Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes
11 páginas, 5 tablas.
Saved in:
| Main Authors: | , , , , |
|---|---|
| Other Authors: | |
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
American Dairy Science Association
2016-10
|
| Subjects: | Fatty acid, Gene expression, Marine lipid, Nutrigenomics, Sheep, |
| Online Access: | http://hdl.handle.net/10261/137066 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100014180 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-igm-es-10261-137066 |
|---|---|
| record_format |
koha |
| institution |
IGM ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-igm-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del IGM España |
| language |
English |
| topic |
Fatty acid Gene expression Marine lipid Nutrigenomics Sheep Fatty acid Gene expression Marine lipid Nutrigenomics Sheep |
| spellingShingle |
Fatty acid Gene expression Marine lipid Nutrigenomics Sheep Fatty acid Gene expression Marine lipid Nutrigenomics Sheep Carreño, David Hervás, Gonzalo Toral, Pablo G. Castro Carrera, Tamara Frutos, Pilar Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes |
| description |
11 páginas, 5 tablas. |
| author2 |
Junta de Castilla y León |
| author_facet |
Junta de Castilla y León Carreño, David Hervás, Gonzalo Toral, Pablo G. Castro Carrera, Tamara Frutos, Pilar |
| format |
artículo |
| topic_facet |
Fatty acid Gene expression Marine lipid Nutrigenomics Sheep |
| author |
Carreño, David Hervás, Gonzalo Toral, Pablo G. Castro Carrera, Tamara Frutos, Pilar |
| author_sort |
Carreño, David |
| title |
Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes |
| title_short |
Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes |
| title_full |
Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes |
| title_fullStr |
Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes |
| title_full_unstemmed |
Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes |
| title_sort |
fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes |
| publisher |
American Dairy Science Association |
| publishDate |
2016-10 |
| url |
http://hdl.handle.net/10261/137066 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100014180 |
| work_keys_str_mv |
AT carrenodavid fishoilinducedmilkfatdepressionandassociateddownregulationofmammarylipogenicgenesindairyewes AT hervasgonzalo fishoilinducedmilkfatdepressionandassociateddownregulationofmammarylipogenicgenesindairyewes AT toralpablog fishoilinducedmilkfatdepressionandassociateddownregulationofmammarylipogenicgenesindairyewes AT castrocarreratamara fishoilinducedmilkfatdepressionandassociateddownregulationofmammarylipogenicgenesindairyewes AT frutospilar fishoilinducedmilkfatdepressionandassociateddownregulationofmammarylipogenicgenesindairyewes |
| _version_ |
1777664000558891008 |
| spelling |
dig-igm-es-10261-1370662022-06-23T09:42:31Z Fish oil-induced milk fat depression and associated downregulation of mammary lipogenic genes in dairy ewes Carreño, David Hervás, Gonzalo Toral, Pablo G. Castro Carrera, Tamara Frutos, Pilar Junta de Castilla y León Ministerio de Economía y Competitividad (España) European Commission Fatty acid Gene expression Marine lipid Nutrigenomics Sheep 11 páginas, 5 tablas. Several studies in dairy cows have shown a relationship between milk fat depression (MFD) and alterations caused in lipogenic gene expression by dietary nutrients. However, information on small ruminants is not only scarce but also inconsistent. Therefore, this experiment was conducted in dairy ewes to study the effect of a diet known to induce MFD on milk fatty acid (FA) composition and mRNA abundance of key candidate genes involved in mammary lipogenesis. Twelve lactating Assaf ewes (on average 63d in milk) were randomly assigned to 2 treatments consisting of a total mixed ration based on alfalfa hay and concentrates (50:50), supplemented with 0 (control) or 17g of fish oil/kg of diet dry matter (FO). Profiles of milk FA and mRNA abundance of candidate genes in biopsied mammary tissue were examined before starting the treatments and after 1 and 4.5wk on the diets. As expected, FO induced MFD and modified milk FA composition. Compared with the control, reductions in milk fat concentration and yield were not detected on d 7, but reached up to 25 and 22%, respectively, on d 30. However, increases in confirmed or putative antilipogenic FA (trans-10,cis-12 and trans-9,cis-11 18:2, cis-9 16:1, cis-11 18:1, and oxo-FA) were already established on the early stage of the treatment and lasted until the end of the feeding period. These changes were accompanied by decreases in the mRNA abundance of genes encoding lipogenic enzymes. The coordinated nature of the downregulation, which tended to affect most studied metabolic pathways, including FA activation (ACSS1), de novo synthesis (ACACA and FASN), uptake and transport (LPL and FABP3), desaturation (SCD1), and esterification (AGAPT6), supports the involvement of a central regulator of milk fat synthesis. In this regard, without ruling out the potential contribution of PPARG, our results suggest that SREBF1 would have a relevant role in the MFD syndrome in sheep fed FO. Among the other studied transcription factors, the tendency to a downregulation of INSIG1 was associated with that of SREBF1, whereas no variation was detected for SCAP or THRSP. Fish oil had no significant effects on the transcript abundance of CD36, GPAM, DGAT1, LPIN1, and XDH. Overall, changes in potential antilipogenic FA and mRNA abundance of candidate lipogenic genes support a relationship between them and suggest that FO-induced MFD in dairy ewes would be mediated by transcriptional mechanisms. This work was supported by the Council of Castile and Leon (CSI023U13) and the Spanish Ministry of Economy and Competitiveness (MINECO; AGL2014-54587, co-funded by the European Regional Development Fund). D. Carreño and P. G. Toral benefitted from an FPI predoctoral grant and a Juan de la Cierva research contract, respectively, from the MINECO. The authors thank A. González-Mendoza from the Instituto de Ganadería de Montaña (Spain) for his help in the field work and laboratory analysis. Special gratitude is extended to Y. Chilliard, L. Bernard, C. Leroux (INRA, Clermont-Ferrand, France), and J. J. Arranz (University of León, Spain), and also to L. Barrios (Biostatistics Department, CSIC; Madrid, Spain) for helpful assistance with nutrigenomic techniques and statistical analysis, respectively. Peer reviewed 2016-09-20T12:31:15Z 2016-09-20T12:31:15Z 2016-10 artículo http://purl.org/coar/resource_type/c_6501 Journal of Dairy Science 99 (10): 7971-7981 (2016) 0022-0302 http://hdl.handle.net/10261/137066 10.3168/jds.2016-11019 http://dx.doi.org/10.13039/501100003329 http://dx.doi.org/10.13039/501100000780 http://dx.doi.org/10.13039/501100014180 en #PLACEHOLDER_PARENT_METADATA_VALUE# info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2014-54587-R Postprint http://dx.doi.org/10.3168/jds.2016-11019 Sí open American Dairy Science Association |