Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping

Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping

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© 2021 by the authors.

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Main Authors: Ramírez, Hugo, Echeverría, Irache, Benito, Alfredo A., Glaria, Idoia, Benavides, Julio, Pérez, Valentín, Andrés, Damián F. de, Reina, Ramsés
Other Authors: Ministerio de Ciencia, Innovación y Universidades (España)
Format: artículo biblioteca
Language:English
Published: Multidisciplinary Digital Publishing Institute 2021-01-19
Subjects:Small ruminant lentiviruses, TMEM154, ELISA, PCR,
Online Access:http://hdl.handle.net/10261/227927
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100004435
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spelling dig-igm-es-10261-2279272021-12-27T15:43:09Z Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping Ramírez, Hugo Echeverría, Irache Benito, Alfredo A. Glaria, Idoia Benavides, Julio Pérez, Valentín Andrés, Damián F. de Reina, Ramsés Ministerio de Ciencia, Innovación y Universidades (España) Agencia Estatal de Investigación (España) CSIC - Unidad de Recursos de Información Científica para la Investigación (URICI) Universidad de Navarra Small ruminant lentiviruses TMEM154 ELISA PCR © 2021 by the authors. Small ruminant lentiviruses (SRLV) cause an incurable multiorganic disease widely spread in sheep and goats that disturbs animal welfare and production. In the absence of a vaccine, control measures have been traditionally based on early diagnosis and breeding with virus-inactivated colostrum with segregation of seropositive animals. However, antigenic heterogeneity, poor antibody production due to low viral load, and single strain design of most available ELISA, pose a threat to SRLV diagnosis. Genome-wide association studies have described TMEM154 E35K polymorphism as a good genetic marker for selection of resistant animals in some American and European breeds. In this study, a multitargeted serological and virological screening of more than 500 animals from four different breeds (latxa, raza Navarra, assaf, and churra) attending to SRLV infection status was performed. Then, animals were genotyped to characterize TMEM154 E35K polymorphism. ELISA procedures, individually considered, only identified a proportion of the seropositive animals, and PCR detected a fraction of seronegative animals, globally offering different animal classifications according to SRLV infection status. TMEM154 allele frequency differed substantially among breeds and a positive association between seroprevalence and TMEM154 genotype was found only in one breed. Selection based on TMEM154 may be suitable for specific ovine breeds or SRLV strains, however generalization to the whole SRLV genetic spectrum, ovine breeds, or epidemiological situation may need further validation. This research was funded by Spanish Ministry of Science, Innovation, and Universities, grant number RTI2018-096172-B-C31. The APC was funded by the CSIC Open Access Publication Support Initiative through its Unit of Information Resources for Research (URICI). I.E. was funded by Universidad Pública de Navarra. Hugo Ramírez was financially supported by the “Programa de apoyos para la superación del personal académico (PASPA)” scholarship from DGAPA-UNAM. Peer reviewed 2021-01-28T14:18:10Z 2021-01-28T14:18:10Z 2021-01-19 2021-01-28T14:18:11Z artículo http://purl.org/coar/resource_type/c_6501 Pathogens 10(1): 83 (2021) http://hdl.handle.net/10261/227927 10.3390/pathogens10010083 2076-0817 http://dx.doi.org/10.13039/501100011033 http://dx.doi.org/10.13039/501100004435 33478070 en #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTI2018-096172-B-C31 RTI2018-096172-B-C31/AEI/10.13039/501100011033 Publisher’s version https://doi.org/10.3390/pathogens10010083 Sí open Multidisciplinary Digital Publishing Institute
institution IGM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-igm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IGM España
language English
topic Small ruminant lentiviruses
TMEM154
ELISA
PCR
Small ruminant lentiviruses
TMEM154
ELISA
PCR
spellingShingle Small ruminant lentiviruses
TMEM154
ELISA
PCR
Small ruminant lentiviruses
TMEM154
ELISA
PCR
Ramírez, Hugo
Echeverría, Irache
Benito, Alfredo A.
Glaria, Idoia
Benavides, Julio
Pérez, Valentín
Andrés, Damián F. de
Reina, Ramsés
Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
description © 2021 by the authors.
author2 Ministerio de Ciencia, Innovación y Universidades (España)
author_facet Ministerio de Ciencia, Innovación y Universidades (España)
Ramírez, Hugo
Echeverría, Irache
Benito, Alfredo A.
Glaria, Idoia
Benavides, Julio
Pérez, Valentín
Andrés, Damián F. de
Reina, Ramsés
format artículo
topic_facet Small ruminant lentiviruses
TMEM154
ELISA
PCR
author Ramírez, Hugo
Echeverría, Irache
Benito, Alfredo A.
Glaria, Idoia
Benavides, Julio
Pérez, Valentín
Andrés, Damián F. de
Reina, Ramsés
author_sort Ramírez, Hugo
title Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
title_short Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
title_full Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
title_fullStr Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
title_full_unstemmed Accurate Diagnosis of Small Ruminant Lentivirus Infection Is Needed for Selection of Resistant Sheep through TMEM154 E35K Genotyping
title_sort accurate diagnosis of small ruminant lentivirus infection is needed for selection of resistant sheep through tmem154 e35k genotyping
publisher Multidisciplinary Digital Publishing Institute
publishDate 2021-01-19
url http://hdl.handle.net/10261/227927
http://dx.doi.org/10.13039/501100011033
http://dx.doi.org/10.13039/501100004435
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