Topoisomerase II is required for the production of long Pol II gene transcripts in yeast
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Oxford University Press
2012
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dig-idaea-es-10261-881062021-12-27T15:55:08Z Topoisomerase II is required for the production of long Pol II gene transcripts in yeast Joshi, Ricky S. Piña, Benjamín Roca, Joaquim This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License. The extent to which the DNA relaxation activities of eukaryotic topoisomerases (topo I and topo II) are redundant during gene transcription is unclear. Although both enzymes can often substitute for each other in vivo, studies in vitro had revealed that the DNA cross-inversion mechanism of topo II relaxes chromatin more efficiently than the DNA strand-rotation mechanism of topo I. Here, we show that the inactivation of topo II in budding yeast produces an abrupt decrease of virtually all polyA+ RNA transcripts of length above ~3¿kb, irrespective of their function. This reduction is not related to transcription initiation but to the stall of RNA polymerase II (Pol II) during elongation. This reduction does not occur in topo I mutants; and it is not avoided by overproducing yeast topo I or bacterial topo I, which relaxes (-) DNA supercoils. It is rescued by catalytically active topo II or a GyrBA enzyme, which relaxes (+) DNA supercoils. These findings demonstrate that DNA relaxation activities of topo I and topo II are not interchangeable in vivo. Apparently, only topo II relaxes efficiently the (+) DNA supercoils that stall the advancement of Pol II in long genes. A mechanistic model is proposed. Plan Nacional I + D + I of Spain [AGL2000-0133 and BIO2005-00840 to B.P.; BFU2008-00366 and BFU2011-23851 to J.R.]. Funding for open access charge: Plan Nacional I+D+I of Spain [BFU2011-23851 to J.R.]. Peer Reviewed 2013-12-03T12:22:25Z 2013-12-03T12:22:25Z 2012 2013-12-03T12:22:25Z artículo http://purl.org/coar/resource_type/c_6501 doi: 10.1093/nar/gks626 issn: 0305-1048 e-issn: 1362-4962 Nucleic Acids Research 40(16): 7907-7915 (2012) http://hdl.handle.net/10261/88106 10.1093/nar/gks626 22718977 en http://dx.doi.org/10.1093/nar/gks626 open Oxford University Press |
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España |
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ES |
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dig-idaea-es |
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biblioteca |
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Biblioteca del IDAEA España |
| language |
English |
| description |
This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License. |
| format |
artículo |
| author |
Joshi, Ricky S. Piña, Benjamín Roca, Joaquim |
| spellingShingle |
Joshi, Ricky S. Piña, Benjamín Roca, Joaquim Topoisomerase II is required for the production of long Pol II gene transcripts in yeast |
| author_facet |
Joshi, Ricky S. Piña, Benjamín Roca, Joaquim |
| author_sort |
Joshi, Ricky S. |
| title |
Topoisomerase II is required for the production of long Pol II gene transcripts in yeast |
| title_short |
Topoisomerase II is required for the production of long Pol II gene transcripts in yeast |
| title_full |
Topoisomerase II is required for the production of long Pol II gene transcripts in yeast |
| title_fullStr |
Topoisomerase II is required for the production of long Pol II gene transcripts in yeast |
| title_full_unstemmed |
Topoisomerase II is required for the production of long Pol II gene transcripts in yeast |
| title_sort |
topoisomerase ii is required for the production of long pol ii gene transcripts in yeast |
| publisher |
Oxford University Press |
| publishDate |
2012 |
| url |
http://hdl.handle.net/10261/88106 |
| work_keys_str_mv |
AT joshirickys topoisomeraseiiisrequiredfortheproductionoflongpoliigenetranscriptsinyeast AT pinabenjamin topoisomeraseiiisrequiredfortheproductionoflongpoliigenetranscriptsinyeast AT rocajoaquim topoisomeraseiiisrequiredfortheproductionoflongpoliigenetranscriptsinyeast |
| _version_ |
1777669248385024000 |