Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak
Glaria, Idoia et al.-- 10 páginas, 5 figuras, 2 tablas.
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| Format: | artículo biblioteca |
| Language: | English |
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Elsevier
2012-03-23
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| Subjects: | Visna Maedi, Neurological outbreak, Small ruminant lentivirus, Complete genomes, LTR promoter activity, Peptide ELISA diagnosis, |
| Online Access: | http://hdl.handle.net/10261/59192 |
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dig-igm-es-10261-591922021-04-13T15:44:40Z Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak Glaria, Idoia Reina, Ramsés Ramírez, Hugo Andrés, Ximena de Crespo, Helena Jáuregui, Paula Benavides, Julio Pérez Pérez, Valentín Amorena Zabalza, Beatriz Andrés, Damián F. de Visna Maedi Neurological outbreak Small ruminant lentivirus Complete genomes LTR promoter activity Peptide ELISA diagnosis Glaria, Idoia et al.-- 10 páginas, 5 figuras, 2 tablas. An extensive outbreak characterized by the appearance of neurological symptoms in small ruminant lentivirus (SRLV) infected sheep has been identified in Spain, but the genetic characteristics of the strain involved and differential diagnostic tools for this outbreak remain unexplored. In this work, 23 Visna-affected naturally infected animals from the outbreak, 11 arthritic animals (both groups presenting anti-Visna/Maedi virus serum antibodies), and 100 seronegative animals were used. Eight of the Visna-affected animals were further studied post-mortem by immunohistochemistry. All had lesions in spinal cord, being the most affected part of the central nervous system in six of them. A representative strain of the outbreak was isolated. Together with other proviral sequences from the outbreak the virus was assigned to genotype A2/A3. In vitro culture of the isolate revealed that viral production was slow/low in fibroblast-like cells but it was high in blood monocyte-derived macrophages. The long terminal repeat (LTR) of the viral genome of this isolate lacked an U3-duplication, but its promoter activity in fibroblast-like cells was normal compared to other strains. Thus, viral production could not be inferred from the LTR promoter activity in this isolate. Analysis of the viral immunodominant epitopes among SRLV sequences of the outbreak and other known sequences allowed the design of a synthetic SU peptide ELISA that detected the Visna affected animals, representing a tool of epidemiological interest to control viral spread of this highly pathogenic strain. Spanish CICYT AGL2006-13410-006-01/GAN AGL2010-22341-C04-01 . Government of Navarra, Department of Agriculture Public University of Navarra . Spanish CSIC Peer reviewed 2012-10-30T11:42:26Z 2012-10-30T11:42:26Z 2012-03-23 artículo http://purl.org/coar/resource_type/c_6501 Veterinary Microbiology 155(2-4): 137-146 (2012) 0378-1135 http://hdl.handle.net/10261/59192 10.1016/j.vetmic.2011.08.027 en http://dx.doi.org/10.1016/j.vetmic.2011.08.027 Sí none Elsevier |
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Visna Maedi Neurological outbreak Small ruminant lentivirus Complete genomes LTR promoter activity Peptide ELISA diagnosis Visna Maedi Neurological outbreak Small ruminant lentivirus Complete genomes LTR promoter activity Peptide ELISA diagnosis |
| spellingShingle |
Visna Maedi Neurological outbreak Small ruminant lentivirus Complete genomes LTR promoter activity Peptide ELISA diagnosis Visna Maedi Neurological outbreak Small ruminant lentivirus Complete genomes LTR promoter activity Peptide ELISA diagnosis Glaria, Idoia Reina, Ramsés Ramírez, Hugo Andrés, Ximena de Crespo, Helena Jáuregui, Paula Benavides, Julio Pérez Pérez, Valentín Amorena Zabalza, Beatriz Andrés, Damián F. de Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak |
| description |
Glaria, Idoia et al.-- 10 páginas, 5 figuras, 2 tablas. |
| format |
artículo |
| topic_facet |
Visna Maedi Neurological outbreak Small ruminant lentivirus Complete genomes LTR promoter activity Peptide ELISA diagnosis |
| author |
Glaria, Idoia Reina, Ramsés Ramírez, Hugo Andrés, Ximena de Crespo, Helena Jáuregui, Paula Benavides, Julio Pérez Pérez, Valentín Amorena Zabalza, Beatriz Andrés, Damián F. de |
| author_facet |
Glaria, Idoia Reina, Ramsés Ramírez, Hugo Andrés, Ximena de Crespo, Helena Jáuregui, Paula Benavides, Julio Pérez Pérez, Valentín Amorena Zabalza, Beatriz Andrés, Damián F. de |
| author_sort |
Glaria, Idoia |
| title |
Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak |
| title_short |
Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak |
| title_full |
Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak |
| title_fullStr |
Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak |
| title_full_unstemmed |
Visna/Maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak |
| title_sort |
visna/maedi virus genetic characterization and serological diagnosis of infection in sheep from a neurological outbreak |
| publisher |
Elsevier |
| publishDate |
2012-03-23 |
| url |
http://hdl.handle.net/10261/59192 |
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