Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques
12 pages, 5 tables.-- Available online Jun 6, 2005.
Saved in:
| Main Authors: | , , , , , |
|---|---|
| Format: | artículo biblioteca |
| Language: | English |
| Published: |
Elsevier
2005
|
| Subjects: | In vitro gas production technique, Rumen inoculum, Storage, Temperatures, Ruminal fermentation, |
| Online Access: | http://hdl.handle.net/10261/10181 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
dig-igm-es-10261-10181 |
|---|---|
| record_format |
koha |
| spelling |
dig-igm-es-10261-101812018-09-04T11:11:53Z Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques Hervás, Gonzalo Frutos, Pilar Giráldez, Francisco Javier Mora Martínez, Manuel José Fernández, Begoña Mantecón, Ángel R. In vitro gas production technique Rumen inoculum Storage Temperatures Ruminal fermentation 12 pages, 5 tables.-- Available online Jun 6, 2005. Use of preserved rumen fluid (RF) as microbial inoculum for in vitro gas production techniques (IVGPT) would be of practical importance when access to ruminally cannulated animals is limited. This experiment was designed to study effects of RF preservation on its subsequent fermentative activity. Four substrates being: alfalfa hay (AH), barley straw NDF (BS-NDF), cellulose and starch were incubated with rumen inoculum obtained from 12 rumen cannulated grazing sheep, of which six (G) received no supplement and the other 6 (G+S) received 500 g/day of a concentrate. Sub-samples of RF were processed without delay (i.e., control) or stored at approximately 0°C in a chest with crushed ice for 3, 6 and 24 h (treatments 3, 6 and 24 h, respectively) or at −18 °C for 24 h in a freezer (treatment 24 F). An IVGPT was used to determine the rate and extent of gas production and DM disappearance. Average fermentation rate (AFR) and extent of degradation in the rumen (ED) were estimated. Incubations with inoculum derived from supplemented sheep (G+S) resulted in faster rates of gas production (with differences, as proportionate increases, of 0.77 for the barley straw NDF; P < 0.01), lower lag times (e.g., 13.2 versus 4.0 h for the starch; P < 0.05), and higher ED values (e.g., 229 versus 331 g/kg for the barley straw NDF or 173 versus 409 g/kg for the cellulose; P < 0.05). Storing RF in ice for 3 or 6 h had no effect on any fermentation parameter. Treatment 24 h did not affect IVGPT parameters when starch and cellulose were fermented, but estimates of rates and ED were lower (P < 0.05) with BS-NDF and AH. Fermentative activity of rumen inoculum was, in general, reduced by freezing (24 F). However, incubations of starch and cellulose were less affected by frozen inoculum than those of AH or BS-NDF. Preservation of RF for up to 6 h in crushed ice may be a practical alternative, when necessary, to use fresh RF as microbial inoculum. G. Hervás and M.J. Mora gratefully acknowledge receipt of a research contract and a predoctoral grant, respectively, from the Spanish Council for Scientific Research (CSIC, Spain) supported by the European Social Fund. Peer reviewed 2009-02-02T12:39:20Z 2009-02-02T12:39:20Z 2005 artículo http://purl.org/coar/resource_type/c_6501 Animal Feed Science and Technology 123-124(1): 107-118 (2005) 0377-8401 http://hdl.handle.net/10261/10181 10.1016/j.anifeedsci.2005.05.004 en http://dx.doi.org/10.1016/j.anifeedsci.2005.05.004 open 94510 bytes application/pdf Elsevier |
| institution |
IGM ES |
| collection |
DSpace |
| country |
España |
| countrycode |
ES |
| component |
Bibliográfico |
| access |
En linea |
| databasecode |
dig-igm-es |
| tag |
biblioteca |
| region |
Europa del Sur |
| libraryname |
Biblioteca del IGM España |
| language |
English |
| topic |
In vitro gas production technique Rumen inoculum Storage Temperatures Ruminal fermentation In vitro gas production technique Rumen inoculum Storage Temperatures Ruminal fermentation |
| spellingShingle |
In vitro gas production technique Rumen inoculum Storage Temperatures Ruminal fermentation In vitro gas production technique Rumen inoculum Storage Temperatures Ruminal fermentation Hervás, Gonzalo Frutos, Pilar Giráldez, Francisco Javier Mora Martínez, Manuel José Fernández, Begoña Mantecón, Ángel R. Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques |
| description |
12 pages, 5 tables.-- Available online Jun 6, 2005. |
| format |
artículo |
| topic_facet |
In vitro gas production technique Rumen inoculum Storage Temperatures Ruminal fermentation |
| author |
Hervás, Gonzalo Frutos, Pilar Giráldez, Francisco Javier Mora Martínez, Manuel José Fernández, Begoña Mantecón, Ángel R. |
| author_facet |
Hervás, Gonzalo Frutos, Pilar Giráldez, Francisco Javier Mora Martínez, Manuel José Fernández, Begoña Mantecón, Ángel R. |
| author_sort |
Hervás, Gonzalo |
| title |
Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques |
| title_short |
Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques |
| title_full |
Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques |
| title_fullStr |
Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques |
| title_full_unstemmed |
Effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques |
| title_sort |
effect of preservation on fermentative activity of rumen fluid inoculum for in vitro gas production techniques |
| publisher |
Elsevier |
| publishDate |
2005 |
| url |
http://hdl.handle.net/10261/10181 |
| work_keys_str_mv |
AT hervasgonzalo effectofpreservationonfermentativeactivityofrumenfluidinoculumforinvitrogasproductiontechniques AT frutospilar effectofpreservationonfermentativeactivityofrumenfluidinoculumforinvitrogasproductiontechniques AT giraldezfranciscojavier effectofpreservationonfermentativeactivityofrumenfluidinoculumforinvitrogasproductiontechniques AT moramartinezmanueljose effectofpreservationonfermentativeactivityofrumenfluidinoculumforinvitrogasproductiontechniques AT fernandezbegona effectofpreservationonfermentativeactivityofrumenfluidinoculumforinvitrogasproductiontechniques AT manteconangelr effectofpreservationonfermentativeactivityofrumenfluidinoculumforinvitrogasproductiontechniques |
| _version_ |
1777663782715129856 |