Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation
9 Pág. Departamento de Reproducción animal. (INIA)
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| Language: | English |
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Elsevier
2021-11-14
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| Subjects: | Cryopreservation, Sperm, Vitrification, Wolf, |
| Online Access: | http://hdl.handle.net/10261/281011 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85119258041 |
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dig-inia-es-10261-2810112024-10-29T21:37:15Z Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation Cerdeira, J. Castaño, Cristina Pérez, J.F. Marcos-Beltrán, J. L. Guerra, Rafael López-Fernández, Matilde Torija, E Rodríguez, Ana Martínez-Nevado, Eva Toledano-Díaz, A. Sánchez-Calabuig, M. J. Santiago Moreno, Julián Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) European Commission Castaño, C [0000-0003-1134-1436] Torija, E [0000-0003-3859-9699] Martínez-Nevado, E [0000-0002-8994-4205] Toledano Díaz, Adolfo [0000-0001-6679-485X] Santiago Moreno, Julián [0000-0001-5551-8120] López-Fernández, Matilde [0000-0002-6265-9834] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Cryopreservation Sperm Vitrification Wolf 9 Pág. Departamento de Reproducción animal. (INIA) Sperm vitrification is a simple, inexpensive method that allows the cryopreservation of sperm in the field and for endangered species is a useful alternative to conventional freezing. The study, therefore, is focused on the suitability of vitrification for cryopreserving Iberian wolf sperm and utilizing plasma testosterone concentration as a marker for procedure efficacy. Sperm and blood samples were collected from 17 wolves. There were 14 samples suitable for cryopreservation (12 ejaculated and two epididymal). Immediately after collection, these samples were proportioned into two aliquots for conventional freezing using a Tris-citric acid-glucose based extender (TCG) or vitrification utilizing an animal protein free extender (HTF®). Vitrification occurred by directly plunging a sperm suspension into liquid nitrogen. Sperm were assessed for motility, membrane integrity, acrosomal status and DNA integrity before and after cryopreservation. With both techniques, there were similar post-thaw/warming results (P > 0.05) with respect to progressive motility, kinetic variables VCL, VSL, VAP and BCF, DNA fragmentation, sperm membrane functionality and morphological abnormalities. Total motile sperm, progression ratios LIN, STR, and WOB, the ALH, sperm viability and sperm with intact membrane and acrosome were greater (P < 0.05) in the conventional frozen-thawed sperm than vitrified-warmed sperm. Plasma testosterone concentrations varied from 0.0 ng/mL to 7.7 ng/mL. For epididymal sperm, sperm motility and viability following thawing were greater in vitrified-warmed samples than conventionally-frozen samples; however, small sample numbers precluded statistical analysis. When considered together, these results indicate vitrification may be a possible alternative for wolf sperm cryopreservation. This research was funded by MCINN/AEI/FEDER grant AGL2017-85753-R and by the Fundación Parques Reunidos INIA agreement CC19-096. Peer reviewed 2022-10-14T07:41:30Z 2022-10-14T07:41:30Z 2021-11-14 artículo http://purl.org/coar/resource_type/c_6501 Animal Reproduction Science 235: 106887 (2021) 0378-4320 http://hdl.handle.net/10261/281011 10.1016/j.anireprosci.2021.106887 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 34798241 2-s2.0-85119258041 https://api.elsevier.com/content/abstract/scopus_id/85119258041 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-85753-R/ES/VARIACIONES EN LA EFICACIA DE LA CRIOPRESERVACION DE ESPERMATOZOIDES DE PEQUEÑOS RUMIANTES COMO RESPUESTA A LOS CAMBIOS DE HORMONAS TIROIDEAS Y LA EXPRESION DE ACUAPORINAS/ Animal reproduction science Publisher's version https://doi.org/10.1016/j.anireprosci.2021.106887 Sí open Elsevier |
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Cryopreservation Sperm Vitrification Wolf Cryopreservation Sperm Vitrification Wolf |
| spellingShingle |
Cryopreservation Sperm Vitrification Wolf Cryopreservation Sperm Vitrification Wolf Cerdeira, J. Castaño, Cristina Pérez, J.F. Marcos-Beltrán, J. L. Guerra, Rafael López-Fernández, Matilde Torija, E Rodríguez, Ana Martínez-Nevado, Eva Toledano-Díaz, A. Sánchez-Calabuig, M. J. Santiago Moreno, Julián Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation |
| description |
9 Pág.
Departamento de Reproducción animal. (INIA) |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Cerdeira, J. Castaño, Cristina Pérez, J.F. Marcos-Beltrán, J. L. Guerra, Rafael López-Fernández, Matilde Torija, E Rodríguez, Ana Martínez-Nevado, Eva Toledano-Díaz, A. Sánchez-Calabuig, M. J. Santiago Moreno, Julián |
| format |
artículo |
| topic_facet |
Cryopreservation Sperm Vitrification Wolf |
| author |
Cerdeira, J. Castaño, Cristina Pérez, J.F. Marcos-Beltrán, J. L. Guerra, Rafael López-Fernández, Matilde Torija, E Rodríguez, Ana Martínez-Nevado, Eva Toledano-Díaz, A. Sánchez-Calabuig, M. J. Santiago Moreno, Julián |
| author_sort |
Cerdeira, J. |
| title |
Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation |
| title_short |
Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation |
| title_full |
Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation |
| title_fullStr |
Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation |
| title_full_unstemmed |
Vitrification of Iberian wolf (Canis lupus signatus) sperm: A possible alternative to conventional cryopreservation |
| title_sort |
vitrification of iberian wolf (canis lupus signatus) sperm: a possible alternative to conventional cryopreservation |
| publisher |
Elsevier |
| publishDate |
2021-11-14 |
| url |
http://hdl.handle.net/10261/281011 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85119258041 |
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