Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors
17 Pág.
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| Language: | English |
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American Society for Microbiology
2021-03-16
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| Subjects: | BSE, Prion interference, Prion propagation, Prion strain, Prion replication, Scrapie, |
| Online Access: | http://hdl.handle.net/10261/278457 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85102506681 |
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dig-inia-es-10261-2784572024-05-17T20:38:08Z Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors Espinosa Martín, Juan Carlos Andréoletti, Olivier Marín-Moreno, Alba Lugan, Séverine Aguilar-Calvo, Patricia Cassard, Hervé Lorenzo, Patricia Douet, Jean-Yves Villa Díaz, Ana Aron, Naima Prieto, Irene Huor, Alvina Torres, Juan María Ministerio de Economía y Competitividad (España) CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Espinosa Martín, Juan Carlos [0000-0002-6719-9902] Andreoletti, Olivier [0000-0002-7369-6016] Marín-Moreno, Alba [0000-0002-4023-6398] Cassard, Hervé [0000-0001-8651-6179] Prieto, Irene [0000-0001-5900-8419] Huor, Alvina [0000-0002-5598-9958] Torres, Juan María [0000-0003-0443-9232] BSE Prion interference Prion propagation Prion strain Prion replication Scrapie 17 Pág. Early studies in transgenic mouse lines have shown that the coexpression of endogenous murine prion protein (PrPC) and transgenic PrPC from another species either inhibits or allows the propagation of prions, depending on the infecting prion strain and interacting protein species. The way whereby this phenomenon, so-called "interference," is modulated remains to be determined. In this study, different transgenic mouse lines were crossbred to produce mice coexpressing bovine and porcine PrPC, bovine and murine PrPC, or murine and porcine PrPC These animals and their respective hemizygous controls were inoculated with several prion strains from different sources (cattle, mice, and pigs) to examine the effects of the simultaneous presence of PrPC from two different species. Our results indicate interference with the infection process, manifested as extended survival times and reduced attack rates. The interference with the infectious process was reduced or absent when the potentiality interfering PrPC species was efficiently converted by the inoculated agent. However, the propagation of the endogenous murine PrPSc was favored, allowing us to speculate that host-specific factors may disturb the interference caused by the coexpression of an exogenous second PrPC IMPORTANCE Prion propagation can be interfered with by the expression of a second prion protein in the host. In the present study, we investigated prion propagation in a host expressing two different prion protein genes. Our findings indicate that the ability of the second prion protein to interfere with prion propagation is related to the transmissibility of the prion in the host expressing only the interfering prion protein. The interference detected occurs in a prion strain-dependent manner. Interestingly, a bias favoring the propagation of the murine PrP allele has been observed. These results open the door to future studies in order to determine the role of host factors other than the PrP amino acid sequence in the interference in prion propagation. This work was funded by EU Projects FOOD-CT-2006-36353 (Goat-BSE) and CT2009-222887 (Priority) and by the Spanish Ministerio de Economía y Competitividad (grant AGL2016-78054-R [AEI/FEDER, UE]) and a fellowship (BES-2010-040922) to P.A.-C. A.M.-M. was supported by a fellowship from the INIA (FPI-SGIT-2015-02). Peer reviewed 2022-09-05T09:24:46Z 2022-09-05T09:24:46Z 2021-03-16 artículo http://purl.org/coar/resource_type/c_6501 mBio 12(2): 1-17 (2021) 2161-2129 http://hdl.handle.net/10261/278457 10.1128/mBio.03508-20 2150-7511 http://dx.doi.org/10.13039/501100003329 33727358 2-s2.0-85102506681 https://api.elsevier.com/content/abstract/scopus_id/85102506681 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2016-78054-R info:eu-repo/grantAgreement/MICINN//BES-2010-040922/ES/BES-2010-040922/ mBio Publisher's version https://doi.org/10.1128/mBio.03508-20 Sí open American Society for Microbiology |
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BSE Prion interference Prion propagation Prion strain Prion replication Scrapie BSE Prion interference Prion propagation Prion strain Prion replication Scrapie |
| spellingShingle |
BSE Prion interference Prion propagation Prion strain Prion replication Scrapie BSE Prion interference Prion propagation Prion strain Prion replication Scrapie Espinosa Martín, Juan Carlos Andréoletti, Olivier Marín-Moreno, Alba Lugan, Séverine Aguilar-Calvo, Patricia Cassard, Hervé Lorenzo, Patricia Douet, Jean-Yves Villa Díaz, Ana Aron, Naima Prieto, Irene Huor, Alvina Torres, Juan María Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors |
| description |
17 Pág. |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Espinosa Martín, Juan Carlos Andréoletti, Olivier Marín-Moreno, Alba Lugan, Séverine Aguilar-Calvo, Patricia Cassard, Hervé Lorenzo, Patricia Douet, Jean-Yves Villa Díaz, Ana Aron, Naima Prieto, Irene Huor, Alvina Torres, Juan María |
| format |
artículo |
| topic_facet |
BSE Prion interference Prion propagation Prion strain Prion replication Scrapie |
| author |
Espinosa Martín, Juan Carlos Andréoletti, Olivier Marín-Moreno, Alba Lugan, Séverine Aguilar-Calvo, Patricia Cassard, Hervé Lorenzo, Patricia Douet, Jean-Yves Villa Díaz, Ana Aron, Naima Prieto, Irene Huor, Alvina Torres, Juan María |
| author_sort |
Espinosa Martín, Juan Carlos |
| title |
Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors |
| title_short |
Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors |
| title_full |
Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors |
| title_fullStr |
Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors |
| title_full_unstemmed |
Allelic Interference in Prion Replication Is Modulated by the Convertibility of the Interfering PrPC and Other Host-Specific Factors |
| title_sort |
allelic interference in prion replication is modulated by the convertibility of the interfering prpc and other host-specific factors |
| publisher |
American Society for Microbiology |
| publishDate |
2021-03-16 |
| url |
http://hdl.handle.net/10261/278457 http://dx.doi.org/10.13039/501100003329 https://api.elsevier.com/content/abstract/scopus_id/85102506681 |
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