Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms

Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms

Trabajo presentado al: Joint Annual Meeting. ADSA-ASAS.( Orlando, Florida, USA, 12-16 julio 2015. T486

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Main Authors: Ramos, M., Mateos, Iván, Saro, Cristina, Díaz, A., Ranilla, María José
Format: póster de congreso biblioteca
Language:English
Published: American Society of Animal Science 2015
Subjects:Rumen content treatment, Methane, Volatile fatty acids,
Online Access:http://hdl.handle.net/10261/130460
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spelling dig-igm-es-10261-1304602021-03-18T16:55:29Z Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms Ramos, M. Mateos, Iván Saro, Cristina Díaz, A. Ranilla, María José Rumen content treatment Methane Volatile fatty acids Trabajo presentado al: Joint Annual Meeting. ADSA-ASAS.( Orlando, Florida, USA, 12-16 julio 2015. T486 The characteristics of the inoculum are recognized as one of the most relevant factors infiuencing the results o fin vitro fennentations in batch cultures of ruminal microorganisms. Four rumen-fistulated sheep fed a 66:34 alfalfa hay:concentrate diet were used as donors to investigate the effect ofrumen contents' processing on in vitro methane (CH4) and volatile fatty acid (VFA) production from 3 substrates ofvariable composition. Rumen contents were sampled from each individual sheep and subjected to the following treatments: SQ) squeezed through 4 layers of cheesecloth; FIL) SQ treatment and further filtration through a 100-µm nylon cloth; STO) treated with a Stomacher for 3 min at 230 rev min-1 and followed by SQ. The resulting fiuids were used as inoculum for batch cultures containing alfalfa hay, concentrate, or a 50:50 mixtnre ofboth feeds. Cultures were incubated at 39°C for 8 and 24 h, and CH4 and VF A production was measured. There were no treatment x substrate interactions (P > 0.05) for any variable at any incubation time, excepting for the molar proportion of acetate at 24 h (P = 0.019). The method of processing the rumen contents did not affect (P > 0.05) eithertotal VFA and CH4 production or molar proportions of individual VF A at any time. At both incubation times, increasing the amount of concentrate in the substrate increased CH4 production (P < 0.001, quadratic) and molar proportion ofbutyrate (P < 0.001, linear), but decreased acetate proportion (P < 0.001, quadratic) without affecting (P > 0.05) proportions of propionate. Whereas total VFA production was linearly decreased (P = 0.007) by increased amounts of concentrate in the substrate at 8 h ofincubation, it was quadratically increased (P < 0.001) after 24 h of incubation. There were clear differences in CH4 and VFA production among inocula from different sheep, which persisted across substrates. The results show that the tested methods ofprocessing rumen contents did not affect in vitro fermentation characteristics of good quality substrates, but studies ana!yzing their possible infiuence on fermentation of!ow-quality substrates are required Peer reviewed 2016-03-28T12:36:18Z 2016-03-28T12:36:18Z 2015 póster de congreso http://purl.org/coar/resource_type/c_6670 Journal of Animal Science 93 (Suppl. s3/J) 484 (2015) http://hdl.handle.net/10261/130460 1525-3163 en http://www.jtmtg.org/jam/2015/ Sí none American Society of Animal Science
institution IGM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-igm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IGM España
language English
topic Rumen content treatment
Methane
Volatile fatty acids
Rumen content treatment
Methane
Volatile fatty acids
spellingShingle Rumen content treatment
Methane
Volatile fatty acids
Rumen content treatment
Methane
Volatile fatty acids
Ramos, M.
Mateos, Iván
Saro, Cristina
Díaz, A.
Ranilla, María José
Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
description Trabajo presentado al: Joint Annual Meeting. ADSA-ASAS.( Orlando, Florida, USA, 12-16 julio 2015. T486
format póster de congreso
topic_facet Rumen content treatment
Methane
Volatile fatty acids
author Ramos, M.
Mateos, Iván
Saro, Cristina
Díaz, A.
Ranilla, María José
author_facet Ramos, M.
Mateos, Iván
Saro, Cristina
Díaz, A.
Ranilla, María José
author_sort Ramos, M.
title Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
title_short Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
title_full Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
title_fullStr Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
title_full_unstemmed Influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
title_sort influence of inoculum preparation method on in vitro methane production by ruminal microorganisms
publisher American Society of Animal Science
publishDate 2015
url http://hdl.handle.net/10261/130460
work_keys_str_mv AT ramosm influenceofinoculumpreparationmethodoninvitromethaneproductionbyruminalmicroorganisms
AT mateosivan influenceofinoculumpreparationmethodoninvitromethaneproductionbyruminalmicroorganisms
AT sarocristina influenceofinoculumpreparationmethodoninvitromethaneproductionbyruminalmicroorganisms
AT diaza influenceofinoculumpreparationmethodoninvitromethaneproductionbyruminalmicroorganisms
AT ranillamariajose influenceofinoculumpreparationmethodoninvitromethaneproductionbyruminalmicroorganisms
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