Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat
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| Language: | English |
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Public Library of Science
2022-04-13
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| Subjects: | Allergens, Carrier Proteins, Electrophoresis, Polyacrylamide Gel, Lipids, Molecular Docking Simulation, Plant Proteins, Proteins, Triticum, |
| Online Access: | http://hdl.handle.net/10261/269977 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85128332433 |
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dig-inia-es-10261-2699772024-05-14T20:40:24Z Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat Missaoui, Khawla Gonzalez-Klein, Zulema Jemli, Sonia Garrido-Arandía, María Díaz-Perales, Araceli Tome-Amat, Jaime Brini, Faiçal Ministerio de Ciencia e Innovación (España) Agencia Estatal de Investigación (España) Comunidad de Madrid Ministère de l’Enseignement Supérieur et de la Recherche Scientifique (Tunisie) Gonzalez-Klein, Zulema [0000-0003-3762-3781] Jemli, Sonia [0000-0001-6265-581X] Garrido-Arandía, María [0000-0001-6114-5754] Díaz-Perales, Araceli [0000-0002-1093-3627] Tome-Amat, Jaime [0000-0003-4442-3649] Brini, Faiçal [0000-0002-8435-381X] Allergens Carrier Proteins Electrophoresis Polyacrylamide Gel Lipids Molecular Docking Simulation Plant Proteins Proteins Triticum 17 Pág. Non-specific lipid transfer proteins (nsLTPs) are small, cysteine-rich proteins, a part of the pathogenesis-related protein family, and numerous of them act as positive regulators during plant disease resistance, growth, and reproduction. These proteins are involved also in the intracellular transfer of lipids, as well as in plant immune responses. Besides their differences in sequences, they show similar features in their structure. However, they show distinct lipid-binding specificities signifying their various biological roles that dictate further structural study. This study reports the identification, in silico characterization and purification of a novel member of the nsLTP2 protein family from durum wheat, TdLTP2. It was generated and purified using the combination of gel filtration chromatography and reverse-phase high-performance liquid chromatography (RP-HPLC). Its identity was detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and mass spectrometry (MALDI-TOF). TdLTP2 had been expressed in different stress to detect its localization; therefore, fluor-immunolocalization studies accomplished this data. In this approach, to assess the allergenicity of TdLTP2, thirty patients with baker's asthma were enrolled and ELISA to detect the presence of specific IgE antibodies tested their sera. Moreover, the lipid-binding properties of TdLTP2 were examined in vitro and validated using a molecular docking study. In summary, our results demonstrate a new addition of member in plant nsLTPs family, TdLTP2, which can develop a better understanding about its biological functions and shed light on future applications. This work was funded by the Spanish Ministry of Science and Innovation through the project LISENTRA, granted by the Spanish Research State Agency (PID2020-113629RBI00/AEI/10.13039/501100011033), the Comunidad de Madrid (FOODAL-CM: S2018/BAA4574) Spain, and a grant from the Ministry of Higher Education, and Scientific Research of Tunisia, and a grant from. There was no additional external funding received for this study Peer reviewed 2022-05-19T12:15:36Z 2022-05-19T12:15:36Z 2022-04-13 artículo http://purl.org/coar/resource_type/c_6501 PLoS ONE 17(4): e0266971(2022) 1932-6203 http://hdl.handle.net/10261/269977 10.1371/journal.pone.0266971 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 35417502 2-s2.0-85128332433 https://api.elsevier.com/content/abstract/scopus_id/85128332433 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/Ministerio de Ciencia e Innovación/PID2020-113629RBI00 info:eu-repo/grantAgreement/AEI/10.13039/501100011033 FOODAL-CM: S2018/BAA4574/Comunidad de Madrid PloS one Publisher's version https://doi.org/10.1371/journal.pone.0266971 Sí open Public Library of Science |
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Allergens Carrier Proteins Electrophoresis Polyacrylamide Gel Lipids Molecular Docking Simulation Plant Proteins Proteins Triticum Allergens Carrier Proteins Electrophoresis Polyacrylamide Gel Lipids Molecular Docking Simulation Plant Proteins Proteins Triticum |
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Allergens Carrier Proteins Electrophoresis Polyacrylamide Gel Lipids Molecular Docking Simulation Plant Proteins Proteins Triticum Allergens Carrier Proteins Electrophoresis Polyacrylamide Gel Lipids Molecular Docking Simulation Plant Proteins Proteins Triticum Missaoui, Khawla Gonzalez-Klein, Zulema Jemli, Sonia Garrido-Arandía, María Díaz-Perales, Araceli Tome-Amat, Jaime Brini, Faiçal Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat |
| description |
17 Pág. |
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Ministerio de Ciencia e Innovación (España) |
| author_facet |
Ministerio de Ciencia e Innovación (España) Missaoui, Khawla Gonzalez-Klein, Zulema Jemli, Sonia Garrido-Arandía, María Díaz-Perales, Araceli Tome-Amat, Jaime Brini, Faiçal |
| format |
artículo |
| topic_facet |
Allergens Carrier Proteins Electrophoresis Polyacrylamide Gel Lipids Molecular Docking Simulation Plant Proteins Proteins Triticum |
| author |
Missaoui, Khawla Gonzalez-Klein, Zulema Jemli, Sonia Garrido-Arandía, María Díaz-Perales, Araceli Tome-Amat, Jaime Brini, Faiçal |
| author_sort |
Missaoui, Khawla |
| title |
Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat |
| title_short |
Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat |
| title_full |
Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat |
| title_fullStr |
Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat |
| title_full_unstemmed |
Identification and molecular characterization of a novel non-specific lipid transfer protein (TdLTP2) from durum wheat |
| title_sort |
identification and molecular characterization of a novel non-specific lipid transfer protein (tdltp2) from durum wheat |
| publisher |
Public Library of Science |
| publishDate |
2022-04-13 |
| url |
http://hdl.handle.net/10261/269977 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100011033 https://api.elsevier.com/content/abstract/scopus_id/85128332433 |
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