Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model

Arteche, Noive; Gutiérrez-Expósito, D.; Criado, M.; Benavides, Julio; Pérez Pérez, Valentín; Ministerio de Ciencia e Innovación (España)

Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model

9 páginas 2 figuras.

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Bibliographic Details
Main Authors:	Arteche, Noive, Gutiérrez-Expósito, D., Criado, M., Benavides, Julio, Pérez Pérez, Valentín
Other Authors:	Ministerio de Ciencia e Innovación (España)
Format:	artículo biblioteca
Language:	English
Published:	Multidisciplinary Digital Publishing Institute 2022
Subjects:	Vaccination, Paratuberculosis, Sheep, NETs,
Online Access:	http://hdl.handle.net/10261/281575 http://dx.doi.org/10.13039/501100014180 http://dx.doi.org/10.13039/501100004837
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spelling	dig-igm-es-10261-2815752022-11-04T17:14:43Z Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model Arteche, Noive Gutiérrez-Expósito, D. Criado, M. Benavides, Julio Pérez Pérez, Valentín Ministerio de Ciencia e Innovación (España) Junta de Castilla y León Gutiérrez Expósito, Daniel [0000-0002-7683-623X] Benavides, Julio [0000-0001-9706-100X] Pérez Pérez, Valentín [0000-0003-0075-1587] Vaccination Paratuberculosis Sheep NETs 9 páginas 2 figuras. Vaccination of domestic ruminants against paratuberculosis has been related to homologous and heterologous protective effects that have been attributed to the establishment of a trained immune response. Recent evidence suggests that neutrophils could play a role in its development. Therefore, we propose an in vitro model for the study of the effect of paratuberculosis vaccination on the release of neutrophil extracellular traps (NETs) in sheep. Ovine neutrophils were obtained from non-vaccinated (n = 5) and vaccinated sheep (n = 5) at different times post-vaccination and infected in vitro with Mycobacterium avium subsp. paratuberculosis (Map), Staphylococcus aureus (SA), and Escherichia coli (EC). NETs release was quantified by fluorimetry and visualized by immunofluorescence microscopy. Typical NETs components (DNA, neutrophil elastase, and myeloperoxidase) were visualized extracellularly in all infected neutrophils; however, no significant percentage of extracellular DNA was detected in Map-infected neutrophils compared with SA- and EC-infected. In addition, no significant effect was detected in relation to paratuberculosis vaccination. Further assays to study NETs release in ovine neutrophils are needed. Preliminary results suggest no implication of NETs formation in the early immune response after vaccination, although other neutrophil functions should be evaluated. This work was financially supported by the Spanish Ministry of Science and Innovation (projects AGL2015-66540-C2-1-R and RTI2018-099496-B-I00), and Junta de Castilla y León (project LE259P18). Noive Arteche-Villasol was the recipient of a predoctoral contract (BES-2016-076513) from the Spanish Ministry of Science and Innovation. Peer reviewed 2022-10-25T11:11:29Z 2022-10-25T11:11:29Z 2022 artículo Vaccines (Basel), 10(9): 1403 (2022) http://hdl.handle.net/10261/281575 2076-393X http://dx.doi.org/10.13039/501100014180 http://dx.doi.org/10.13039/501100004837 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2015-66540-C2-1-R/ES/RESPUESTA INMUNE Y MARCADORES DE PROTECCION ASOCIADOS A LA VACUNACION EN PARATUBERCULOSIS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099496-B-I00/ES/MECANISMOS DE RESISTENCIA NATURAL E INDUCIDA POR LA VACUNACION FRENTE A LA PARATUBERCULOSIS/ info:eu-repo/grantAgreement/AEI//BES-2016-076513 Publisher's version http://dx.doi.org/10.3390/vaccines10091403 Sí open Multidisciplinary Digital Publishing Institute
institution	IGM ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-igm-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IGM España
language	English
topic	Vaccination Paratuberculosis Sheep NETs Vaccination Paratuberculosis Sheep NETs
spellingShingle	Vaccination Paratuberculosis Sheep NETs Vaccination Paratuberculosis Sheep NETs Arteche, Noive Gutiérrez-Expósito, D. Criado, M. Benavides, Julio Pérez Pérez, Valentín Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model
description	9 páginas 2 figuras.
author2	Ministerio de Ciencia e Innovación (España)
author_facet	Ministerio de Ciencia e Innovación (España) Arteche, Noive Gutiérrez-Expósito, D. Criado, M. Benavides, Julio Pérez Pérez, Valentín
format	artículo
topic_facet	Vaccination Paratuberculosis Sheep NETs
author	Arteche, Noive Gutiérrez-Expósito, D. Criado, M. Benavides, Julio Pérez Pérez, Valentín
author_sort	Arteche, Noive
title	Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model
title_short	Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model
title_full	Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model
title_fullStr	Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model
title_full_unstemmed	Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model
title_sort	assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model
publisher	Multidisciplinary Digital Publishing Institute
publishDate	2022
url	http://hdl.handle.net/10261/281575 http://dx.doi.org/10.13039/501100014180 http://dx.doi.org/10.13039/501100004837
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Assesment of paratuberculosis vaccination effect of in vitro formation of neutrophil extracelular traps in a sheep model

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