Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)

Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)

7 páginas, 2 figuras, 2 tablas.

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Main Authors: Martín, Alba, García-Estrada, Carlos, Melcón-Fernández, Estela, Calogero, Raffaele A., Arigoni, Maddalena, Ceciliani, Fabrizio, Lecchi, Cristina, Dehnavi, Mahsa, Giráldez, Francisco Javier, Andrés, Sonia
Other Authors: Ministerio de Ciencia e Innovación (España)
Format: Article biblioteca
Language:English
Published: Elsevier 2024-08-01
Subjects:Cytokines, Exosomes, Feed restriction, Milk, miRNA, Nutritional programming, Responsible Consumption and Production, cytokines,
Online Access:http://hdl.handle.net/10261/363451
https://api.elsevier.com/content/abstract/scopus_id/85194459060
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id dig-igm-es-10261-363451
record_format koha
institution IGM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-igm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IGM España
language English
topic Cytokines
Exosomes
Feed restriction
Milk
miRNA
Nutritional programming
Responsible Consumption and Production
cytokines
Milk
Cytokines
Exosomes
Feed restriction
Milk
miRNA
Nutritional programming
Responsible Consumption and Production
cytokines
Milk
spellingShingle Cytokines
Exosomes
Feed restriction
Milk
miRNA
Nutritional programming
Responsible Consumption and Production
cytokines
Milk
Cytokines
Exosomes
Feed restriction
Milk
miRNA
Nutritional programming
Responsible Consumption and Production
cytokines
Milk
Martín, Alba
García-Estrada, Carlos
Melcón-Fernández, Estela
Calogero, Raffaele A.
Arigoni, Maddalena
Ceciliani, Fabrizio
Lecchi, Cristina
Dehnavi, Mahsa
Giráldez, Francisco Javier
Andrés, Sonia
Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)
description 7 páginas, 2 figuras, 2 tablas.
author2 Ministerio de Ciencia e Innovación (España)
author_facet Ministerio de Ciencia e Innovación (España)
Martín, Alba
García-Estrada, Carlos
Melcón-Fernández, Estela
Calogero, Raffaele A.
Arigoni, Maddalena
Ceciliani, Fabrizio
Lecchi, Cristina
Dehnavi, Mahsa
Giráldez, Francisco Javier
Andrés, Sonia
format Article
topic_facet Cytokines
Exosomes
Feed restriction
Milk
miRNA
Nutritional programming
Responsible Consumption and Production
cytokines
Milk
author Martín, Alba
García-Estrada, Carlos
Melcón-Fernández, Estela
Calogero, Raffaele A.
Arigoni, Maddalena
Ceciliani, Fabrizio
Lecchi, Cristina
Dehnavi, Mahsa
Giráldez, Francisco Javier
Andrés, Sonia
author_sort Martín, Alba
title Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)
title_short Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)
title_full Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)
title_fullStr Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)
title_full_unstemmed Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1)
title_sort feed restriction during the suckling period of ewe assaf lambs (f0) modifies milk quality and milk exosomal mirnaome of the filial generation (f1)
publisher Elsevier
publishDate 2024-08-01
url http://hdl.handle.net/10261/363451
https://api.elsevier.com/content/abstract/scopus_id/85194459060
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spelling dig-igm-es-10261-3634512024-07-11T11:56:18Z Feed restriction during the suckling period of ewe Assaf lambs (F0) modifies milk quality and milk exosomal miRNAome of the filial generation (F1) Martín, Alba García-Estrada, Carlos Melcón-Fernández, Estela Calogero, Raffaele A. Arigoni, Maddalena Ceciliani, Fabrizio Lecchi, Cristina Dehnavi, Mahsa Giráldez, Francisco Javier Andrés, Sonia Ministerio de Ciencia e Innovación (España) European Commission Agencia Estatal de Investigación (España) Andrés, Sonia [0000-0001-5255-1582] Giráldez, Francisco Javier [0000-0002-0680-1541] Dehnavi, Mahsa [0000-0003-3972-7293] Cytokines Exosomes Feed restriction Milk miRNA Nutritional programming Responsible Consumption and Production cytokines Milk 7 páginas, 2 figuras, 2 tablas. Feed restriction during the early life of ewe lambs (F0) triggers the transfer of epigenetic marks to the next generation, thus impairing the reproductive performance of F1. However, the effects of this factor on milk production and composition, including its abundance in regulatory miRNA (many of which are contained in exosomes, small vesicles of endocytic origin that play a role in the modulation of immune response of the offspring) has not been tested so far. Therefore, in this study, the replacement ewe lambs (F0) obtained in a previous project (a group of ewes fed milk replacer ad libitum –ADL– vs. a group of ewes restricted –RES– to 62.5 % the intake level of milk replacer during the suckling period) were raised under similar post-weaning conditions and mated to obtain the progeny (F1). The F1 female lambs were also mated to obtain F2. Milk production was recorded during the peak lactation period of F1, and milk samples were obtained for each gland (udder half) separately to measure chemical composition, somatic cell counts (SCC), and bacteria counts. Moreover, exosomes were also isolated from the milk of each gland separately to obtain the miRNAome following a Next Generation Sequencing approach. No significant differences were found in either milk production of F1, chemical composition of milk (fat, protein, lactase), or bacteriology (colony forming units, CFU). However, SCC was reduced significantly in milk samples of F1-RES dairy sheep, whereas the abundance of five miRNAs was also modified. Thus, oar-miR-150, oar-miR-221, oar-miR-23a, oar-miR-27a, oar-miR-376c were all down modulated in F1-RES when compared to F1-ADL. Most of these miRNAs have been found to play a role in biological functions such as development, apoptosis, muscle differentiation, reproduction, or milk production. However, the exosomes extracted from the milk of these sheep (F1-RES) did not affect the production of IL-9 and IL-2 cytokines after in vitro culture with CaCo-2 cells. This study reveals that nutritional programming events such as feed restriction may drive the abundance of not only SCC but also some milk's bioactive components such as miRNAs, although it is not clear if these changes may modulate the immune response at the intestinal level of the offspring. This work was funded by Ministerio de Ciencia e Innovacion ´ (PID2021-126489OB-I00, MCIN/AEI/10.13039/501100011033, “FEDER, Una manera de hacer Europa”). Alba Martín gratefully acknowledges receipt of a pre-doctoral grant (PRE2019-089288) from Ministerio de Ciencia e Innovacion ´ (MCIN/AEI/10.13039/501100011033, “El FSE invierte en tu futuro”). CENSYRA is also gratefully acknowledged for milk analyses Peer reviewed 2024-07-11T11:56:17Z 2024-07-11T11:56:17Z 2024-08-01 Article Animal Feed Science and Technology, 314: 116015 (2024) 0377-8401 http://hdl.handle.net/10261/363451 10.1016/j.anifeedsci.2024.116015 2-s2.0-85194459060 https://api.elsevier.com/content/abstract/scopus_id/85194459060 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126489OB-I00/ES/PROGRAMACION NUTRICIONAL EN ETAPAS TEMPRANAS DE LA VIDA PARA MEJORAR LA EFICIENCIA ALIMENTARIA, LOS INDICES REPRODUCTIVOS Y LA CALIDAD DE LA CARNE Y LA LECHE OVINA/ info:eu-repo/grantAgreement//PRE2019-089288 Animal Feed Science and Technology Publisher's version http://dx.doi.org/doi.org/10.1016/j.anifeedsci.2024.116015 Sí open Elsevier

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