Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts
32 páginas, 5 figuras.
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| Format: | artículo biblioteca |
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Elsevier BV
2021
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| Subjects: | Echinococcus granulosus, Local immune response, Cytokines, Polyparasit, Ism, Fasciola hepatica, Immunohistochemistry, |
| Online Access: | http://hdl.handle.net/10261/244052 |
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dig-igm-es-10261-2440522022-03-11T08:24:55Z Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts Jiménez, M. Hidalgo, C. Stoore, C. Pereira, I. Hernández, M. Sáenz, L. Benavides, Julio Ferreras, Mª del Carmen Royo, M. Paredes, R. Universidad Andrés Bello Benavides, Julio [0000-0001-9706-100X] Ferreras Estrada, Mª del Carmen [0000-0003-1996-2229] Echinococcus granulosus Local immune response Cytokines Polyparasit Ism Fasciola hepatica Immunohistochemistry 32 páginas, 5 figuras. Cystic echinococcosis is a widespread zoonosis caused by the larval stage of the tapeworm Echinococcus gran-ulosus. In intermediary hosts, two types of echinococcal cysts can be found: fertile, which produce protoscoleces, the infective form of the parasite to dogs; and infertile, that do not present protoscoleces and therefore are not able to continue with the parasite life cycle. The adventitial layer, the local immune response against the cyst, plays an important role in cyst fertility. Grazing cattle can often feature Fasciola hepatica co-infection, a parasite known to modulate the host systemic immune response. In this work the cellular Th1/Th2 immune profiles were evaluated in the adventitial layer of fertile and non-fertile cysts with and without co-infection with Fasciola hepatica. Measuring with immunohistochemistry and qPCR in adventitial layer, we report that non-fertile cysts present higher levels of Th1 cytokines (IFN-γ (P<0.0001) and TNF-α (P<0.05)), and fertile cysts have higher levels of Th2 cytokines (IL-4 (P<0.001)). Co-infection with Fasciola hepatica is associated with a decrease in the expression of IL-4 (P<0.05) and an increase in the expression of IFN-γ (P<0.0001) in the adventitial layer of fertile cysts. Non-fertile cysts were associated with higher levels of Th1 cytokines in the adventitial layer, with IFN-γ expression enhanced by F. hepatica co-infection (P<0.0001), confirming that polyparasitism should be considered in the treatment and control of naturally infected cattle. The author(s) declared no potential conflicts of interest with respect to the research, authorship, and/or publication of this article. We thank all the people who indirectly participated in this work, including vol-unteers, professionals and technical staff of the Escuela de Medicina Veterinaria, Facultad de Ciencias de la Vida, Universidad Andres Bello. This work was supported by FONDECYT-ANID, grant number 1190817 (RP); Universidad Andres Bello, internal project DI-1388-16/I (MJ). 2021-06-18T10:10:02Z 2021-06-18T10:10:02Z 2021 2021-06-18T10:10:02Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.vetpar.2021.109343 issn: 0304-4017 Veterinary Parasitology 290: 109343 (2021) http://hdl.handle.net/10261/244052 10.1016/j.vetpar.2021.109343 Postprint http://dx.doi.org/10.1016/j.vetpar.2021.109343 Sí open Elsevier BV |
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Echinococcus granulosus Local immune response Cytokines Polyparasit Ism Fasciola hepatica Immunohistochemistry Echinococcus granulosus Local immune response Cytokines Polyparasit Ism Fasciola hepatica Immunohistochemistry |
| spellingShingle |
Echinococcus granulosus Local immune response Cytokines Polyparasit Ism Fasciola hepatica Immunohistochemistry Echinococcus granulosus Local immune response Cytokines Polyparasit Ism Fasciola hepatica Immunohistochemistry Jiménez, M. Hidalgo, C. Stoore, C. Pereira, I. Hernández, M. Sáenz, L. Benavides, Julio Ferreras, Mª del Carmen Royo, M. Paredes, R. Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts |
| description |
32 páginas, 5 figuras. |
| author2 |
Universidad Andrés Bello |
| author_facet |
Universidad Andrés Bello Jiménez, M. Hidalgo, C. Stoore, C. Pereira, I. Hernández, M. Sáenz, L. Benavides, Julio Ferreras, Mª del Carmen Royo, M. Paredes, R. |
| format |
artículo |
| topic_facet |
Echinococcus granulosus Local immune response Cytokines Polyparasit Ism Fasciola hepatica Immunohistochemistry |
| author |
Jiménez, M. Hidalgo, C. Stoore, C. Pereira, I. Hernández, M. Sáenz, L. Benavides, Julio Ferreras, Mª del Carmen Royo, M. Paredes, R. |
| author_sort |
Jiménez, M. |
| title |
Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts |
| title_short |
Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts |
| title_full |
Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts |
| title_fullStr |
Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts |
| title_full_unstemmed |
Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts |
| title_sort |
fasciola hepatica co-infection enhances th1 immune response in the adventitial layer of non-fertile echinococcus granulosus cysts |
| publisher |
Elsevier BV |
| publishDate |
2021 |
| url |
http://hdl.handle.net/10261/244052 |
| work_keys_str_mv |
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1777664090509934592 |