Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality?
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John Wiley & Sons
2022-04-25
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| Subjects: | AI doses, Boar sperm, Liquid storage, Transcripts, |
| Online Access: | http://hdl.handle.net/10261/270764 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85129186257 |
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dig-inia-es-10261-2707642024-05-17T20:35:34Z Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality? Castany Quintana, Marina Gardela, Jaume Ruiz-Conca, Mateo López-Béjar, Manel Martinez, Cristina A. Rodriguez-Martinez, Heriberto Álvarez-Rodríguez, Manuel Ministerio de Ciencia e Innovación (España) Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Gardela, Jaume [0000-0001-7524-2088] Ruiz-Conca, Mateo [0000-0001-7092-7993] López-Béjar, Manel [0000-0001-9490-6126] Martinez, Cristina A. [0000-0001-6811-0191] Rodriguez-Martinez, Heriberto [0000-0002-5194-2124] Álvarez-Rodríguez, Manuel [0000-0003-0120-354X] AI doses Boar sperm Liquid storage Transcripts 4 Pág. Artificial insemination (AI) for pigs relies on liquid storage of extended semen at 17°C, which preserves sperm quality and ensures its fertilizing capacity. Routine quality controls include the evaluation of sperm motility, viability and capacitation status. The physiological functions of all these features depend on transmembrane aquaporins (AQPs), proteins playing key roles in osmoadaptation. In this study, we made a relative quantification, using RT-qPCR, of the mRNA of several sperm AQPs in AI-liquid semen doses before and after a 48-hr incubation period, aiming to determine possible quantitative compromising expression changes during the process that could serve as a diagnostic tool. Our results showed a decrease in classical sperm motility variables (total and progressive motility and velocity) and sperm viability after 48-hr storage, whereas capacitation status increased overtime. mRNA expression increased in the orthodox AQP4 and AQP6 after 48-hr incubation, relative to control (0 hr) and 24-hr time-points. Moreover, mRNA expression of aquaglyceroporins AQP3, AQP7 and AQP10 was higher after 48-hr incubation, confirmed by AQP7-protein validation using Western blot. Our results indicate that expression levels of AQPs-mRNA can change in ejaculated pig spermatozoa under conditions of ex-vivo incubation that could modify sperm homeostasis, suggesting it could eventually become a relevant molecular biomarker to assess the efficiency of liquid storage of pig semen. This research was funded by the Research Council FORMAS, Stockholm (Projects 2017- 00946 and 2019- 00288), and by the Grant PID2019- 108320RJ- I00 and IJCI- 2015- 24380 funded by MCIN/AEI/10.13039/501100011033 (Spain) and FEDER funds (EU). Peer reviewed 2022-05-27T10:40:51Z 2022-05-27T10:40:51Z 2022-04-25 artículo http://purl.org/coar/resource_type/c_6501 Reproduction in Domestic Animals .0: 1-4(2022) 0936-6768 http://hdl.handle.net/10261/270764 10.1111/rda.14134 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 35467055 2-s2.0-85129186257 https://api.elsevier.com/content/abstract/scopus_id/85129186257 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# 2017‐00946/ Research Council FORMAS, Stockholm 2019‐00288/ Research Council FORMAS, Stockholm info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-108320RJ-I00/ES/MODULACION DE LA HOMEOSTASIS DEL TRACTO GENITAL DE LA HEMBRA POR EXOSOMAS SEMINALES: ¿MECANISMO ESENCIAL PARA LA FERTILIDAD?/ info:eu-repo/grantAgreement/MINECO//IJCI-2015-24380/ES/IJCI-2015-24380/ Reproduction in domestic animals = Zuchthygiene Publisher's version https://doi.org/10.1111/rda.14134 Sí open John Wiley & Sons |
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AI doses Boar sperm Liquid storage Transcripts AI doses Boar sperm Liquid storage Transcripts |
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AI doses Boar sperm Liquid storage Transcripts AI doses Boar sperm Liquid storage Transcripts Castany Quintana, Marina Gardela, Jaume Ruiz-Conca, Mateo López-Béjar, Manel Martinez, Cristina A. Rodriguez-Martinez, Heriberto Álvarez-Rodríguez, Manuel Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality? |
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4 Pág. |
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Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Castany Quintana, Marina Gardela, Jaume Ruiz-Conca, Mateo López-Béjar, Manel Martinez, Cristina A. Rodriguez-Martinez, Heriberto Álvarez-Rodríguez, Manuel |
| format |
artículo |
| topic_facet |
AI doses Boar sperm Liquid storage Transcripts |
| author |
Castany Quintana, Marina Gardela, Jaume Ruiz-Conca, Mateo López-Béjar, Manel Martinez, Cristina A. Rodriguez-Martinez, Heriberto Álvarez-Rodríguez, Manuel |
| author_sort |
Castany Quintana, Marina |
| title |
Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality? |
| title_short |
Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality? |
| title_full |
Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality? |
| title_fullStr |
Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality? |
| title_full_unstemmed |
Changes in aquaporins mRNA expression and liquid storage at 17°C: A potential biomarker of boar sperm quality? |
| title_sort |
changes in aquaporins mrna expression and liquid storage at 17°c: a potential biomarker of boar sperm quality? |
| publisher |
John Wiley & Sons |
| publishDate |
2022-04-25 |
| url |
http://hdl.handle.net/10261/270764 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85129186257 |
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