Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period
19 páginas, 8 tablas, 3 figuras.
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American Dairy Science Association
2017
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| Subjects: | Rumen epithelium, Rumen adaptation, VFA absorption and metabolism, Gene and protein expression, |
| Online Access: | http://hdl.handle.net/10261/158534 |
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dig-igm-es-10261-1585342018-01-02T10:34:55Z Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period Dieho, K. van Baal, J. Kruijt, L. Bannink, A. Schonewille, J. T. Carreño, David Hendriks, W.H. Dijkstra, J. Dutch Animal Feed Product Board Dutch Dairy Board Rumen epithelium Rumen adaptation VFA absorption and metabolism Gene and protein expression 19 páginas, 8 tablas, 3 figuras. We previously reported 2 experiments with rumen-cannulated Holstein-Friesian dairy cows showing that during the transition period, rumen papillae surface area, and fractional absorption rate of volatile fatty acids (VFA) increase after calving. However, supplemental concentrate during the dry period and rate of increase of concentrate allowance during lactation affected papillae surface area, but not VFA absorption. Here we report the changes in gene and protein expression in rumen papillae related to tissue growth and VFA utilization. The lactation experiment treatment consisted of a rapid [RAP; 1.0 kg of dry matter (DM)/d; n = 6] or gradual (GRAD; 0.25 kg of DM/d; n = 6) increase of concentrate allowance (up to 10.9 kg of DM/d), starting at 4 d postpartum (pp). The dry period experiment treatment consisted of 3.0 kg of DM/d of concentrate (n = 4) or no concentrate (n = 5) during the last 28 d of the dry period. Real-time quantitative PCR analysis of rumen papillae showed that the expression of apoptosis-related genes was neither affected by day nor its interaction with treatment for both experiments. Expression of epithelial transporter genes was not affected by day or treatment in the lactation experiment, except for NBC1. In the dry period experiment, expression of MCT1, NBC1, DRA, NHE2, NHE3, and UT-B generally decreased after calving. A day and treatment interaction was observed for ATP1A1 in the dry period experiment, with greater expression at 18 and 8 d antepartum for concentrate than no concentrate. Generally, expression of VFA metabolism-related genes was not affected by day or its interaction with treatment. In the lactation experiment, immunoblotting of 5 selected genes showed that protein expression of DRA and PCCA was greater at 16 d pp compared with 3 and 44 d pp. Expression of NHE2 was greater, and that of ATP1A1 lower, at 16 and 44 d pp compared with 3 d pp, suggesting alterations in intracellular pH regulation and sodium homeostasis. Both MCT1 and PCCA protein were upregulated by RAP from 3 to 16 d pp, indicating modulations in VFA metabolism. Our data suggests that VFA absorption and metabolic capacity changed little per unit of surface area during the transition period, and suggests that a change in mitosis rate rather than apoptosis rate is associated with the increased ruminal VFA production, resulting in tissue growth. A significant but weak correlation between the examined gene and protein expression levels was observed only for PCCA, indicating that care must be taken when interpreting results obtained at either level. The authors acknowledge the Dairy Campus staff (Lelystad, the Netherlands) for their technical support during the experiments. The authors acknowledge the financial support of the Product Board Animal Feed (Zoetermeer, the Netherlands) and Dutch Dairy Board (Zoetermeer, the Netherlands). Peer Reviewed 2017-12-22T12:51:26Z 2017-12-22T12:51:26Z 2017 2017-12-22T12:51:27Z artículo http://purl.org/coar/resource_type/c_6501 Journal of Dairy Science 100: 7227- 7245 (2017) 1525-3198 http://hdl.handle.net/10261/158534 10.3168/jds.2016-12403 http://dx.doi.org/10.3168/jds.2016-12403 Sí none American Dairy Science Association |
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Rumen epithelium Rumen adaptation VFA absorption and metabolism Gene and protein expression Rumen epithelium Rumen adaptation VFA absorption and metabolism Gene and protein expression |
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Rumen epithelium Rumen adaptation VFA absorption and metabolism Gene and protein expression Rumen epithelium Rumen adaptation VFA absorption and metabolism Gene and protein expression Dieho, K. van Baal, J. Kruijt, L. Bannink, A. Schonewille, J. T. Carreño, David Hendriks, W.H. Dijkstra, J. Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period |
| description |
19 páginas, 8 tablas, 3 figuras. |
| author2 |
Dutch Animal Feed Product Board |
| author_facet |
Dutch Animal Feed Product Board Dieho, K. van Baal, J. Kruijt, L. Bannink, A. Schonewille, J. T. Carreño, David Hendriks, W.H. Dijkstra, J. |
| format |
artículo |
| topic_facet |
Rumen epithelium Rumen adaptation VFA absorption and metabolism Gene and protein expression |
| author |
Dieho, K. van Baal, J. Kruijt, L. Bannink, A. Schonewille, J. T. Carreño, David Hendriks, W.H. Dijkstra, J. |
| author_sort |
Dieho, K. |
| title |
Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period |
| title_short |
Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period |
| title_full |
Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period |
| title_fullStr |
Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period |
| title_full_unstemmed |
Effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period |
| title_sort |
effect of supplemental concentrate during the dry period or early lactation on rumen epithelium gene and protein expression in dairy cattle during the transition period |
| publisher |
American Dairy Science Association |
| publishDate |
2017 |
| url |
http://hdl.handle.net/10261/158534 |
| work_keys_str_mv |
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