Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences
8 páginas, 6 figuras, 1 tabla.
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| Format: | artículo biblioteca |
| Language: | English |
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Cambridge University Press
2011
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| Subjects: | Dicrocoelium dendriticum, Mollusc and ant intermediate hosts, PCR diagnosis, Mitochondrial and ITS-2 squences, |
| Online Access: | http://hdl.handle.net/10261/46374 |
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dig-igm-es-10261-463742021-04-13T15:13:13Z Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences Martínez Ibeas, Ana Martínez Valladares, María González Lanza, Camino Miñambres Rodríguez, Baltasar Manga-González, M. Yolanda Dicrocoelium dendriticum Mollusc and ant intermediate hosts PCR diagnosis Mitochondrial and ITS-2 squences 8 páginas, 6 figuras, 1 tabla. The aim of this study was to develop, perfect and validate the PCR (polymerase chain reaction) technique using mitochondrial (mt) and ribosomal (ITS-2) DNA for the accurate identification of Dicrocoelium dendriticum in molluscs and ants, the first and second intermediate hosts, and their early detection. The first primers that were designed amplified a 169 pb mtDNA fragment of D. dendriticum permitted the detection of a single D. dendriticum metacercaria from the Formica rufibarbis and Formica pratensis abdomen, as well as the detection of the brainworm in the head of the ants collected in tetania. Although these primers did not amplify Dicrocoelium chinensis DNA and permitted detected D. dendriticum in the molluscs, they did not discriminate Brachylaimidae metacercariae found in the same mollusc. The PCR that was designed to amplify a 93 bp fragment of the ITS-2 is D. dendriticum specific as it did not amplify D. chinensis, Brachylaimidae and other trematodes. This technique is very sensitive since it permitted the detection of D. dendriticum in the molluscs from the first day post-infection, the brainworm in the head of the ants and only 1 D. dendriticum metacercaria from the abdomen of the ants. Both techniques are important, mainly the latter. This study was supported by the Spanish CICYT (Project AGL2007–62824). Martínez-Ibeas is supported by the ‘Castile and León’ Autonomy (Spain) and the European Social Funds (Contract for young researchers). Martínez- Valladares is supported by the Spanish National Research Council (CSIC) (JAE Doc Contract). Peer reviewed 2012-02-29T10:54:07Z 2012-02-29T10:54:07Z 2011 artículo http://purl.org/coar/resource_type/c_6501 Parasitology 138(14) : 1916-1923 (2011) 0031-1820 http://hdl.handle.net/10261/46374 10.1017/S0031182011001375 en http://dx.doi.org/10.1017/S0031182011001375 open Cambridge University Press |
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Dicrocoelium dendriticum Mollusc and ant intermediate hosts PCR diagnosis Mitochondrial and ITS-2 squences Dicrocoelium dendriticum Mollusc and ant intermediate hosts PCR diagnosis Mitochondrial and ITS-2 squences |
| spellingShingle |
Dicrocoelium dendriticum Mollusc and ant intermediate hosts PCR diagnosis Mitochondrial and ITS-2 squences Dicrocoelium dendriticum Mollusc and ant intermediate hosts PCR diagnosis Mitochondrial and ITS-2 squences Martínez Ibeas, Ana Martínez Valladares, María González Lanza, Camino Miñambres Rodríguez, Baltasar Manga-González, M. Yolanda Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences |
| description |
8 páginas, 6 figuras, 1 tabla. |
| format |
artículo |
| topic_facet |
Dicrocoelium dendriticum Mollusc and ant intermediate hosts PCR diagnosis Mitochondrial and ITS-2 squences |
| author |
Martínez Ibeas, Ana Martínez Valladares, María González Lanza, Camino Miñambres Rodríguez, Baltasar Manga-González, M. Yolanda |
| author_facet |
Martínez Ibeas, Ana Martínez Valladares, María González Lanza, Camino Miñambres Rodríguez, Baltasar Manga-González, M. Yolanda |
| author_sort |
Martínez Ibeas, Ana |
| title |
Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences |
| title_short |
Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences |
| title_full |
Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences |
| title_fullStr |
Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences |
| title_full_unstemmed |
Detection of Dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by PCR, using mitochondrial and ribosomal internal transcribed spacer (ITS-2) sequences |
| title_sort |
detection of dicrocoelium dendriticum larval stages in mollusc and ant intermediate hosts by pcr, using mitochondrial and ribosomal internal transcribed spacer (its-2) sequences |
| publisher |
Cambridge University Press |
| publishDate |
2011 |
| url |
http://hdl.handle.net/10261/46374 |
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