Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications

Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications

12 Pàg. Departamento de Medio Ambiente y Agronomía​ (INIA)

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Main Authors: Martínez-Guitarte, José Luis, Beltrán Rodríguez, Eulalia María, González Doncel, Miguel, García-Hortigüela, Pilar, Fernández, Amanda, Pablos, María Victoria
Other Authors: Agencia Estatal de Investigación (España)
Format: artículo biblioteca
Language:English
Published: Elsevier 2021-09-22
Subjects:Carbamazepine, Reclaimed water, Thyroid axis, X.laevis,
Online Access:http://hdl.handle.net/10261/280460
http://dx.doi.org/10.13039/501100011033
https://api.elsevier.com/content/abstract/scopus_id/85115797057
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spelling dig-inia-es-10261-2804602024-10-26T20:46:27Z Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications Martínez-Guitarte, José Luis Beltrán Rodríguez, Eulalia María González Doncel, Miguel García-Hortigüela, Pilar Fernández, Amanda Pablos, María Victoria Agencia Estatal de Investigación (España) Martínez-Guitarte, José Luis [0000-0002-7722-864X] Beltrán, Eulalia María [0000-0001-6385-9582] González Doncel, Miguel [0000-0002-1590-0090] Pablos, María Victoria [0000-0003-2955-5846] Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72] Carbamazepine Reclaimed water Thyroid axis X.laevis 12 Pàg. Departamento de Medio Ambiente y Agronomía​ (INIA) Reclaimed water (RW) obtained from wastewater treatment plants (WWTP) is used for irrigation, groundwater recharge, among other potential uses. Although most pollutants are removed, traces of them are frequently found, which can affect organisms and alter the environment. The presence of a myriad of contaminants in RW makes it a complex mixture with very diverse effects and interactions. A previous study, in which tadpoles were exposed to RW and RW spiked with Carbamazepine (CBZ), presented slight thyroid gland stimulation, as suggested by the development acceleration of tadpoles and histological findings in the gland provoked by RW, regardless of the CBZ concentration. To complement this study, the present work analysed the putative molecular working mechanism by selecting six genes coding for the thyroid-stimulating hormone (TSHβ), thyroid hormone metabolising enzymes (DIO2, DIO3), thyroid receptors (THRA, THRB), and a thyroid hormone-induced DNA binding protein (Kfl9). Transcriptional activity was studied by Real-Time PCR (RT-PCR) in brains, hind limbs, and tails on exposure days 1, 7, and 21. No significant differences were observed between treatments for each time point, but slight alterations were noted when the time response was analysed. The obtained results indicate that the effects of RW or RW spiked with CBZ are negligible for the genes analysed during the selected exposure periods. This work was supported by Spanish Government Grants RTI2018-094598-B-I00 and RTI2018-096046-B-C21. The authors wish to thank the WWTP managers for supplying the reclaimed water samples. Peer reviewed 2022-10-05T07:59:22Z 2022-10-05T07:59:22Z 2021-09-22 artículo http://purl.org/coar/resource_type/c_6501 Environmental Pollution 291: 118226 (2021) 0269-7491 http://hdl.handle.net/10261/280460 10.1016/j.envpol.2021.118226 http://dx.doi.org/10.13039/501100011033 34563849 2-s2.0-85115797057 https://api.elsevier.com/content/abstract/scopus_id/85115797057 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094598-B-I00/ES/CAMBIO GLOBAL Y CONTAMINACION: ESTANDARIZACION DEL ESTUDIO DE LA RESPUESTA CELULAR Y MOLECULAR ADAPTATIVA AL MULTIESTRES EN INVERTEBRADOS ACUATICOS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-094598-B-I00/ES/CAMBIO GLOBAL Y CONTAMINACION: ESTANDARIZACION DEL ESTUDIO DE LA RESPUESTA CELULAR Y MOLECULAR ADAPTATIVA AL MULTIESTRES EN INVERTEBRADOS ACUATICOS/ Environmental pollution (Barking, Essex : 1987) Publisher's version https://doi.org/10.1016/j.envpol.2021.118226 Sí open Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Carbamazepine
Reclaimed water
Thyroid axis
X.laevis
Carbamazepine
Reclaimed water
Thyroid axis
X.laevis
spellingShingle Carbamazepine
Reclaimed water
Thyroid axis
X.laevis
Carbamazepine
Reclaimed water
Thyroid axis
X.laevis
Martínez-Guitarte, José Luis
Beltrán Rodríguez, Eulalia María
González Doncel, Miguel
García-Hortigüela, Pilar
Fernández, Amanda
Pablos, María Victoria
Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications
description 12 Pàg. Departamento de Medio Ambiente y Agronomía​ (INIA)
author2 Agencia Estatal de Investigación (España)
author_facet Agencia Estatal de Investigación (España)
Martínez-Guitarte, José Luis
Beltrán Rodríguez, Eulalia María
González Doncel, Miguel
García-Hortigüela, Pilar
Fernández, Amanda
Pablos, María Victoria
format artículo
topic_facet Carbamazepine
Reclaimed water
Thyroid axis
X.laevis
author Martínez-Guitarte, José Luis
Beltrán Rodríguez, Eulalia María
González Doncel, Miguel
García-Hortigüela, Pilar
Fernández, Amanda
Pablos, María Victoria
author_sort Martínez-Guitarte, José Luis
title Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications
title_short Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications
title_full Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications
title_fullStr Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications
title_full_unstemmed Effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of Xenopus laevis: Gene expression modifications
title_sort effect assessment of reclaimed waters and carbamazepine exposure on the thyroid axis of xenopus laevis: gene expression modifications
publisher Elsevier
publishDate 2021-09-22
url http://hdl.handle.net/10261/280460
http://dx.doi.org/10.13039/501100011033
https://api.elsevier.com/content/abstract/scopus_id/85115797057
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