Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae
9 páginas, 3 figuras, 3 tablas.
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| Format: | artículo biblioteca |
| Language: | English |
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American Dairy Science Association
2014-03
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| Subjects: | Ewe, Lipid, Ruminal microbiota, 16S rDNA, |
| Online Access: | http://hdl.handle.net/10261/92089 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 |
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dig-igm-es-10261-920892021-03-18T16:13:21Z Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae Castro Carrera, Tamara Toral, Pablo G. Frutos, Pilar McEwan, Neil R. Hervás, Gonzalo Abecia, Leticia Pinloche, E. Girdwood, Susan E. Belenguer, Álvaro Ministerio de Economía y Competitividad (España) Consejo Superior de Investigaciones Científicas (España) European Commission Ewe Lipid Ruminal microbiota 16S rDNA 9 páginas, 3 figuras, 3 tablas. Developing novel strategies to increase the content of bioactive unsaturated fatty acids (FA) in ruminant-derived products requires a deeper understanding of rumen biohydrogenation and bacteria involved in this process. Although high-throughput pyrosequencing may allow for a great coverage of bacterial diversity, it has hardly been used to investigate the microbiology of ruminal FA metabolism. In this experiment, 454 pyrosequencing and a molecular fingerprinting technique (terminal restriction fragment length polymorphism; T-RFLP) were used concurrently to assess the effect of diet supplementation with marine algae (MA) on the rumen bacterial community of dairy sheep. Eleven lactating ewes were divided in 2 lots and offered a total mixed ration based on alfalfa hay and concentrate (40:60), supplemented with 0 (control) or 8 (MA) g of MA/kg of dry matter. After 54 d on treatments, animals were slaughtered and samples of rumen content and fluid were collected separately for microbial analysis. Pyrosequencing yielded a greater coverage of bacterial diversity than T-RFLP and allowed the identification of low abundant populations. Conversely, both molecular approaches pointed to similar conclusions and showed that relevant changes due to MA addition were observed within the major ruminal phyla, namely Bacteroidetes, Firmicutes, and Proteobacteria. Decreases in the abundance of unclassified Bacteroidales, Porphyromonadaceae, and Ruminococcaceae and increases in as-yet uncultured species of the family Succinivibrionaceae, might be related to a potential role of these groups in different pathways of rumen FA metabolism. Diet supplementation with MA, however, had no effect on the relative abundance of Butyrivibrio and Pseudobutyrivibrio genera. In addition, results from both 454 pyrosequencing and T-RFLP indicate that the effect of MA was rather consistent in rumen content or fluid samples, despite inherent differences between these fractions in their bacterial composition. This study was supported by the Spanish Ministry of Economy and Competitiveness (Madrid, Spain; AGL2011-23700). T. Castro-Carrera was granted a predoctoral fellowship from the Spanish National Research Council (CSIC, Madrid, Spain; JAE Programme) supported by the European Social Fund (European Commission, Brussels, Belgium). Peer reviewed 2014-02-20T08:40:13Z 2014-02-20T08:40:13Z 2014-03 artículo http://purl.org/coar/resource_type/c_6501 Journal of Dairy Science 97(3) : 1661-1669 (2014) 0022-0302 http://hdl.handle.net/10261/92089 10.3168/jds.2013-7243 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 en http://dx.doi.org/10.3168/jds.2013-7243 open American Dairy Science Association |
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ES |
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Europa del Sur |
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English |
| topic |
Ewe Lipid Ruminal microbiota 16S rDNA Ewe Lipid Ruminal microbiota 16S rDNA |
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Ewe Lipid Ruminal microbiota 16S rDNA Ewe Lipid Ruminal microbiota 16S rDNA Castro Carrera, Tamara Toral, Pablo G. Frutos, Pilar McEwan, Neil R. Hervás, Gonzalo Abecia, Leticia Pinloche, E. Girdwood, Susan E. Belenguer, Álvaro Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae |
| description |
9 páginas, 3 figuras, 3 tablas. |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Castro Carrera, Tamara Toral, Pablo G. Frutos, Pilar McEwan, Neil R. Hervás, Gonzalo Abecia, Leticia Pinloche, E. Girdwood, Susan E. Belenguer, Álvaro |
| format |
artículo |
| topic_facet |
Ewe Lipid Ruminal microbiota 16S rDNA |
| author |
Castro Carrera, Tamara Toral, Pablo G. Frutos, Pilar McEwan, Neil R. Hervás, Gonzalo Abecia, Leticia Pinloche, E. Girdwood, Susan E. Belenguer, Álvaro |
| author_sort |
Castro Carrera, Tamara |
| title |
Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae |
| title_short |
Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae |
| title_full |
Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae |
| title_fullStr |
Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae |
| title_full_unstemmed |
Rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae |
| title_sort |
rumen bacterial community evaluated by 454 pyrosequencing and terminal restriction fragment length polymorphism analyses in dairy sheep fed marine algae |
| publisher |
American Dairy Science Association |
| publishDate |
2014-03 |
| url |
http://hdl.handle.net/10261/92089 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100003339 http://dx.doi.org/10.13039/501100000780 |
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