Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs
12 páginas, 4 figuras, 6 tablas.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2011
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| Subjects: | Rumen, Methanogenesis, pH, In vitro, Rumen fluid, Buffer, |
| Online Access: | http://hdl.handle.net/10261/61249 |
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dig-igm-es-10261-612492021-03-18T16:23:37Z Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs Navarro-Villa, A. O'Brien, M. López, Secundino Boland, T. M. O'Kiely, P. Rumen Methanogenesis pH In vitro Rumen fluid Buffer 12 páginas, 4 figuras, 6 tablas. The objective was to modify an in vitro rumen gas production technique (GPT) so that CH(4) production from feeds with contrasting compositions would better reflect in vivo findings. A phosphate-bicarbonate saline solution was mixed with citric acid and used as the buffer. Varying quantities (i.e., 0.3, 0.5, 0.7 g) of each of three contrasting feeds (i.e., barley grain. grass silage, barley straw) were incubated with 50 ml of varying ratios of rumen fluid to buffer (1:2, 1:4, 1:6). Effects on CH(4) output/g feed dry matter (DM) incubated (CH(4)/DMi) and/g DM disappeared (CH(4)/DMd) and other associated changes were evaluated after 24 h of incubation. The in sacco degradability of barley straw, grass silage and barley grain DM was 212, 682 and 888 g/kg, respectively. Increasing the quantity of feed incubated in vitro led to a decline in CH(4)/DMi and CH(4)/DMd and a decline in pH (P<0.001). Increasing the proportion of rumen fluid in the mixture increased CH(4)/DMi and CH(4)/DMd and reduced pH (P<0.01). Results were consistent among feeds tested. However for grass silage, apparent DM disappearance declined (P<0.001) as the quantity of feed incubated increased, an outcome associated with a simultaneous decline in pH. CH(4)/DMd is a more appropriate unit for expressing in vitro rumen CH(4) output than CH(4)/DMi and incubation of 0.3 g dried milled feed with 50 ml of rumen fluid and buffer mixture at a 1:2 ratio was a satisfactory combination with this buffer system. National Development Plan through the Research Stimulus Fund (RSF 06361) Peer reviewed 2012-11-27T10:47:52Z 2012-11-27T10:47:52Z 2011 artículo http://purl.org/coar/resource_type/c_6501 Animal Feed Science and Technology (166-67 Special Issue) : 163-174 (2011) 0377-8401 http://hdl.handle.net/10261/61249 10.1016/j.anifeedsci.2011.04.064 en http://dx.doi.org/10.1016/j.anifeedsci.2011.04.064 none Elsevier |
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biblioteca |
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Europa del Sur |
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Biblioteca del IGM España |
| language |
English |
| topic |
Rumen Methanogenesis pH In vitro Rumen fluid Buffer Rumen Methanogenesis pH In vitro Rumen fluid Buffer |
| spellingShingle |
Rumen Methanogenesis pH In vitro Rumen fluid Buffer Rumen Methanogenesis pH In vitro Rumen fluid Buffer Navarro-Villa, A. O'Brien, M. López, Secundino Boland, T. M. O'Kiely, P. Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs |
| description |
12 páginas, 4 figuras, 6 tablas. |
| format |
artículo |
| topic_facet |
Rumen Methanogenesis pH In vitro Rumen fluid Buffer |
| author |
Navarro-Villa, A. O'Brien, M. López, Secundino Boland, T. M. O'Kiely, P. |
| author_facet |
Navarro-Villa, A. O'Brien, M. López, Secundino Boland, T. M. O'Kiely, P. |
| author_sort |
Navarro-Villa, A. |
| title |
Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs |
| title_short |
Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs |
| title_full |
Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs |
| title_fullStr |
Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs |
| title_full_unstemmed |
Modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs |
| title_sort |
modifications of a gas production technique for assessing in vitro rumen methane production from feedstuffs |
| publisher |
Elsevier |
| publishDate |
2011 |
| url |
http://hdl.handle.net/10261/61249 |
| work_keys_str_mv |
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