Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites

Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites

12 Pág.

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Main Authors: Hernández Moreno, David, Blázquez, María, Navas Antón, José María, Fernández-Cruz, M. L.
Other Authors: European Commission
Format: artículo biblioteca
Language:English
Published: Elsevier 2022-01
Subjects:Aquatic, Biocide, Cytotoxicity, Degradation product, In vivo, Screening,
Online Access:http://hdl.handle.net/10261/273898
http://dx.doi.org/10.13039/501100000780
https://api.elsevier.com/content/abstract/scopus_id/85119926487
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spelling dig-inia-es-10261-2738982024-05-19T20:38:10Z Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites Hernández Moreno, David Blázquez, María Navas Antón, José María Fernández-Cruz, M. L. European Commission Hernández Moreno, David [0000-0002-1189-9299] Blázquez, María [0000-0002-0139-1643] Navas Antón, José María [0000-0002-7644-8499] Fernández-Cruz, M. L. [0000-0001-5988-1939] Aquatic Biocide Cytotoxicity Degradation product In vivo Screening 12 Pág. Biocidal substances and their environmental relevant metabolites are highly toxic for fish. However, an important scarcity of toxicity data for metabolites is recognised. This article provides new data about the toxicity to fish of these compounds and evaluates the potential use of fish cell lines as screening tools to assess the acute toxicity of these compounds in fish. To this aim, acute toxicity of 7 substances was tested in Oncorhynchus mykiss (OECD TG203) and cytotoxicity of 16 substances was assessed in fish cell lines from two species; Poeciliopsis lucida (PLHC-1) and O. mykiss (RTH-149, RTG-2 and RTgill-W1) performing three cytotoxicity tests: Alamar-Blue, 5-carboxyfluorescein diacetate, acetoxymethyl ester and Neutral Red Uptake. Additionally, in vitro and in vivo data from the LIFE-COMBASE database were included in a dataset finally comprising 33 biocides and 14 metabolites. Hazard data were categorized into 4 toxicity groups, according to the intervals established in Regulation (EC) 1272/2008. Finally, the Spearman correlation test was performed and coincidences between in vitro-in vivo data established. In vitro and in vivo results revealed a high positive correlation, with a complete coincidence for 56.5% of the substances, a 2% of false positives (non-toxic in vivo) and a 13% of false negatives (toxic in vivo) for the 4 toxicity categories. However, when results were grouped in toxic or non-toxic coincidence was obtained for 85% of the substances. In conclusion, although fish denote a greater sensitivity, the use of at least two fish cell lines and three cytotoxicity endpoints appear to be valid approaches for fish acute toxicity screening of biocides and their metabolites. This work was supported by LIFE-COMBASE project (LIFE15 ENV/ES/000416). Peer reviewed 2022-06-29T08:04:09Z 2022-06-29T08:04:09Z 2022-01 artículo http://purl.org/coar/resource_type/c_6501 Aquatic Toxicology 242: 106020 (2022) 0166-445X http://hdl.handle.net/10261/273898 10.1016/j.aquatox.2021.106020 1879-1514 http://dx.doi.org/10.13039/501100000780 34844051 2-s2.0-85119926487 https://api.elsevier.com/content/abstract/scopus_id/85119926487 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/LIFE15 ENV/ES/000416 Aquatic toxicology (Amsterdam, Netherlands) Postprint https://doi.org/10.1016/j.aquatox.2021.106020 Sí open Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic Aquatic
Biocide
Cytotoxicity
Degradation product
In vivo
Screening
Aquatic
Biocide
Cytotoxicity
Degradation product
In vivo
Screening
spellingShingle Aquatic
Biocide
Cytotoxicity
Degradation product
In vivo
Screening
Aquatic
Biocide
Cytotoxicity
Degradation product
In vivo
Screening
Hernández Moreno, David
Blázquez, María
Navas Antón, José María
Fernández-Cruz, M. L.
Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites
description 12 Pág.
author2 European Commission
author_facet European Commission
Hernández Moreno, David
Blázquez, María
Navas Antón, José María
Fernández-Cruz, M. L.
format artículo
topic_facet Aquatic
Biocide
Cytotoxicity
Degradation product
In vivo
Screening
author Hernández Moreno, David
Blázquez, María
Navas Antón, José María
Fernández-Cruz, M. L.
author_sort Hernández Moreno, David
title Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites
title_short Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites
title_full Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites
title_fullStr Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites
title_full_unstemmed Fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites
title_sort fish cell lines as screening tools to predict acute toxicity to fish of biocidal active substances and their relevant environmental metabolites
publisher Elsevier
publishDate 2022-01
url http://hdl.handle.net/10261/273898
http://dx.doi.org/10.13039/501100000780
https://api.elsevier.com/content/abstract/scopus_id/85119926487
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