In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways
13 páginas, 4 figuras, 2 tablas.
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| Language: | English |
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American Dairy Science Association
2018
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| Subjects: | Cattle, Mass-spectrometry, PUFA, Ruminal lipid metabolism, Sheep, |
| Online Access: | http://hdl.handle.net/10261/166876 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 |
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dig-igm-es-10261-1668762022-06-23T10:05:56Z In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways Toral, Pablo G. Hervás, Gonzalo Leskinen, H. Shingfield, Kevin J. Frutos, Pilar Ministerio de Economía y Competitividad (España) European Commission Frutos, Pilar [0000-0002-4919-5094] Toral, Pablo G. [0000-0002-1913-7707] Hervás, Gonzalo [0000-0002-0013-7459] Cattle Mass-spectrometry PUFA Ruminal lipid metabolism Sheep 13 páginas, 4 figuras, 2 tablas. A great deal of uncertainty still exists about intermediate metabolites and pathways explaining the biohydrogenation (BH) of 20- and 22-carbon polyunsaturated fatty acids (PUFA). Therefore, this study was conducted to provide further insight into the ruminal metabolism of 20:5 n-3 (EPA), 22:5 n-3 (DPA), and 22:6 n-3 (DHA), the main n-3 PUFA present in the marine lipids used in dairy ruminant feeding, and to examine potential differences between bovine and ovine. To meet this aim, we investigated the 20- and 22-carbon metabolites accumulated during in vitro incubation of EPA, DPA, and DHA with rumen inocula from cows and ewes. The PUFA were added at a dose of 2% incubated dry matter and digesta samples were analyzed after 24 h of incubation using complementary gas-liquid chromatography of fatty acid methyl esters and gas chromatography-mass spectrometry of 4,4-dimethyloxazoline derivatives. Results suggested that the main BH pathway of EPA and DPA would proceed via the reduction of the double bond closest to the carboxyl group (cis-5 in EPA and cis-7 in DPA); curiously, this mechanism seemed of much lower importance for DHA. Thus, DPA would not be a major intermediate product of DHA and their BH might actually follow separate pathways, with the accumulation of numerous unique metabolites in each case. A principal component analysis supported this hypothesis, with a clear separation between PUFA treatments in the score and loading plots. Within EPA and DPA groups, cow and ewe samples loaded separately from each other but not distant. No conjugated 20:5, 22:5, or 22:6 isomer compatible with the initial product of EPA, DPA, or DHA metabolism, respectively, was identified in the ruminal digesta, although this would not unequivocally exclude their transient formation. In this regard, results from DPA incubations provided the first indication that the metabolism of this very long chain PUFA may involve the formation of conjugated double bond structures. The BH of EPA, DPA, and DHA resulted in the appearance of several tentative trans-10-containing metabolites, showing a general trend to be more abundant in the digesta of ewes than in that of cows. This finding was speculated to have some relationship with the susceptibility of dairy sheep to marine lipid-induced milk fat depression. Differences in the relative proportion of intermediate products would also suggest an influence of ruminant species on BH kinetics, with a process that would likely be slower and less complete in cows than in ewes. Copyright © 2018 American Dairy Science Association. Published by Elsevier Inc. All rights reserved. This work was supported by the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2014- 54587-R). P. G. Toral benefited from a Ramón y Cajal research contract from the MINECO. Co-funding by the European Regional Development Fund is also acknowledged. Peer reviewed 2018-06-22T10:27:37Z 2018-06-22T10:27:37Z 2018 artículo http://purl.org/coar/resource_type/c_6501 Journal of Dairy Science 101: 6109-6121 (2018) 1525-3198 http://hdl.handle.net/10261/166876 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-54587-R Postprint http://dx.doi.org/10.3168/jds.2017-14183 Sí open American Dairy Science Association |
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Cattle Mass-spectrometry PUFA Ruminal lipid metabolism Sheep Cattle Mass-spectrometry PUFA Ruminal lipid metabolism Sheep |
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Cattle Mass-spectrometry PUFA Ruminal lipid metabolism Sheep Cattle Mass-spectrometry PUFA Ruminal lipid metabolism Sheep Toral, Pablo G. Hervás, Gonzalo Leskinen, H. Shingfield, Kevin J. Frutos, Pilar In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways |
| description |
13 páginas, 4 figuras, 2 tablas. |
| author2 |
Ministerio de Economía y Competitividad (España) |
| author_facet |
Ministerio de Economía y Competitividad (España) Toral, Pablo G. Hervás, Gonzalo Leskinen, H. Shingfield, Kevin J. Frutos, Pilar |
| format |
artículo |
| topic_facet |
Cattle Mass-spectrometry PUFA Ruminal lipid metabolism Sheep |
| author |
Toral, Pablo G. Hervás, Gonzalo Leskinen, H. Shingfield, Kevin J. Frutos, Pilar |
| author_sort |
Toral, Pablo G. |
| title |
In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways |
| title_short |
In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways |
| title_full |
In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways |
| title_fullStr |
In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways |
| title_full_unstemmed |
In vitro ruminal biohydrogenation of eicosapentaenoic (EPA), docosapentaenoic (DPA), and docosahexaenoic acid (DHA) in cows and ewes: Intermediate metabolites and pathways |
| title_sort |
in vitro ruminal biohydrogenation of eicosapentaenoic (epa), docosapentaenoic (dpa), and docosahexaenoic acid (dha) in cows and ewes: intermediate metabolites and pathways |
| publisher |
American Dairy Science Association |
| publishDate |
2018 |
| url |
http://hdl.handle.net/10261/166876 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100003329 |
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