Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos

Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos

Departamento de Reproducción Animal​ (INIA)

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Bibliographic Details
Main Authors: González Herrero, Raquel, Pericuesta Camacho, Eva, Gutiérrez-Adán, Alfonso, Sjunnesson, Ylva C B
Other Authors: Swedish Research Council
Format: artículo biblioteca
Language:English
Published: Elsevier 2020-03-01
Subjects:ACTH, Cortisol, In vitro culture, Pig, qRT-PCR,
Online Access:http://hdl.handle.net/10261/268973
http://dx.doi.org/10.13039/501100004837
https://api.elsevier.com/content/abstract/scopus_id/85098685014
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spelling dig-inia-es-10261-2689732024-05-14T20:38:40Z Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos González Herrero, Raquel Pericuesta Camacho, Eva Gutiérrez-Adán, Alfonso Sjunnesson, Ylva C B Swedish Research Council Ministerio de Ciencia e Innovación (España) Swedish Farmers’ Foundation Pericuesta Camacho, Eva [0000-0001-9387-3270] Gutiérrez-Adán, Alfonso [0000-0001-9893-9179] Sjunnesson, Ylva C B [0000-0003-4500-5123] ACTH Cortisol In vitro culture Pig qRT-PCR Departamento de Reproducción Animal​ (INIA) Early embryonic development may be affected by adrenal hyperactivity in stressful situations which may lead to endocrine changes in the embryo environment. A sensitive period in porcine embryo development is the 4-cell stage when the embryo genome activation occurs. A mixed in vivo-in vitro system was implemented to test whether an altered milieu around this stage could affect embryo development and blastocyst quality in the porcine model. After in vitro maturation and fertilisation, presumptive zygotes were exposed for 24 h to plasma collected after ovulation from adrenocorticotropic hormone (ACTH)-treated, non-ACTH-treated sows; and, medium without plasma, supplemented with bovine serum albumin. Subsequently, embryo development and differences in gene expression were tested among treatments. Cleavage and blastocyst rates did not differ between treatments. Blastocyst quality by morphology assessment was similar when all the resulting blastocysts were included in the analysis. However, when only expanded blastocysts (and onwards) were included in the analysis, the blastocysts from the non-ACTH plasma group showed better quality score. Blastocyst quality by morphological assessment was not mirrored by the transcription levels of various important genes for embryo development whose gene expression profile did not significantly differ among groups. It is likely that the effect of the altered environment provided by plasma from ACTH-treated sows was too short to affect embryo development. Therefore, a brief exposure to an altered endocrine environment may not have harmful consequences for the embryo once fertilisation occurs. This work was supported by The Swedish Research Council Formas (Project number 221-2007-374 and 221-2010-549); the Spanish Ministry of Science and Innovation (Grants AGL2009-11358 and RTI2018-093548-B-I00); and a travel grant from the Swedish Farmers’ Foundation. Peer reviewed 2022-05-09T08:59:00Z 2022-05-09T08:59:00Z 2020-03-01 artículo http://purl.org/coar/resource_type/c_6501 Theriogenology 162: 15-21 (2021) 0093-691X http://hdl.handle.net/10261/268973 10.1016/j.theriogenology.2020.11.012 1879-3231 http://dx.doi.org/10.13039/501100004837 33388725 2-s2.0-85098685014 https://api.elsevier.com/content/abstract/scopus_id/85098685014 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-093548-B-I00/ES/ANALISIS FUNCIONAL DE ISOFORMAS DE ARNM RELACIONADAS CON DESARROLLO EMBRIONARIO Y FERTILIDAD/ info:eu-repo/grantAgreement/MICINN//AGL2009-11358/ES/Factores Del Desarrollo Preimplantacional Que Condicionan La Salud Y Fertilidad En El Animal Adulto: Efecto De La Seleccion Espermatica, La Nutricion Embrionaria Y Las Diferencias De Genero/ Swedish Research Council Formas/ Project number 221-2007-374 and 221-2010-549 Theriogenology Publisher's version https://doi.org/10.1016/j.theriogenology.2020.11.012 Sí open Elsevier
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic ACTH
Cortisol
In vitro culture
Pig
qRT-PCR
ACTH
Cortisol
In vitro culture
Pig
qRT-PCR
spellingShingle ACTH
Cortisol
In vitro culture
Pig
qRT-PCR
ACTH
Cortisol
In vitro culture
Pig
qRT-PCR
González Herrero, Raquel
Pericuesta Camacho, Eva
Gutiérrez-Adán, Alfonso
Sjunnesson, Ylva C B
Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos
description Departamento de Reproducción Animal​ (INIA)
author2 Swedish Research Council
author_facet Swedish Research Council
González Herrero, Raquel
Pericuesta Camacho, Eva
Gutiérrez-Adán, Alfonso
Sjunnesson, Ylva C B
format artículo
topic_facet ACTH
Cortisol
In vitro culture
Pig
qRT-PCR
author González Herrero, Raquel
Pericuesta Camacho, Eva
Gutiérrez-Adán, Alfonso
Sjunnesson, Ylva C B
author_sort González Herrero, Raquel
title Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos
title_short Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos
title_full Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos
title_fullStr Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos
title_full_unstemmed Effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos
title_sort effect of an altered hormonal environment by blood plasma collected after adrenocorticotropic administration on embryo development and gene expression in porcine embryos
publisher Elsevier
publishDate 2020-03-01
url http://hdl.handle.net/10261/268973
http://dx.doi.org/10.13039/501100004837
https://api.elsevier.com/content/abstract/scopus_id/85098685014
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AT pericuestacamachoeva effectofanalteredhormonalenvironmentbybloodplasmacollectedafteradrenocorticotropicadministrationonembryodevelopmentandgeneexpressioninporcineembryos
AT gutierrezadanalfonso effectofanalteredhormonalenvironmentbybloodplasmacollectedafteradrenocorticotropicadministrationonembryodevelopmentandgeneexpressioninporcineembryos
AT sjunnessonylvacb effectofanalteredhormonalenvironmentbybloodplasmacollectedafteradrenocorticotropicadministrationonembryodevelopmentandgeneexpressioninporcineembryos
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