Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis
16 páginas, 6 figuras, 3 tablas.
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| Language: | English |
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Frontiers Media
2022
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| Subjects: | Vaccination, Paratuberculosis, Tuberculosis, HIMB, Adjuvant, Cross-protection, |
| Online Access: | http://hdl.handle.net/10261/271708 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100014180 |
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Vaccination Paratuberculosis Tuberculosis HIMB Adjuvant Cross-protection Vaccination Paratuberculosis Tuberculosis HIMB Adjuvant Cross-protection |
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Vaccination Paratuberculosis Tuberculosis HIMB Adjuvant Cross-protection Vaccination Paratuberculosis Tuberculosis HIMB Adjuvant Cross-protection Arteche, Noive Gutiérrez-Expósito, D. Elguezabal, N. Sevilla, I.A. Vallejo, R. Espinosa Cerrato, José Ferreras, Mª del Carmen Benavides, Julio Pérez Pérez, Valentín Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis |
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16 páginas, 6 figuras, 3 tablas. |
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Ministerio de Ciencia e Innovación (España) |
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Ministerio de Ciencia e Innovación (España) Arteche, Noive Gutiérrez-Expósito, D. Elguezabal, N. Sevilla, I.A. Vallejo, R. Espinosa Cerrato, José Ferreras, Mª del Carmen Benavides, Julio Pérez Pérez, Valentín |
| format |
artículo |
| topic_facet |
Vaccination Paratuberculosis Tuberculosis HIMB Adjuvant Cross-protection |
| author |
Arteche, Noive Gutiérrez-Expósito, D. Elguezabal, N. Sevilla, I.A. Vallejo, R. Espinosa Cerrato, José Ferreras, Mª del Carmen Benavides, Julio Pérez Pérez, Valentín |
| author_sort |
Arteche, Noive |
| title |
Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis |
| title_short |
Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis |
| title_full |
Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis |
| title_fullStr |
Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis |
| title_full_unstemmed |
Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis |
| title_sort |
influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis |
| publisher |
Frontiers Media |
| publishDate |
2022 |
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http://hdl.handle.net/10261/271708 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100014180 |
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1777664111570583552 |
| spelling |
dig-igm-es-10261-2717082022-07-16T02:56:03Z Influence of heterologous and homologous vaccines, and their components, on the host immune response and protection against experimental caprine paratuberculosis Arteche, Noive Gutiérrez-Expósito, D. Elguezabal, N. Sevilla, I.A. Vallejo, R. Espinosa Cerrato, José Ferreras, Mª del Carmen Benavides, Julio Pérez Pérez, Valentín Ministerio de Ciencia e Innovación (España) Junta de Castilla y León CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Arteche Villasol, Noive [0000-0001-5793-9578] Gutiérrez Expósito, Daniel [0000-0002-7683-623X] Espinosa Cerrato, José [0000-0002-9036-1402] Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229] Benavides, Julio [0000-0001-9706-100X] Pérez Pérez, Valentín [0000-0003-0075-1587] Vaccination Paratuberculosis Tuberculosis HIMB Adjuvant Cross-protection 16 páginas, 6 figuras, 3 tablas. Vaccination against paratuberculosis, a chronic disease of ruminants caused by Mycobacterium avium subsp. paratuberculosis (Map), has been considered as the most effective control method. However, protection is incomplete, and the mechanisms operating in the response of the animals to vaccination are not fully understood. Therefore, this study analyzed the immune response and the effects on protection against Map infection, elicited by paratuberculosis (Silirum®) and tuberculosis (heat-inactivated M. bovis [HIMB]) vaccines and their components in a caprine experimental model. Fifty goat kids were divided into 10 groups (n = 5) according to their vaccination (Silirum®, HIMB and nonvaccinated), immunization (inactivated bacteria or adjuvant), and/or infection. Oral challenge with Map was performed 45 days postvaccination/immunization (dpv), and animals were euthanized at 190 dpv. Peripheral immune response and proportion of lymphocyte subpopulations were assessed monthly by enzyme-linked immunosorbent assay and flow cytometry analysis, respectively. Local immune response, proportion of tissue lymphocyte subpopulations, Map detection (polymerase chain reaction), and histological examination were conducted in gut-associated lymphoid tissues. All infected groups developed paratuberculosis granulomatous lesions despite vaccination or immunization. The Silirum® and HIMB-vaccinated groups showed a considerable lesion reduction consistent with a significant peripheral cellular and humoral immune response. Besides, a lower number of granulomas were observed in groups immunized with inactivated bacteria and adjuvants in comparison to nonvaccinated and infected group. However, despite not being significant, this reduction was even higher in adjuvant immunized groups, which developed milder granulomatous lesion with no detectable peripheral immune responses associated with immunization. No changes in the peripheral and local proportion of lymphocyte subsets or local immune response were detected in relation to either vaccination/immunization or infection. Despite that paratuberculosis and tuberculosis vaccination showed a partial and cross-protection against Map infection, respectively, only histological examination could assess the progression of infection in these animals. In addition, the pattern observed in the reduction of the lesions in adjuvant immunized groups suggests the possible involvement of a nonspecific immune response that reduces the development of granulomatous lesions. This work was financially supported by the Spanish Ministry of Science and Innovation (projects AGL2015- 66540-C2-1-R and RTI2018-099496-B-I00), Junta de Castilla y León (project LE259P18), and National Institute for Agronomic Research (project RTA 2017- 00089-00-00). NA-V was the recipient of a predoctoral contract (BES-2016-076513) from the Spanish Ministry of Science and Innovation and DG-E and JE of a postdoctoral contract from the Ministry of Science and Innovation (grants nos. FJCI-2017-32020 and FJC2019-042422-I respectively). Peer reviewed 2022-06-06T12:13:23Z 2022-06-06T12:13:23Z 2022 artículo http://purl.org/coar/resource_type/c_6501 Frontiers in Veterinary Science, 8: 744568 (2022) http://hdl.handle.net/10261/271708 2297-1769 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100014180 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO//AGL2015-66540-C2-1-R/ES/RESPUESTA INMUNE Y MARCADORES DE PROTECCION ASOCIADOS A LA VACUNACION EN PARATUBERCULOSIS/ info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-099496-B-I00/ES/MECANISMOS DE RESISTENCIA NATURAL E INDUCIDA POR LA VACUNACION FRENTE A LA PARATUBERCULOSIS/ info:eu-repo/grantAgreement/INIA//RTA 2017-00089-00-00 info:eu-repo/grantAgreement/MCINN//FJCI-2017-32020 info:eu-repo/grantAgreement/MCINN//FJC2019-042422-I Publisher's version http://dx.doi.org/10.3389/fvets.2021.744568 Sí open Frontiers Media |