Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro

Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro

16 páginas, 7 figuras.

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Bibliographic Details
Main Authors: Criado, M., Pérez Pérez, Valentín, Arteche, Noive, Elguezabal, Natalia, Molina, Elena, Benavides, Julio, Gutiérrez-Expósito, D.
Other Authors: Agencia Estatal de Investigación (España)
Format: artículo biblioteca
Language:English
Published: BioMed Central 2023
Subjects:Neutrophils, NETs, Phagocytosis, Mycobacterium, Paratuberculosis, Cytokines, Ruminants, Responsible Consumption and Production,
Online Access:http://hdl.handle.net/10261/332796
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spelling dig-igm-es-10261-3327962023-08-08T10:56:37Z Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro Criado, M. Pérez Pérez, Valentín Arteche, Noive Elguezabal, Natalia Molina, Elena Benavides, Julio Gutiérrez-Expósito, D. Agencia Estatal de Investigación (España) Ministerio de Ciencia e Innovación (España) Arteche Villasol, Noive [0000-0001-5793-9578] Benavides, Julio [0000-0001-9706-100X] Gutiérrez Expósito, Daniel [0000-0002-7683-623X] Pérez Pérez, Valentín [0000-0003-0075-1587] Neutrophils NETs Phagocytosis Mycobacterium Paratuberculosis Cytokines Ruminants Responsible Consumption and Production 16 páginas, 7 figuras. Neutrophils constitute an essential component of the innate immune response, readily killing most bacteria through phagocytosis, degranulation, and the release of neutrophil extracellular traps (NETs) among other mecha‑ nisms. These cells play an unclear role in mycobacterial infections such as Mycobacterium avium subspecies paratuberculosis (Map), the etiological agent of paratuberculosis, and its response is particularly understudied in ruminants. Herein, a wide set of techniques were adapted, or newly developed, to study the in vitro response of caprine neu‑ trophils after Map infection. Immunofuorescence was used to demonstrate, simultaneously, chemotaxis, phagocy‑ tosis, degranulation, and NETs. The quantifcation of neutrophil phagocytic activity against Map at a 1:10 multiplicity of infection (MOI), through fow cytometry, showed values that varied from 4.54 to 5.63% of phagocyting neutrophils. By immunofuorescence, a 73.3±14.5% of the felds showed NETs, and the mean release of DNA, attributable to NETo‑ sis, calculated through a fuorometric method, was 16.2±3.5%. In addition, the RNA expression of TGF-β, TNF and IL-1β cytokines, measured through reverse transcription qPCR, was signifcantly higher in the two latter. Overall, neutrophil response was proportional to the number of bacteria. This work confrms that the simultaneous study of several neutrophil mechanisms, and the combination of diferent methodologies, are essential to reach a comprehensive understanding of neutrophil response against pathogens, demonstrates that, in vitro, caprine neutrophils display a strong innate response against Map, using their entire repertoire of efector functions, and sets the basis for further in vitro and in vivo studies on the role of neutrophils in paratuberculosis. This work was funded by the projects RTI2018-099496-B-I00 and PID2021-125807OB-C21, from the “Agencia Estatal de Investigación”, of the Spanish Ministry of Science and Innovation. Miguel Criado Boyero acknowl‑ edges the receipt of a pre-doctoral grant (PRE2019-087309) from MCIN/AEI/ https://doi.org/10.13039/501100011033 and FSE “El FSE invierte en tu futuro”. Peer reviewed 2023-08-08T10:56:37Z 2023-08-08T10:56:37Z 2023 artículo Veterinary Research, 54 (1): 61 (2023) http://hdl.handle.net/10261/332796 10.1186/s13567-023-01193-7 1297-9716 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099496-B-I00/ES/MECANISMOS DE RESISTENCIA NATURAL E INDUCIDA POR LA VACUNACION FRENTE A LA PARATUBERCULOSIS/ info:eu-repo/grantAgreement/MICINN//PID2021-125807OB-C21 info:eu-repo/grantAgreement/AEI/PRE2019-087309 Publisher's version https://dx.doi.org/10.1186/s13567-023-01193-7 Sí open BioMed Central
institution IGM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-igm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IGM España
language English
topic Neutrophils
NETs
Phagocytosis
Mycobacterium
Paratuberculosis
Cytokines
Ruminants
Responsible Consumption and Production
Neutrophils
NETs
Phagocytosis
Mycobacterium
Paratuberculosis
Cytokines
Ruminants
Responsible Consumption and Production
spellingShingle Neutrophils
NETs
Phagocytosis
Mycobacterium
Paratuberculosis
Cytokines
Ruminants
Responsible Consumption and Production
Neutrophils
NETs
Phagocytosis
Mycobacterium
Paratuberculosis
Cytokines
Ruminants
Responsible Consumption and Production
Criado, M.
Pérez Pérez, Valentín
Arteche, Noive
Elguezabal, Natalia
Molina, Elena
Benavides, Julio
Gutiérrez-Expósito, D.
Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro
description 16 páginas, 7 figuras.
author2 Agencia Estatal de Investigación (España)
author_facet Agencia Estatal de Investigación (España)
Criado, M.
Pérez Pérez, Valentín
Arteche, Noive
Elguezabal, Natalia
Molina, Elena
Benavides, Julio
Gutiérrez-Expósito, D.
format artículo
topic_facet Neutrophils
NETs
Phagocytosis
Mycobacterium
Paratuberculosis
Cytokines
Ruminants
Responsible Consumption and Production
author Criado, M.
Pérez Pérez, Valentín
Arteche, Noive
Elguezabal, Natalia
Molina, Elena
Benavides, Julio
Gutiérrez-Expósito, D.
author_sort Criado, M.
title Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro
title_short Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro
title_full Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro
title_fullStr Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro
title_full_unstemmed Evaluation of the innate immune response of caprine neutrophils against Mycobacterium avium susbspecies paratuberculosis in vitro
title_sort evaluation of the innate immune response of caprine neutrophils against mycobacterium avium susbspecies paratuberculosis in vitro
publisher BioMed Central
publishDate 2023
url http://hdl.handle.net/10261/332796
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