Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs
13 páginas, 3 figuras, 2 tablas.
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| Language: | English |
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Blackwell Publishing
2018
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| Subjects: | RNA-seq, Carcass traits, Gene expression, Sheep, |
| Online Access: | http://hdl.handle.net/10261/178796 http://dx.doi.org/10.13039/501100010198 |
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dig-igm-es-10261-1787962021-04-13T15:28:26Z Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs Suárez-Vega, Aroa Arranz, Juan José Pérez Pérez, Valentín de la Fuente, L. F. Mateo, J. Gutiérrez Gil, Beatriz Ministerio de Economía, Industria y Competitividad (España) Pérez Pérez, Valentín [0000-0003-0075-1587] RNA-seq Carcass traits Gene expression Sheep 13 páginas, 3 figuras, 2 tablas. Adipose deposits influence the quality of ruminant carcasses, and in suckling lambs, internal types of adipose deposits represent a notable proportion of total fat. The aim of this study was to perform a comparative analysis of the perirenal fat transcriptomes of suckling lambs from two breeds with different growth and carcass characteristics. The perirenal fat tissue from 14 suckling lambs (Assaf, n = 8; Churra, n = 6) was used for the RNA-seq analysis. The functional enrichment analysis of the 670 highly expressed genes (>150 fragments per kilobase of exon per million fragments mapped) in the perirenal fat transcriptome of both breeds revealed that the majority of these genes were involved in energy processes. The expression of the UCP1 gene, a classical biomarker of brown fat, and the presence of multilocular adipocytes in the two breeds supported the presence of brown fat at the transition stage towards white fat tissue. The differential expression analysis performed identified 373 differentially expressed genes (DEGs) between the two compared breeds. Brown/white fat gene biomarkers were not included in the list of DEGs. In Assaf lambs, DEGs were enriched in Gene Ontology (GO) biological processes related to fatty-acid oxidation, whereas in Churra lambs, the majority of the significantly enriched GO terms were related to cholesterol synthesis, which suggests that upregulated DEGs in Assaf lambs are implicated in fat burning, whereas the Churra upregulated DEGs are linked to fat accumulation. These results can help to increase knowledge of the genes controlling early fat deposition in ruminants and shed light on fundamental aspects of adipose tissue growth. This work was supported by the associated funding of aRamon y Cajal contract (RYC-2012-10230) from the Spanish Ministry of Economy, Industry and Competitiveness(MINECO). BGG is funded by the Ramon y Cajal Programme(RYC-2012-10230) from MINECO. We sincerely thank thestaff from the farm of the Faculty of Veterinary Sciences ofthe University of Leon for the management of lambs and the collaborating slaughterhouse Peer reviewed 2019-03-28T13:33:56Z 2019-03-28T13:33:56Z 2018 artículo http://purl.org/coar/resource_type/c_6501 Animal Genetics 49: 605- 617 (2018) 0268-9146 http://hdl.handle.net/10261/178796 10.1111/age.12725 http://dx.doi.org/10.13039/501100010198 en http://dx.doi.org/10.1111/age.12725 Sí open Blackwell Publishing |
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RNA-seq Carcass traits Gene expression Sheep RNA-seq Carcass traits Gene expression Sheep |
| spellingShingle |
RNA-seq Carcass traits Gene expression Sheep RNA-seq Carcass traits Gene expression Sheep Suárez-Vega, Aroa Arranz, Juan José Pérez Pérez, Valentín de la Fuente, L. F. Mateo, J. Gutiérrez Gil, Beatriz Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs |
| description |
13 páginas, 3 figuras, 2 tablas. |
| author2 |
Ministerio de Economía, Industria y Competitividad (España) |
| author_facet |
Ministerio de Economía, Industria y Competitividad (España) Suárez-Vega, Aroa Arranz, Juan José Pérez Pérez, Valentín de la Fuente, L. F. Mateo, J. Gutiérrez Gil, Beatriz |
| format |
artículo |
| topic_facet |
RNA-seq Carcass traits Gene expression Sheep |
| author |
Suárez-Vega, Aroa Arranz, Juan José Pérez Pérez, Valentín de la Fuente, L. F. Mateo, J. Gutiérrez Gil, Beatriz |
| author_sort |
Suárez-Vega, Aroa |
| title |
Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs |
| title_short |
Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs |
| title_full |
Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs |
| title_fullStr |
Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs |
| title_full_unstemmed |
Early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of Assaf and Churra suckling lambs |
| title_sort |
early adipose deposits in sheep: comparative analysis of the perirenal fat ranscriptome of assaf and churra suckling lambs |
| publisher |
Blackwell Publishing |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10261/178796 http://dx.doi.org/10.13039/501100010198 |
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