Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation
Parameters such as pathogen dose and inoculation route are paramount in animal models when studying disease pathogenesis. Here, clinical findings, including foetal mortality, parasite transmission rates and lesion severity, and immune responses were evaluated in Asturiana pregnant heifers at day 110 of gestation challenged with a virulent (Nc-Spain7) Neospora caninum isolate. Four different doses of parasite tachyzoites were inoculated intravenously (IV1, 107 parasites, n = 6; IV2, 105, n = 6; IV3, 103, n = 6; and IV4, 102, n = 5), and the subcutaneous (SC) inoculation route was also assessed for the dose of 105 tachyzoites (SC, n = 6). In addition, a control group (n = 4 pregnant heifers) was evaluated. Foetal death was observed in all infected groups from 25 to 62 days post-infection, varying with the dose (IV1:4/6, IV2:3/6; IV4:2/5, IV3:1/6), and was three times less frequently associated with the SC route than IV inoculation (1/6 vs. 3/6). A dose-dependent effect for parasite loads in placental and foetal brain tissues was also detected. After SC challenge, a reduced number of tachyzoites were able to reach foetal brain tissues, and no lesions were observed. In calves, specific IgG responses in precolostral sera were mainly associated with high-dose groups (IV1 [100.0%] and IV2 [66.7%]), and cerebral parasite DNA detection was scarce (3/18). In dams, IFN-γ production and the dynamics of anti-N. caninum IgG antibodies varied with the dose, and the cell-mediated immune response was also found to be route-dependent. Our results confirm the influence of parasite dose and inoculation route on the outcome and dynamics of bovine neosporosis at mid-gestation.
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BioMed Central
2019-09-23
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Online Access: | http://hdl.handle.net/10261/191808 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002911 http://dx.doi.org/10.13039/100007652 |
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dig-igm-es-10261-1918082021-12-28T16:03:34Z Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation Vázquez, Patricia Osoro, Koldo Fernández, Miguel Román-Trufero, Alicia Regidor-Cerrillo, Javier Jiménez-Pelayo, Laura García-Sánchez, Marta Rojo-Montejo, Silvia Benavides, Julio Horcajo, Pilar Ortega-Mora, Luis M. Ministerio de Economía y Competitividad (España) Universidad Complutense de Madrid CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA) Parameters such as pathogen dose and inoculation route are paramount in animal models when studying disease pathogenesis. Here, clinical findings, including foetal mortality, parasite transmission rates and lesion severity, and immune responses were evaluated in Asturiana pregnant heifers at day 110 of gestation challenged with a virulent (Nc-Spain7) Neospora caninum isolate. Four different doses of parasite tachyzoites were inoculated intravenously (IV1, 107 parasites, n = 6; IV2, 105, n = 6; IV3, 103, n = 6; and IV4, 102, n = 5), and the subcutaneous (SC) inoculation route was also assessed for the dose of 105 tachyzoites (SC, n = 6). In addition, a control group (n = 4 pregnant heifers) was evaluated. Foetal death was observed in all infected groups from 25 to 62 days post-infection, varying with the dose (IV1:4/6, IV2:3/6; IV4:2/5, IV3:1/6), and was three times less frequently associated with the SC route than IV inoculation (1/6 vs. 3/6). A dose-dependent effect for parasite loads in placental and foetal brain tissues was also detected. After SC challenge, a reduced number of tachyzoites were able to reach foetal brain tissues, and no lesions were observed. In calves, specific IgG responses in precolostral sera were mainly associated with high-dose groups (IV1 [100.0%] and IV2 [66.7%]), and cerebral parasite DNA detection was scarce (3/18). In dams, IFN-γ production and the dynamics of anti-N. caninum IgG antibodies varied with the dose, and the cell-mediated immune response was also found to be route-dependent. Our results confirm the influence of parasite dose and inoculation route on the outcome and dynamics of bovine neosporosis at mid-gestation. Patricia Vázquez had a Juan de la Cierva-Formación post-doctoral contract (FJCI-2014-20982) from the Spanish Ministry of