Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle
15 Pág. Departamento de Reproducción Animal (INIA)
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| Format: | artículo biblioteca |
| Language: | English |
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John Wiley & Sons
2021-12
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| Subjects: | Estrous cycle, Extracellular vesicles, MicroRNA, Oviduct, Uterus, |
| Online Access: | http://hdl.handle.net/10261/280999 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85120716510 |
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dig-inia-es-10261-2809992024-05-18T20:40:58Z Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle Hamdi, M. Cañón-Beltrán, Karina Mazzarella, Rosane Cajas, Yulia N. Leal, Cláudia Lima Verde Gutiérrez-Adán, Alfonso González, Encina M. da Silveira, Juliano C. Rizos, Dimitrios Ministerio de Ciencia e Innovación (España) Secretaría de Educación Superior, Ciencia y Tecnología (Ecuador) Sao Paulo Research Foundation Ministerio de Ciencia, Tecnología e Innovación (Colombia) Hamdi, M. [0000-0002-2792-8293] Cañón-Beltrán, Karina [0000-0002-0279-0857] Mazzarella, Rosane [0000-0001-6860-8897] Cajas, Yulia N. [0000-0001-9791-6733] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] González, Encina M. [0000-0002-6217-866X] da Silveira, Juliano C. [0000-0002-4796-6393] Rizos, Dimitrios [0000-0001-6813-3940] Estrous cycle Extracellular vesicles MicroRNA Oviduct Uterus 15 Pág. Departamento de Reproducción Animal (INIA) Extracellular vesicles (EVs) found in various biological fluids and particularly in reproductive fluids, have gained considerable attention for their possible role in cell- to- cell communication. Among, the different bioactive molecules cargos of EVs, MicroRNAs (miRNAs) are emerging as promising diagnostic biomarkers with high clinical potential. Aiming to understand the roles of EVs in bovine reproductive tract, we intended to characterize and profile the EVs of oviduct and uterine fluids (OF-EVs, UF-EVs) and their miRNA across the estrous cycle. Nanoparticle tracking analysis and transmission electron microscopy confirmed the existence of small EV population in OF and UF at all stages, (size between 30 and 200 nm; concentration: 3.4 × 1010 EVs/ml and 6.0 × 1010 EVs/ml for OF and UF, respectively, regardless of stage). The identification of EV markers (CD9, HSP70, and ALIX proteins) was confirmed by western blot. The miRNA analysis revealed the abundance of 310 and 351 miRNAs in OF-EVs and UF-EVs, respectively. Nine miRNAs were differentially abundant in OF-EVs between stages of the cycle, eight of them displayed a progressive increase from S1 to S4 (p < .05). In UF-EVs, a total of 14 miRNAs were differentially abundant between stages. Greater differences were observed between stage 1 (S1) and stage 3 (S3), with 11 miRNAs enriched in S3 compared to S1. Functional enrichment analysis revealed the involvement of these miRNAs in relevant pathways such as cell signaling, intercellular junctions, and reproductive functions that may be implicated in oviduct and uterus modulation across the cycle, but also in their preparation for embryo/conceptus presence and development. This work was supported by the Spanish Ministry of Science and Innovation (PID2019‐111641RB‐I00 to DR, and RTI2018‐093548‐B‐I00 to AGA); Minciencias‐Colombia (848‐2019) Postdoctoral Fellowship contract to KC‐B; Secretaría de Educación Superior, Ciencia y Tecnología e Innovación (SENESCYT‐Ecuador) to YNC; Sao Paulo Research Foundation, Brazil (FAPESP; #2017/20339‐3 and CNPq‐Brazil 304276/2018‐9 to CLVL; #2019/04981‐2 to RM; 2014/22887‐0 and 2015/21829‐9 to JCDS). Peer reviewed 2022-10-13T13:28:35Z 2022-10-13T13:28:35Z 2021-12 artículo http://purl.org/coar/resource_type/c_6501 FASEB Journal - Federation of American Societies for Experimental Biology 35(12): e22000(2021) 0892-6638 http://hdl.handle.net/10261/280999 10.1096/fj.202101023R http://dx.doi.org/10.13039/501100004837 34731497 2-s2.0-85120716510 https://api.elsevier.com/content/abstract/scopus_id/85120716510 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MICINN//PID2019‐111641RB‐I00 info:eu-repo/grantAgreement/MICINN//RTI2018‐093548‐B‐I00 FASEB journal : official publication of the Federation of American Societies for Experimental Biology Publisher's version https://doi.org/10.1096/fj.202101023R Sí open John Wiley & Sons |
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ES |
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English |
| topic |
Estrous cycle Extracellular vesicles MicroRNA Oviduct Uterus Estrous cycle Extracellular vesicles MicroRNA Oviduct Uterus |
| spellingShingle |
Estrous cycle Extracellular vesicles MicroRNA Oviduct Uterus Estrous cycle Extracellular vesicles MicroRNA Oviduct Uterus Hamdi, M. Cañón-Beltrán, Karina Mazzarella, Rosane Cajas, Yulia N. Leal, Cláudia Lima Verde Gutiérrez-Adán, Alfonso González, Encina M. da Silveira, Juliano C. Rizos, Dimitrios Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle |
| description |
15 Pág.
Departamento de Reproducción Animal (INIA) |
| author2 |
Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Hamdi, M. Cañón-Beltrán, Karina Mazzarella, Rosane Cajas, Yulia N. Leal, Cláudia Lima Verde Gutiérrez-Adán, Alfonso González, Encina M. da Silveira, Juliano C. Rizos, Dimitrios |
| format |
artículo |
| topic_facet |
Estrous cycle Extracellular vesicles MicroRNA Oviduct Uterus |
| author |
Hamdi, M. Cañón-Beltrán, Karina Mazzarella, Rosane Cajas, Yulia N. Leal, Cláudia Lima Verde Gutiérrez-Adán, Alfonso González, Encina M. da Silveira, Juliano C. Rizos, Dimitrios |
| author_sort |
Hamdi, M. |
| title |
Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle |
| title_short |
Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle |
| title_full |
Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle |
| title_fullStr |
Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle |
| title_full_unstemmed |
Characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their miRNA cargo through the estrous cycle |
| title_sort |
characterization and profiling analysis of bovine oviduct and uterine extracellular vesicles and their mirna cargo through the estrous cycle |
| publisher |
John Wiley & Sons |
| publishDate |
2021-12 |
| url |
http://hdl.handle.net/10261/280999 http://dx.doi.org/10.13039/501100004837 https://api.elsevier.com/content/abstract/scopus_id/85120716510 |
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