Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)

Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)

8 pages, 4 tables, 3 figures.--PMID: 15333153 [PubMed].

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Main Authors: Guerouali, Abdelhai, El Gass, Youssef, Balcells, Joaquín, Belenguer, Álvaro, Nolan, John
Format: artículo biblioteca
Language:English
Published: Cambridge University Press 2004
Subjects:Purine derivatives, Xanthine oxidase, Microbial protein, Camels,
Online Access:http://hdl.handle.net/10261/12129
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spelling dig-igm-es-10261-121292018-08-27T08:31:43Z Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius) Guerouali, Abdelhai El Gass, Youssef Balcells, Joaquín Belenguer, Álvaro Nolan, John Purine derivatives Xanthine oxidase Microbial protein Camels 8 pages, 4 tables, 3 figures.--PMID: 15333153 [PubMed]. Five experiments were carried out to extend knowledge of purine metabolism in the camel (Camelus dromedarius) and to establish a model to enable microbial protein outflow from the forestomachs to be estimated from the urinary excretion of purine derivatives (PD; i.e. xanthine, hypoxanthine, uric acid, allantoin). In experiment 1, four camels were fasted for five consecutive days to enable endogenous PD excretion in urine to be determined. Total PD excretion decreased during the fasting period to 267 (SE 41.5) micromol/kg body weight (W)0.75 per d. Allantoin and xanthine + hypoxanthine were consistently 86 and 6.1 % of total urinary PD during this period but uric acid increased from 3.6 % to 7.4 %. Xanthine oxidase activity in tissues (experiment 2) was (micromol/min per g fresh tissue) 0.038 in liver and 0.005 in gut mucosa but was not detected in plasma. In experiment 3, the duodenal supply of yeast containing exogenous purines produced a linear increase in urinary PD excretion rate with the slope indicating that 0.63 was excreted in urine. After taking account of endogenous PD excretion, the relationship can be used to predict purine outflow from the rumen. From the latter prediction, and also the purine:protein ratio in bacteria determined in experiment 5, we predicted the net microbial outflow from the rumen. In experiment 4, with increasing food intake, the rate of PD excretion in the urine increased linearly by about 11.1 mmol PD/kg digestible organic matter intake (DOMI), equivalent to 95 g microbial protein/kg DOMI. The present study was supported by ACSAD and the IAEA via research contract MOR10564 (Morocco) and AA.EE/AECI 229-188 and CICYT- PB 98-1601 (Spain). Peer reviewed 2009-04-06T08:58:49Z 2009-04-06T08:58:49Z 2004 artículo http://purl.org/coar/resource_type/c_6501 British Journal of Nutrition 92(2): 225-232 (2004) 0007-1145 http://hdl.handle.net/10261/12129 10.1079/BJN20041208 en http://dx.doi.org/10.1079/BJN20041208 open 148845 bytes application/pdf Cambridge University Press
institution IGM ES
collection DSpace
country España
countrycode ES
component Bibliográfico
access En linea
databasecode dig-igm-es
tag biblioteca
region Europa del Sur
libraryname Biblioteca del IGM España
language English
topic Purine derivatives
Xanthine oxidase
Microbial protein
Camels
Purine derivatives
Xanthine oxidase
Microbial protein
Camels
spellingShingle Purine derivatives
Xanthine oxidase
Microbial protein
Camels
Purine derivatives
Xanthine oxidase
Microbial protein
Camels
Guerouali, Abdelhai
El Gass, Youssef
Balcells, Joaquín
Belenguer, Álvaro
Nolan, John
Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)
description 8 pages, 4 tables, 3 figures.--PMID: 15333153 [PubMed].
format artículo
topic_facet Purine derivatives
Xanthine oxidase
Microbial protein
Camels
author Guerouali, Abdelhai
El Gass, Youssef
Balcells, Joaquín
Belenguer, Álvaro
Nolan, John
author_facet Guerouali, Abdelhai
El Gass, Youssef
Balcells, Joaquín
Belenguer, Álvaro
Nolan, John
author_sort Guerouali, Abdelhai
title Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)
title_short Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)
title_full Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)
title_fullStr Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)
title_full_unstemmed Urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (Camelus dromedarius)
title_sort urinary excretion of purine derivatives as an index of microbial protein synthesis in the camel (camelus dromedarius)
publisher Cambridge University Press
publishDate 2004
url http://hdl.handle.net/10261/12129
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