Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge

Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge

Centro de Investigación en Sanidad Animal (CISA)

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Main Authors: Rojas, José Manuel, Barba-Moreno, Diego, Avia, Miguel, Sevilla, Noemí, Martín, Verónica
Other Authors: Ministerio de Ciencia e Innovación (España)
Format: artículo biblioteca
Language:English
Published: Frontiers Media 2021-03-10
Subjects:IFNAR (−/−) mice, Orbivirus, T cell, Cytotoxic T lymphocytes, Vaccine,
Online Access:http://hdl.handle.net/10261/268889
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100004837
https://api.elsevier.com/content/abstract/scopus_id/85103067332
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spelling dig-inia-es-10261-2688892024-05-15T20:39:04Z Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge Rojas, José Manuel Barba-Moreno, Diego Avia, Miguel Sevilla, Noemí Martín, Verónica Ministerio de Ciencia e Innovación (España) European Commission Comunidad de Madrid Ministerio de Economía y Competitividad (España) Rojas, José Manuel [0000-0002-4055-3967] Avia, Miguel [0000-0001-7661-4779] Sevilla, Noemí [0000-0003-0118-0376] IFNAR (−/−) mice Orbivirus T cell Cytotoxic T lymphocytes Vaccine Centro de Investigación en Sanidad Animal (CISA) Bluetongue virus (BTV) is the causative agent of a disease that affects domestic and wild ruminants and leads to critical economic losses. BTV is an arbovirus from the Reoviridae family that is typically transmitted by the bite of infected Culicoides midges. BTV possesses multiple serotypes (up to 28 have been described), and immunity to one serotype offers little cross-protection to other serotypes. The design of vaccines that provide protection across multiple serotypes is therefore highly desirable to control this disease. We previously reported that a recombinant replication-defective human adenovirus serotype 5 (Ad5) that expresses the VP7 inner core protein of BTV serotype 8 (Ad5VP7-8) induced T-cell responses and provided protection. In the present work, we evaluated as BTV vaccine the combination of Ad5VP7-8 with another recombinant Ad5 that expresses the outer core protein VP2 from BTV-1 (Ad5VP2-1). The combination of Ad5VP2-1 and Ad5VP7-8 protected against homologous BTV challenge (BTV-1 and BTV-8) and partially against heterologous BTV-4 in a murine model. Cross-reactive anti-BTV immunoglobulin G (IgG) were detected in immunized animals, but no significant titers of neutralizing antibodies were elicited. The Ad5VP7-8 immunization induced T-cell responses that recognized all three serotypes tested in this study and primed cytotoxic T lymphocytes specific for VP7. This study further confirms that targeting antigenic determinant shared by several BTV serotypes using cellular immunity could help develop multiserotype BTV vaccines. This work was funded by grants AGL2015-64290R and from the Ministerio de Ciencia (Spain), grant VetBioNet INFRAIA-731014 from the EU-H2020, and grant S2018/BAA-4370-PLATESA2 from Comunidad de Madrid (Fondo Europeo de Desarrollo Regional, FEDER). MA was funded by an FPI grant (BES-2013-066406). Peer reviewed 15 Pág. 2022-05-06T10:31:43Z 2022-05-06T10:31:43Z 2021-03-10 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Veterinary Science 8: 645561 (2021) http://hdl.handle.net/10261/268889 10.3389/fvets.2021.645561 2297-1769 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100012818 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100004837 33778041 2-s2.0-85103067332 https://api.elsevier.com/content/abstract/scopus_id/85103067332 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/Ministerio de Ciencia//AGL2015-64290R info:eu-repo/grantAgreement/EH(2020)/NFRAIA-731014 S2018/BAA-4370-PLATESA2/CM info:eu-repo/grantAgreement/MINECO//BES-2013-066406/ES/BES-2013-066406/ Frontiers in veterinary science Publisher's version https://doi.org/10.3389/fvets.2021.645561 Sí open Frontiers Media
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic IFNAR (−/−) mice
Orbivirus
T cell
Cytotoxic T lymphocytes
Vaccine
IFNAR (−/−) mice
Orbivirus
T cell
Cytotoxic T lymphocytes
Vaccine
spellingShingle IFNAR (−/−) mice
Orbivirus
T cell
Cytotoxic T lymphocytes
Vaccine
IFNAR (−/−) mice
Orbivirus
T cell
Cytotoxic T lymphocytes
Vaccine
Rojas, José Manuel
Barba-Moreno, Diego
Avia, Miguel
Sevilla, Noemí
Martín, Verónica
Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge
description Centro de Investigación en Sanidad Animal (CISA)
author2 Ministerio de Ciencia e Innovación (España)
author_facet Ministerio de Ciencia e Innovación (España)
Rojas, José Manuel
Barba-Moreno, Diego
Avia, Miguel
Sevilla, Noemí
Martín, Verónica
format artículo
topic_facet IFNAR (−/−) mice
Orbivirus
T cell
Cytotoxic T lymphocytes
Vaccine
author Rojas, José Manuel
Barba-Moreno, Diego
Avia, Miguel
Sevilla, Noemí
Martín, Verónica
author_sort Rojas, José Manuel
title Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge
title_short Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge
title_full Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge
title_fullStr Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge
title_full_unstemmed Vaccination With Recombinant Adenoviruses Expressing the Bluetongue Virus Subunits VP7 and VP2 Provides Protection Against Heterologous Virus Challenge
title_sort vaccination with recombinant adenoviruses expressing the bluetongue virus subunits vp7 and vp2 provides protection against heterologous virus challenge
publisher Frontiers Media
publishDate 2021-03-10
url http://hdl.handle.net/10261/268889
http://dx.doi.org/10.13039/501100003329
http://dx.doi.org/10.13039/100012818
http://dx.doi.org/10.13039/501100000780
http://dx.doi.org/10.13039/501100004837
https://api.elsevier.com/content/abstract/scopus_id/85103067332
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