RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway
17 páginas, 5 tablas, 5 figuras.
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Nature Publishing Group
2019
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| Subjects: | Monocyte-Derived Macrophages, Johnes-Disease, Cell-Proliferation, Differential Gene, Crohn’s disease, Dairy-Cattle, Expression, Bovis, Association, Culture, |
| Online Access: | http://hdl.handle.net/10261/212445 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100011941 |
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Monocyte-Derived Macrophages Johnes-Disease Cell-Proliferation Differential Gene Crohn’s disease Dairy-Cattle Expression Bovis Association Culture Monocyte-Derived Macrophages Johnes-Disease Cell-Proliferation Differential Gene Crohn’s disease Dairy-Cattle Expression Bovis Association Culture |
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Monocyte-Derived Macrophages Johnes-Disease Cell-Proliferation Differential Gene Crohn’s disease Dairy-Cattle Expression Bovis Association Culture Monocyte-Derived Macrophages Johnes-Disease Cell-Proliferation Differential Gene Crohn’s disease Dairy-Cattle Expression Bovis Association Culture Alonso Hearn, M. Canive, M. Blanco-Vázquez, C. Torremocha, R. Balseiro, Ana Amado, J. Varela-Martínez, Endika Ramos, R. Jugo, B. M. Casais, R. RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway |
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17 páginas, 5 tablas, 5 figuras. |
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Ministerio de Ciencia, Innovación y Universidades (España) |
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Ministerio de Ciencia, Innovación y Universidades (España) Alonso Hearn, M. Canive, M. Blanco-Vázquez, C. Torremocha, R. Balseiro, Ana Amado, J. Varela-Martínez, Endika Ramos, R. Jugo, B. M. Casais, R. |
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artículo |
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Monocyte-Derived Macrophages Johnes-Disease Cell-Proliferation Differential Gene Crohn’s disease Dairy-Cattle Expression Bovis Association Culture |
| author |
Alonso Hearn, M. Canive, M. Blanco-Vázquez, C. Torremocha, R. Balseiro, Ana Amado, J. Varela-Martínez, Endika Ramos, R. Jugo, B. M. Casais, R. |
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Alonso Hearn, M. |
| title |
RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway |
| title_short |
RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway |
| title_full |
RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway |
| title_fullStr |
RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway |
| title_full_unstemmed |
RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway |
| title_sort |
rna-seq analysis of ileocecal valve and peripheral blood from holstein cattle infected with mycobacterium avium subsp. paratuberculosis revealed dysregulation of the cxcl8/il8 signaling pathway |
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Nature Publishing Group |
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2019 |
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http://hdl.handle.net/10261/212445 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100011941 |
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dig-igm-es-10261-2124452021-12-28T15:55:14Z RNA-Seq analysis of ileocecal valve and peripheral blood from Holstein cattle infected with Mycobacterium avium subsp. paratuberculosis revealed dysregulation of the CXCL8/IL8 signaling pathway Alonso Hearn, M. Canive, M. Blanco-Vázquez, C. Torremocha, R. Balseiro, Ana Amado, J. Varela-Martínez, Endika Ramos, R. Jugo, B. M. Casais, R. Ministerio de Ciencia, Innovación y Universidades (España) Ministerio de Economía y Competitividad (España) CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) European Commission Principado de Asturias Principado de Asturias Monocyte-Derived Macrophages Johnes-Disease Cell-Proliferation Differential Gene Crohn’s disease Dairy-Cattle Expression Bovis Association Culture 17 páginas, 5 tablas, 5 figuras. Paratuberculosis is chronic granulomatous enteritis of ruminants caused by Mycobacterium avium subsp. paratuberculosis (MAP). Whole RNA-sequencing (RNA-Seq) is a promising source of novel biomarkers for early MAP infection and disease progression in cattle. Since the blood transcriptome is widely used as a source of biomarkers, we analyzed whether it recapitulates, at least in part, the transcriptome of the ileocecal valve (ICV), the primary site of MAP colonization. Total RNA was prepared from peripheral blood (PB) and ICV samples, and RNA-Seq was used to compare gene expression between animals with focal or diffuse histopathological lesions in gut tissues versus control animals with no detectable signs of infection. Our results demonstrated both shared, and PB and ICV-specific gene expression in response to a natural MAP infection. As expected, the number of differentially expressed (DE) genes was larger in the ICV than in the PB samples. Among the DE genes in the PB and ICV samples, there were some common genes irrespective of the type of lesion including the C-X-C motif chemokine ligand 8 (CXCL8/IL8), apolipoprotein L (APOLD1), and the interferon inducible protein 27 (IF127). The biological processes (BP) enriched in the PB gene expression profiles from the cows with diffuse lesions included the killing of cells of other organism, defense response, immune response and the regulation of neutrophil chemotaxis. Two of these BP, the defense and immune response, were also enriched in the ICV from the cows with diffuse lesions. Metabolic analysis of the DE genes revealed that the N-glycan biosynthesis, bile secretion, one-carbon pool by folate and purine metabolism were significantly enriched in the ICV from the cows with focal lesions. In the ICV from cows with diffuse lesions; the valine, leucine and isoleucine degradation route, purine metabolism, vitamin digestion and absorption and the cholesterol routes were enriched. Some of the identified DE genes, BP and metabolic pathways will be studied further to develop novel diagnostic tools, vaccines and immunotherapeutics. This work was supported by grants from the Instituto Nacional de Investigacion y Tecnologia Agraria y Alimentaria (INIA) and by European Funds for Regional Development (FEDER) (INIA RTA2014-00009-C02 and RTA2018-094192). The study is partially funded by the Principado de Asturias (PCTI 2018-2020, GRUPIN: IDI2018-000237). Maria Canive and Cristina Blanco-Vazquez are recipients of INIA fellowships. The authors thank ASTEGA Veterinary services for their assistance on sample collection. We are also grateful to Kyle Hearn for the careful editing of the manuscript. 2020-05-28T10:11:50Z 2020-05-28T10:11:50Z 2019 2020-05-28T10:11:50Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1038/s41598-019-51328-0 e-issn: 2045-2322 Scientific Reports 9: 14845 (2019) http://hdl.handle.net/10261/212445 10.1038/s41598-019-51328-0 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/100007652 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/100011941 31619718 #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/RTA2018-094192 Publisher's version http://dx.doi.org/10.1038/s41598-019-51328-0 Sí open Nature Publishing Group |