_full	Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs
title_fullStr	Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs
title_full_unstemmed	Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs
title_sort	chimeric rhdv virus-like particles displaying foot-and-mouth disease virus epitopes elicit neutralizing antibodies and confer partial protection in pigs
publisher	Multidisciplinary Digital Publishing Institute
publishDate	2021-05-07
url	http://hdl.handle.net/10261/283778 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 https://api.elsevier.com/content/abstract/scopus_id/85105966307
work_keys_str_mv	AT rangelgiselle chimericrhdvviruslikeparticlesdisplayingfootandmouthdiseasevirusepitopeselicitneutralizingantibodiesandconferpartialprotectioninpigs AT barcenajuan chimericrhdvviruslikeparticlesdisplayingfootandmouthdiseasevirusepitopeselicitneutralizingantibodiesandconferpartialprotectioninpigs AT morenonoelia chimericrhdvviruslikeparticlesdisplayingfootandmouthdiseasevirusepitopeselicitneutralizingantibodiesandconferpartialprotectioninpigs AT matacarlosp chimericrhdvviruslikeparticlesdisplayingfootandmouthdiseasevirusepitopeselicitneutralizingantibodiesandconferpartialprotectioninpigs AT castonjoser chimericrhdvviruslikeparticlesdisplayingfootandmouthdiseasevirusepitopeselicitneutralizingantibodiesandconferpartialprotectioninpigs AT alejoali chimericrhdvviruslikeparticlesdisplayingfootandmouthdiseasevirusepitopeselicitneutralizingantibodiesandconferpartialprotectioninpigs AT blancoesther chimericrhdvviruslikeparticlesdisplayingfootandmouthdiseasevirusepitopeselicitneutralizingantibodiesandconferpartialprotectioninpigs
_version_	1802819672960139264

Chimeric RHDV Virus-Like Particles Displaying Foot-and-Mouth Disease Virus Epitopes Elicit Neutralizing Antibodies and Confer Partial Protection in Pigs

Similar Items

Resource Map