High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge

High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge

Centro de Investigación en Sanidad Animal (CISA)

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Main Authors: Cadenas-Fernández, Estefanía, Sánchez-Vizcaíno, J. M., van den Born, Erwin, Kosowska, Aleksandra, van Kilsdonk, Emma, Fernández-Pacheco, Paloma, Gallardo, Carmina, Arias, Marisa, Barasona, Jose A.
Other Authors: Ministerio de Educación, Cultura y Deporte (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2021-03-10
Subjects:African swine fever, Domestic pigs, Inactivated virus, Vaccine trial,
Online Access:http://hdl.handle.net/10261/275442
http://dx.doi.org/10.13039/501100003176
https://api.elsevier.com/content/abstract/scopus_id/85102954405
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spelling dig-inia-es-10261-2754422024-05-18T20:37:51Z High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge Cadenas-Fernández, Estefanía Sánchez-Vizcaíno, J. M. van den Born, Erwin Kosowska, Aleksandra van Kilsdonk, Emma Fernández-Pacheco, Paloma Gallardo, Carmina Arias, Marisa Barasona, Jose A. Ministerio de Educación, Cultura y Deporte (España) Cadenas-Fernández, Estefanía [0000-0001-8477-1299] Sánchez-Vizcaíno, J. M. [0000-0002-1502-8968] van den Born, Erwin [0000-0002-0341-2874] Kosowska, Aleksandra [0000-0002-0016-8765] Gallardo, Carmina [0000-0003-3293-306X] Arias, Marisa [0000-0001-6044-2968] Barasona, Jose A. [0000-0003-4066-8454] African swine fever Domestic pigs Inactivated virus Vaccine trial Centro de Investigación en Sanidad Animal (CISA) African swine fever (ASF) is currently the major concern of the global swine industry, as a consequence of which a reconsideration of the containment and prevention measures taken to date is urgently required. A great interest in developing an effective and safe vaccine against ASF virus (ASFV) infection has, therefore, recently appeared. The objective of the present study is to test an inactivated ASFV preparation under a vaccination strategy that has not previously been tested in order to improve its protective effect. The following have been considered: (i) virus inactivation by using a low binary ethyleneimine (BEI) concentration at a low temperature, (ii) the use of new and strong adjuvants; (iii) the use of very high doses (6 × 109 haemadsorption in 50% of infected cultures (HAD50)), and (iv) simultaneous double inoculation by two different routes of administration: intradermal and intramuscular. Five groups of pigs were, therefore, inoculated with BEI- Pol16/DP/OUT21 in different adjuvant formulations, twice with a 4-week interval. Six weeks later, all groups were intramuscularly challenged with 10 HAD50 of the virulent Pol16/DP/OUT21 ASFV isolate. All the animals had clinical signs and pathological findings consistent with ASF. This lack of effectiveness supports the claim that an inactivated virus strategy may not be a viable vaccine option with which to fight ASF. This research was funded by Intervet International B.V. (50–2019). E.C.-F. is a recipient of a Spanish Government-funded PhD fellowship for the Training of Future Scholars (FPU) provided by the Spanish Ministry of Education, Culture and Sports. Peer reviewed 14 Pág. 2022-07-13T12:10:18Z 2022-07-13T12:10:18Z 2021-03-10 artículo http://purl.org/coar/resource_type/c_6501 Vaccines 9(3): 242(2021) 2076-393X http://hdl.handle.net/10261/275442 10.3390/vaccines9030242 http://dx.doi.org/10.13039/501100003176 33802021 2-s2.0-85102954405 https://api.elsevier.com/content/abstract/scopus_id/85102954405 en Vaccines Publisher's version https://doi.org/10.3390/vaccines9030242 Sí open Multidisciplinary Digital Publishing Institute
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
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databasecode dig-inia-es
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region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic African swine fever
Domestic pigs
Inactivated virus
Vaccine trial
African swine fever
Domestic pigs
Inactivated virus
Vaccine trial
spellingShingle African swine fever
Domestic pigs
Inactivated virus
Vaccine trial
African swine fever
Domestic pigs
Inactivated virus
Vaccine trial
Cadenas-Fernández, Estefanía
Sánchez-Vizcaíno, J. M.
van den Born, Erwin
Kosowska, Aleksandra
van Kilsdonk, Emma
Fernández-Pacheco, Paloma
Gallardo, Carmina
Arias, Marisa
Barasona, Jose A.
High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge
description Centro de Investigación en Sanidad Animal (CISA)
author2 Ministerio de Educación, Cultura y Deporte (España)
author_facet Ministerio de Educación, Cultura y Deporte (España)
Cadenas-Fernández, Estefanía
Sánchez-Vizcaíno, J. M.
van den Born, Erwin
Kosowska, Aleksandra
van Kilsdonk, Emma
Fernández-Pacheco, Paloma
Gallardo, Carmina
Arias, Marisa
Barasona, Jose A.
format artículo
topic_facet African swine fever
Domestic pigs
Inactivated virus
Vaccine trial
author Cadenas-Fernández, Estefanía
Sánchez-Vizcaíno, J. M.
van den Born, Erwin
Kosowska, Aleksandra
van Kilsdonk, Emma
Fernández-Pacheco, Paloma
Gallardo, Carmina
Arias, Marisa
Barasona, Jose A.
author_sort Cadenas-Fernández, Estefanía
title High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge
title_short High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge
title_full High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge
title_fullStr High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge
title_full_unstemmed High Doses of Inactivated African Swine Fever Virus Are Safe, but Do Not Confer Protection against a Virulent Challenge
title_sort high doses of inactivated african swine fever virus are safe, but do not confer protection against a virulent challenge
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021-03-10
url http://hdl.handle.net/10261/275442
http://dx.doi.org/10.13039/501100003176
https://api.elsevier.com/content/abstract/scopus_id/85102954405
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