Economy and Competitiveness (MINECO). Laura Jiménez-Pelayo was founded by UCM-Santander and Marta García-Sánchez was founded by MINECO (BES-2014-070723). Alicia Román-Trufero was supported by a 4-year FPI-INIA fellowship from the Spanish National Institute for Agriculture and Food Research and Technology (INIA). Peer reviewed 2019-09-29T05:27:39Z 2019-09-29T05:27:39Z 2019-09-23 2019-09-29T05:27:40Z artículo http://purl.org/coar/resource_type/c_6501 Veterinary Research 50(1): 68 (2019) 0928-4249 http://hdl.handle.net/10261/191808 10.1186/s13567-019-0686-3 1297-9716 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002911 http://dx.doi.org/10.13039/100007652 31547877 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/FJCI-2014-20982 Publisher's version https://doi.org/10.1186/s13567-019-0686-3 Sí open BioMed Central |
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Parameters such as pathogen dose and inoculation route are paramount in animal models when studying disease pathogenesis. Here, clinical findings, including foetal mortality, parasite transmission rates and lesion severity, and immune responses were evaluated in Asturiana pregnant heifers at day 110 of gestation challenged with a virulent (Nc-Spain7) Neospora caninum isolate. Four different doses of parasite tachyzoites were inoculated intravenously (IV1, 107 parasites, n = 6; IV2, 105, n = 6; IV3, 103, n = 6; and IV4, 102, n = 5), and the subcutaneous (SC) inoculation route was also assessed for the dose of 105 tachyzoites (SC, n = 6). In addition, a control group (n = 4 pregnant heifers) was evaluated. Foetal death was observed in all infected groups from 25 to 62 days post-infection, varying with the dose (IV1:4/6, IV2:3/6; IV4:2/5, IV3:1/6), and was three times less frequently associated with the SC route than IV inoculation (1/6 vs. 3/6). A dose-dependent effect for parasite loads in placental and foetal brain tissues was also detected. After SC challenge, a reduced number of tachyzoites were able to reach foetal brain tissues, and no lesions were observed. In calves, specific IgG responses in precolostral sera were mainly associated with high-dose groups (IV1 [100.0%] and IV2 [66.7%]), and cerebral parasite DNA detection was scarce (3/18). In dams, IFN-γ production and the dynamics of anti-N. caninum IgG antibodies varied with the dose, and the cell-mediated immune response was also found to be route-dependent. Our results confirm the influence of parasite dose and inoculation route on the outcome and dynamics of bovine neosporosis at mid-gestation. |
author2 |
Ministerio de Economía y Competitividad (España) |
author_facet |
Ministerio de Economía y Competitividad (España) Vázquez, Patricia Osoro, Koldo Fernández, Miguel Román-Trufero, Alicia Regidor-Cerrillo, Javier Jiménez-Pelayo, Laura García-Sánchez, Marta Rojo-Montejo, Silvia Benavides, Julio Horcajo, Pilar Ortega-Mora, Luis M. |
format |
artículo |
author |
Vázquez, Patricia Osoro, Koldo Fernández, Miguel Román-Trufero, Alicia Regidor-Cerrillo, Javier Jiménez-Pelayo, Laura García-Sánchez, Marta Rojo-Montejo, Silvia Benavides, Julio Horcajo, Pilar Ortega-Mora, Luis M. |
spellingShingle |
Vázquez, Patricia Osoro, Koldo Fernández, Miguel Román-Trufero, Alicia Regidor-Cerrillo, Javier Jiménez-Pelayo, Laura García-Sánchez, Marta Rojo-Montejo, Silvia Benavides, Julio Horcajo, Pilar Ortega-Mora, Luis M. Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation |
author_sort |
Vázquez, Patricia |
title |
Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation |
title_short |
Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation |
title_full |
Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation |
title_fullStr |
Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation |
title_full_unstemmed |
Effects of challenge dose and inoculation route of the virulent Neospora caninum Nc-Spain7 isolate in pregnant cattle at mid-gestation |
title_sort |
effects of challenge dose and inoculation route of the virulent neospora caninum nc-spain7 isolate in pregnant cattle at mid-gestation |
publisher |
BioMed Central |
publishDate |
2019-09-23 |
url |
http://hdl.handle.net/10261/191808 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100002911 http://dx.doi.org/10.13039/100007652 |
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