Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)

Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)

This article belongs to the Special Issue West Nile Virus Infection.

Saved in:
Bibliographic Details
Main Authors: Gamino, Virginia, Pérez-Ramírez, Elisa, Gutiérrez-Guzmán, Ana Valeria, Sotelo, Elena, Llorente, Francisco, Jiménez-Clavero, Miguel Ángel, Höfle, Ursula
Other Authors: Junta de Comunidades de Castilla-La Mancha
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2021
Subjects:West Nile virus lineage 1, Inflammatory reaction, Pathogenesis, Antigen distribution, Neurovirulence, Red-legged partridge,
Online Access:http://hdl.handle.net/10261/259613
http://dx.doi.org/10.13039/501100011698
http://dx.doi.org/10.13039/501100003339
Tags: Add Tag
No Tags, Be the first to tag this record!
id dig-inia-es-10261-259613
record_format koha
spelling dig-inia-es-10261-2596132022-03-11T15:32:25Z Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa) Gamino, Virginia Pérez-Ramírez, Elisa Gutiérrez-Guzmán, Ana Valeria Sotelo, Elena Llorente, Francisco Jiménez-Clavero, Miguel Ángel Höfle, Ursula Junta de Comunidades de Castilla-La Mancha CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Consejo Superior de Investigaciones Científicas (España) West Nile virus lineage 1 Inflammatory reaction Pathogenesis Antigen distribution Neurovirulence Red-legged partridge This article belongs to the Special Issue West Nile Virus Infection. West Nile virus (WNV) is the most widespread flavivirus in the world with a wide vertebrate host range. Its geographic expansion and activity continue to increase with important human and equine outbreaks and local bird mortality. In a previous experiment, we demonstrated the susceptibility of 7-week-old red-legged partridges (Alectoris rufa) to Mediterranean WNV isolates Morocco/2003 and Spain/2007, which varied in virulence for this gallinaceous species. Here we study the pathogenesis of the infection with these two strains to explain the different course of infection and mortality. Day six post-inoculation was critical in the course of infection, with the highest viral load in tissues, the most widespread virus antigen, and more severe lesions. The most affected organs were the heart, liver, and spleen. Comparing infections with Morocco/2003 and Spain/2007, differences were observed in the viral load, virus antigen distribution, and lesion nature and severity. A more acute and marked inflammatory reaction (characterized by participation of microglia and CD3+ T cells) as well as neuronal necrosis in the brain were observed in partridges infected with Morocco/2003 as compared to those infected with Spain/2007. This suggests a higher neurovirulence of Morocco/2003, probably related to one or more specific molecular determinants of virulence different from Spain/2007. This study has been supported by the project PAC08-0296-7771 (JCCM), and from INIA-MARM funds (INIA CC08-020). Elena Sotelo was a fellow from INIA. Elisa Perez Ramirez was a fellow of the National Research Council (CSIC) and Ana Valeria Gutierrez was a fellow of the JCCM. Peer reviewed 2022-02-02T12:55:08Z 2022-02-02T12:55:08Z 2021 artículo http://purl.org/coar/resource_type/c_6501 Pathogens 10(6): 748 (2021) http://hdl.handle.net/10261/259613 10.3390/pathogens10060748 2076-0817 http://dx.doi.org/10.13039/501100011698 http://dx.doi.org/10.13039/501100003339 34199167 en Publisher's version https://doi.org/10.3390/pathogens10060748 Sí open Multidisciplinary Digital Publishing Institute
institution INIA ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-inia-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del INIA España
language English
topic West Nile virus lineage 1
Inflammatory reaction
Pathogenesis
Antigen distribution
Neurovirulence
Red-legged partridge
West Nile virus lineage 1
Inflammatory reaction
Pathogenesis
Antigen distribution
Neurovirulence
Red-legged partridge
spellingShingle West Nile virus lineage 1
Inflammatory reaction
Pathogenesis
Antigen distribution
Neurovirulence
Red-legged partridge
West Nile virus lineage 1
Inflammatory reaction
Pathogenesis
Antigen distribution
Neurovirulence
Red-legged partridge
Gamino, Virginia
Pérez-Ramírez, Elisa
Gutiérrez-Guzmán, Ana Valeria
Sotelo, Elena
Llorente, Francisco
Jiménez-Clavero, Miguel Ángel
Höfle, Ursula
Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)
description This article belongs to the Special Issue West Nile Virus Infection.
author2 Junta de Comunidades de Castilla-La Mancha
author_facet Junta de Comunidades de Castilla-La Mancha
Gamino, Virginia
Pérez-Ramírez, Elisa
Gutiérrez-Guzmán, Ana Valeria
Sotelo, Elena
Llorente, Francisco
Jiménez-Clavero, Miguel Ángel
Höfle, Ursula
format artículo
topic_facet West Nile virus lineage 1
Inflammatory reaction
Pathogenesis
Antigen distribution
Neurovirulence
Red-legged partridge
author Gamino, Virginia
Pérez-Ramírez, Elisa
Gutiérrez-Guzmán, Ana Valeria
Sotelo, Elena
Llorente, Francisco
Jiménez-Clavero, Miguel Ángel
Höfle, Ursula
author_sort Gamino, Virginia
title Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)
title_short Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)
title_full Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)
title_fullStr Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)
title_full_unstemmed Pathogenesis of two western Mediterranean west Nile virus lineage 1 isolates in experimentally infected red-legged partridges (Alectoris rufa)
title_sort pathogenesis of two western mediterranean west nile virus lineage 1 isolates in experimentally infected red-legged partridges (alectoris rufa)
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021
url http://hdl.handle.net/10261/259613
http://dx.doi.org/10.13039/501100011698
http://dx.doi.org/10.13039/501100003339
work_keys_str_mv AT gaminovirginia pathogenesisoftwowesternmediterraneanwestnileviruslineage1isolatesinexperimentallyinfectedredleggedpartridgesalectorisrufa
AT perezramirezelisa pathogenesisoftwowesternmediterraneanwestnileviruslineage1isolatesinexperimentallyinfectedredleggedpartridgesalectorisrufa
AT gutierrezguzmananavaleria pathogenesisoftwowesternmediterraneanwestnileviruslineage1isolatesinexperimentallyinfectedredleggedpartridgesalectorisrufa
AT soteloelena pathogenesisoftwowesternmediterraneanwestnileviruslineage1isolatesinexperimentallyinfectedredleggedpartridgesalectorisrufa
AT llorentefrancisco pathogenesisoftwowesternmediterraneanwestnileviruslineage1isolatesinexperimentallyinfectedredleggedpartridgesalectorisrufa
AT jimenezclaveromiguelangel pathogenesisoftwowesternmediterraneanwestnileviruslineage1isolatesinexperimentallyinfectedredleggedpartridgesalectorisrufa
AT hofleursula pathogenesisoftwowesternmediterraneanwestnileviruslineage1isolatesinexperimentallyinfectedredleggedpartridgesalectorisrufa
_version_ 1767602870865100800

Similar Items