Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)

Mateos, Iván; Ranilla, María José; Saro, Cristina; Carro Travieso, Mª Dolores; Ministerio de Economía y Competitividad (España)

Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)

10 páginas, 4 tablas, 3 figuras.

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Bibliographic Details
Main Authors:	Mateos, Iván, Ranilla, María José, Saro, Cristina, Carro Travieso, Mª Dolores
Other Authors:	Ministerio de Economía y Competitividad (España)
Format:	artículo biblioteca
Published:	Cambridge University Press 2017
Subjects:	Microbial populations, qPCR, Sheep, Bacterial diversity, Rusitec fermenters,
Online Access:	http://hdl.handle.net/10261/162094 http://dx.doi.org/10.13039/501100003329
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spelling	dig-igm-es-10261-1620942021-03-18T16:55:29Z Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA) Mateos, Iván Ranilla, María José Saro, Cristina Carro Travieso, Mª Dolores Ministerio de Economía y Competitividad (España) Microbial populations qPCR Sheep Bacterial diversity Rusitec fermenters 10 páginas, 4 tablas, 3 figuras. Rusitec fermenters are in vitro systems widely used to study ruminal fermentation, but little is known about the microbial populations establishing in them. This study was designed to assess the time evolution of microbial populations in fermenters fed medium- (MC; 50% alfalfa hay : concentrate) and high-concentrate diets (HC; 15 : 85 barley straw : concentrate). Samples from solid (SOL) and liquid (LIQ) content of fermenters were taken immediately before feeding on days 3, 8 and 14 of incubation for quantitative polymerase chain reaction and automated ribosomal intergenic spacer analysis analyses. In SOL, total bacterial DNA concentration and relative abundance of Ruminococcus flavefaciens remained unchanged over the incubation period, but protozoal DNA concentration and abundance of Fibrobacter succinogenes, Ruminococcus albus and fungi decreased and abundance of methanogenic archaea increased. In LIQ, total bacterial DNA concentration increased with time, whereas concentration of protozoal DNA and abundance of methanogens and fungi decreased. Diet × time interactions were observed for bacterial and protozoal DNA and relative abundance of F. succinogenes and R. albus in SOL, as well as for protozoal DNA in LIQ. Bacterial diversity in SOL increased with time, but no changes were observed in LIQ. The incubated diet influenced all microbial populations, with the exception of total bacteria and fungi abundance in LIQ. Bacterial diversity was higher in MC-fed than in HC-fed fermenters in SOL, but no differences were detected in LIQ. Values of pH, daily production of volatile fatty acids and CH4 and isobutyrate proportions remained stable over the incubation period, but other fermentation parameters varied with time. The relationships among microbial populations and fermentation parameters were in well agreement with those previously reported in in vivo studies. Using 15N as a microbial marker or quantifying total microbial DNA for estimating microbial protein synthesis offered similar results for diets comparison, but both methods presented contrasting results for microbial growth in SOL and LIQ phases. The study showed that fermentation parameters remained fairly stable over the commonly used sampling period (days 8 to 14), but shifts in microbial populations were detected. Moreover, microbial populations differed markedly from those in the inocula, which indicates the difficulty of directly transposing results on microbial populations developed in Rusitec fermenters to in vivo conditions. This work was supported by the Spanish Ministry of Economy and Competitiveness under the Project AGL2011-22628. We would like to thank Dr Benjamin Rabanal for helping us with qPCR and ARISA analyses. Peer Reviewed 2018-03-13T09:07:54Z 2018-03-13T09:07:54Z 2017 2018-03-13T09:07:54Z artículo http://purl.org/coar/resource_type/c_6501 Animal 11: 1939- 1948 (2017) 1751-7311 http://hdl.handle.net/10261/162094 10.1017/S1751731117000878 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.1017/S1751731117000878 Sí none Cambridge University Press
institution	IGM ES
collection	DSpace
country	España
countrycode	ES
component	Bibliográfico
access	En linea
databasecode	dig-igm-es
tag	biblioteca
region	Europa del Sur
libraryname	Biblioteca del IGM España
topic	Microbial populations qPCR Sheep Bacterial diversity Rusitec fermenters Microbial populations qPCR Sheep Bacterial diversity Rusitec fermenters
spellingShingle	Microbial populations qPCR Sheep Bacterial diversity Rusitec fermenters Microbial populations qPCR Sheep Bacterial diversity Rusitec fermenters Mateos, Iván Ranilla, María José Saro, Cristina Carro Travieso, Mª Dolores Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)
description	10 páginas, 4 tablas, 3 figuras.
author2	Ministerio de Economía y Competitividad (España)
author_facet	Ministerio de Economía y Competitividad (España) Mateos, Iván Ranilla, María José Saro, Cristina Carro Travieso, Mª Dolores
format	artículo
topic_facet	Microbial populations qPCR Sheep Bacterial diversity Rusitec fermenters
author	Mateos, Iván Ranilla, María José Saro, Cristina Carro Travieso, Mª Dolores
author_sort	Mateos, Iván
title	Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)
title_short	Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)
title_full	Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)
title_fullStr	Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)
title_full_unstemmed	Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)
title_sort	shifts in microbial populations in rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15n v. microbial dna)
publisher	Cambridge University Press
publishDate	2017
url	http://hdl.handle.net/10261/162094 http://dx.doi.org/10.13039/501100003329
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_version_	1777664020817379328

Shifts in microbial populations in Rusitec fermenters as affected by the type of diet and impact of the method for estimating microbial growth (15N v. microbial DNA)

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