Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum
12 páginas, 5 figuras, 2 tablas.
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| Format: | artículo biblioteca |
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Elsevier
2020
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| Subjects: | Neospora caninum, Sheep, Recrudescence, Immune response, Immunohistochemistry, Cytokines, |
| Online Access: | http://hdl.handle.net/10261/225425 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 |
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Neospora caninum Sheep Recrudescence Immune response Immunohistochemistry Cytokines Neospora caninum Sheep Recrudescence Immune response Immunohistochemistry Cytokines |
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Neospora caninum Sheep Recrudescence Immune response Immunohistochemistry Cytokines Neospora caninum Sheep Recrudescence Immune response Immunohistochemistry Cytokines Gutiérrez-Expósito, D. González-Warleta, M. Espinosa Cerrato, José Vallejo García, Raquel Castro-Hermida, J. A. Calvo, C. Ferreras, Mª del Carmen Pérez Pérez, Valentín Pérez Pérez, Valentín Benavides, Julio Mezo, Mercedes Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum |
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12 páginas, 5 figuras, 2 tablas. |
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Ministerio de Ciencia, Innovación y Universidades (España) |
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Ministerio de Ciencia, Innovación y Universidades (España) Gutiérrez-Expósito, D. González-Warleta, M. Espinosa Cerrato, José Vallejo García, Raquel Castro-Hermida, J. A. Calvo, C. Ferreras, Mª del Carmen Pérez Pérez, Valentín Pérez Pérez, Valentín Benavides, Julio Mezo, Mercedes |
| format |
artículo |
| topic_facet |
Neospora caninum Sheep Recrudescence Immune response Immunohistochemistry Cytokines |
| author |
Gutiérrez-Expósito, D. González-Warleta, M. Espinosa Cerrato, José Vallejo García, Raquel Castro-Hermida, J. A. Calvo, C. Ferreras, Mª del Carmen Pérez Pérez, Valentín Pérez Pérez, Valentín Benavides, Julio Mezo, Mercedes |
| author_sort |
Gutiérrez-Expósito, D. |
| title |
Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum |
| title_short |
Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum |
| title_full |
Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum |
| title_fullStr |
Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum |
| title_full_unstemmed |
Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum |
| title_sort |
maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of neospora caninum |
| publisher |
Elsevier |
| publishDate |
2020 |
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http://hdl.handle.net/10261/225425 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 |
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AT gutierrezexpositod maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT gonzalezwarletam maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT espinosacerratojose maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT vallejogarciaraquel maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT castrohermidaja maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT calvoc maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT ferrerasmadelcarmen maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT perezperezvalentin maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT perezperezvalentin maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT benavidesjulio maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum AT mezomercedes maternalimmuneresponseintheplacentaofsheepduringrecrudescenceofnaturalcongenitalinfectionofneosporacaninum |
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1777664079868985344 |
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dig-igm-es-10261-2254252021-04-13T15:28:32Z Maternal immune response in the placenta of sheep during recrudescence of natural congenital infection of Neospora caninum Gutiérrez-Expósito, D. González-Warleta, M. Espinosa Cerrato, José Vallejo García, Raquel Castro-Hermida, J. A. Calvo, C. Ferreras, Mª del Carmen Pérez Pérez, Valentín Pérez Pérez, Valentín Benavides, Julio Mezo, Mercedes Ministerio de Ciencia, Innovación y Universidades (España) European Commission Ministerio de Ciencia e Innovación (España) Benavides, Julio [0000-0001-9706-100X] Pérez Pérez, Valentín [0000-0003-0075-1587] Neospora caninum Sheep Recrudescence Immune response Immunohistochemistry Cytokines 12 páginas, 5 figuras, 2 tablas. In order to gain further insight into the pathogenesis and transmission of ovine neosporosis, the serological response of 13 naturally infected pregnant sheep was monitored. All sheep were euthanized upon the detection of a sharp increase in the level of specific antibodies against N. caninum in order to study the maternal immune response after the recrudescence of a chronic infection. Ten sheep were euthanized between 84 and 118 days of gestation, whereas the three remaining and three control not infected, pregnant sheep were euthanized at 135 days of gestation after no sharp increase in antibodies was detected. Vertical transmission was confirmed in 11 sheep by detection of N. caninum-DNA in at least one fetus, confirming recrudescence. Not all of fetuses showed pathologic microscopic lesions, however, multifocal non-purulent encephalitis was the main finding. Furthermore, nine out of the 11 vertical transmission positive sheep had lesions in placentomes (mainly multifocal necrotic foci), and the parasite was detected in eight out of 11 placentas by PCR and/or immunohistochemestry. The placentomes from sheep that suffered recrudescence showed an increased number of T lymphocytes CD3+ (CD4/CD8 < 1) and macrophages (MHC-II+), assessed by immunohistochemestry, together with an upregulation of IFN-γ, IL-10, IL-4, TNFα, IL-2 and IL-18. IL-17 was only upregulated in the three infected sheep that did not have a sharp increase in antibody levels. In the sheep that showed fetal death at the time of euthanasia (n = 3) the placental microscopic lesions were more severe, the inflammatory infiltrate was higher, and the upregulation of cytokines was greater than in those sheep carrying viable fetuses. This study suggests that, similarly to bovine neosporosis, the time of gestation when recrudescence occurs determines the viability of the fetuses and, thus, seems to be related to the severity of lesions and immune response in the placenta. These results suggest that there might be a correlation, either as cause or as a consequence, between protection against vertical transmission of the parasite and a milder maternal serological response together with a high level of transcription of IL-17 in the placenta. The present study was financed by the Ministerio de Ciencia, Innovacion y Universidades of Spain (INIA project RTA2014-00013) and the European Regional Development Fund. Daniel Gutiérrez-Exposito ´ is the recipient of a postdoctoral contract from the Ministry of Science and Innovation (grant no. FJCI-2017-32020). We wish to acknowledge María del Carmen Carro, Xavier Cortizo, María Teresa Carro and Marta Silva for their excellent technical assistance. Finally, we wish to acknowledge Dr. Luis Miguel Ortega-Mora (SALUVET group) for kindly providing the in-house polyclonal antibody against N. caninum. 2020-12-22T12:01:16Z 2020-12-22T12:01:16Z 2020 2020-12-22T12:01:16Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1016/j.vetpar.2020.109204 issn: 1873-2550 Veterinary Parasitology 285: 109204 (2020) http://hdl.handle.net/10261/225425 10.1016/j.vetpar.2020.109204 http://dx.doi.org/10.13039/501100004837 http://dx.doi.org/10.13039/501100000780 #PLACEHOLDER_PARENT_METADATA_VALUE# #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/RTA2014-00013 info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/FJCI-2017-32020 http://dx.doi.org/10.1016/j.vetpar.2020.109204 Sí open Elsevier |