Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates
Departamento de Reproducción Animal (INIA)
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| Format: | artículo biblioteca |
| Language: | English |
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Frontiers Media
2021-06-15
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| Subjects: | Assisted reproductive technologies, Conceptus elongation, Developmental biology, Embryo quality, Embryo transfer, Lineage markers, Post-hatching embryo culture, Viability, |
| Online Access: | http://hdl.handle.net/10261/266892 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000781 https://api.elsevier.com/content/abstract/scopus_id/85109000587 |
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Assisted reproductive technologies Conceptus elongation Developmental biology Embryo quality Embryo transfer Lineage markers Post-hatching embryo culture Viability Assisted reproductive technologies Conceptus elongation Developmental biology Embryo quality Embryo transfer Lineage markers Post-hatching embryo culture Viability |
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Assisted reproductive technologies Conceptus elongation Developmental biology Embryo quality Embryo transfer Lineage markers Post-hatching embryo culture Viability Assisted reproductive technologies Conceptus elongation Developmental biology Embryo quality Embryo transfer Lineage markers Post-hatching embryo culture Viability Pérez-Gómez, Alba González-Brusi, Leopoldo Bermejo Álvarez, Pablo Ramos Ibeas, Priscila Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates |
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Departamento de Reproducción Animal (INIA) |
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European Research Council |
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European Research Council Pérez-Gómez, Alba González-Brusi, Leopoldo Bermejo Álvarez, Pablo Ramos Ibeas, Priscila |
| format |
artículo |
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Assisted reproductive technologies Conceptus elongation Developmental biology Embryo quality Embryo transfer Lineage markers Post-hatching embryo culture Viability |
| author |
Pérez-Gómez, Alba González-Brusi, Leopoldo Bermejo Álvarez, Pablo Ramos Ibeas, Priscila |
| author_sort |
Pérez-Gómez, Alba |
| title |
Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates |
| title_short |
Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates |
| title_full |
Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates |
| title_fullStr |
Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates |
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Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates |
| title_sort |
lineage differentiation markers as a proxy for embryo viability in farm ungulates |
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Frontiers Media |
| publishDate |
2021-06-15 |
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http://hdl.handle.net/10261/266892 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000781 https://api.elsevier.com/content/abstract/scopus_id/85109000587 |
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AT perezgomezalba lineagedifferentiationmarkersasaproxyforembryoviabilityinfarmungulates AT gonzalezbrusileopoldo lineagedifferentiationmarkersasaproxyforembryoviabilityinfarmungulates AT bermejoalvarezpablo lineagedifferentiationmarkersasaproxyforembryoviabilityinfarmungulates AT ramosibeaspriscila lineagedifferentiationmarkersasaproxyforembryoviabilityinfarmungulates |
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1802819611443331072 |
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dig-inia-es-10261-2668922024-05-16T20:36:04Z Lineage Differentiation Markers as a Proxy for Embryo Viability in Farm Ungulates Pérez-Gómez, Alba González-Brusi, Leopoldo Bermejo Álvarez, Pablo Ramos Ibeas, Priscila European Research Council Ministerio de Economía y Competitividad (España) Gonzalez-Brusi, L [0000-0002-6371-6423] Bermejo Álvarez, Pablo [0000-0001-9907-2626] Ramos Ibeas, Priscila [0000-0003-4578-0189] Bermejo Álvarez, Pablo [0000-0001-9907-2626] Assisted reproductive technologies Conceptus elongation Developmental biology Embryo quality Embryo transfer Lineage markers Post-hatching embryo culture Viability Departamento de Reproducción Animal (INIA) Embryonic losses constitute a major burden for reproductive efficiency of farm animals. Pregnancy losses in ungulate species, which include cattle, pigs, sheep and goats, majorly occur during the second week of gestation, when the embryo experiences a series of cell differentiation, proliferation, and migration processes encompassed under the term conceptus elongation. Conceptus elongation takes place following blastocyst hatching and involves a massive proliferation of the extraembryonic membranes trophoblast and hypoblast, and the formation of flat embryonic disc derived from the epiblast, which ultimately gastrulates generating the three germ layers. This process occurs prior to implantation and it is exclusive from ungulates, as embryos from other mammalian species such as rodents or humans implant right after hatching. The critical differences in embryo development between ungulates and mice, the most studied mammalian model, have precluded the identification of the genes governing lineage differentiation in livestock species. Furthermore, conceptus elongation has not been recapitulated in vitro, hindering the study of these cellular events. Luckily, recent advances on transcriptomics, genome modification and post-hatching in vitro culture are shedding light into this largely unknown developmental window, uncovering possible molecular markers to determine embryo quality. In this review, we summarize the events occurring during ungulate pre-implantation development, highlighting recent findings which reveal that several dogmas in Developmental Biology established by knock-out murine models do not hold true for other mammals, including humans and farm animals. The developmental failures associated to in vitro produced embryos in farm animals are also discussed together with Developmental Biology tools to assess embryo quality, including molecular markers to assess proper lineage commitment and a post-hatching in vitro culture system able to directly determine developmental potential circumventing the need of experimental animals. Research on conceptus elongation carried out by the authors and the publication costs of this review were funded by the projects StG-757886-ELONGAN from the European Research Council and AGL2017-58739-R from the Spanish Ministry of Economy and Competitiveness (MINECO). Images shown on Figure 2 were taken on an equipment funded by the project ECQ2018-005184-P from MINECO. PR-I is funded by a Ramón y Cajal Contract from MINECO (RYC2018-025666-I). The authors thank the slaughterhouses Transformación Ganadera de Leganés SA, Matadero Madrid-Norte SA, and Matadero Mondejano SL (especially to Reyes Prieto Cabañas) for gently providing the ovaries required for research on ungulate lineage differentiation markers. Peer reviewed 13 Pág. 2022-04-12T11:24:56Z 2022-04-12T11:24:56Z 2021-06-15 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Veterinary Science 8: 680539 (2021) http://hdl.handle.net/10261/266892 10.3389/fvets.2021.680539 2297-1769 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000781 34212020 2-s2.0-85109000587 https://api.elsevier.com/content/abstract/scopus_id/85109000587 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/EC/StG-757886-ELONGAN info:eu-repo/grantAgreement/MINECO//AGL2017-58739-R info:eu-repo/grantAgreement/MINECO/Contrato Ramón y Cajal/ECQ2018-005184-P Frontiers in veterinary science Publisher's version https://doi.org/10.3389/fvets.2021.680539 Sí open Frontiers Media |