Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues
The analysis of pesticides residues using a last generation high resolution and high mass accuracy hybrid linear ion trap-Orbitrap mass spectrometer (LTQ-Orbitrap-MS) was explored. Pesticides were extracted from fruits, fish, bees and sediments by QuEChERS and from water by solid-phase with Oasis HLB cartridges. Ultra-high pressure liquid chromatography (UHPLC)-LTQ-Orbitrap mass spectrometer acquired full scan MS data for quantification, and data dependent (dd) MS(2) and MS(3) product ion spectra for identification and/or confirmation. The regression coefficients (r(2)) for the calibration curves (two order of magnitude up to the lowest calibration level) in the study were ≥0.99. The LODs for 54 validated compounds were ≤2ngmL(-1) (analytical standards). The relative standard deviation (RSD), which was used to estimate precision, was always lower than 22%. The recovery of extraction and matrix effects ranged from 58 to 120% and from -92 to 52%, respectively. Mass accuracy was always ≤4ppm, corresponding to a maximum mass error of 1.6millimass units (mmu). This procedure was then successfully applied to pesticide residues in a set of the above-mentioned food and environmental samples. In addition to target analytes, this method enables the simultaneous detection/identification of non-target pesticides, pharmaceuticals, drugs of abuse, mycotoxins, and their metabolites.
Main Authors: | Farré, Marinella, Picó, Yolanda, Barceló, Damià |
---|---|
Format: | artículo biblioteca |
Language: | English |
Published: |
Elsevier
2014-02-07
|
Subjects: | UHPLC, Contaminants identification, Environmental samples, Food samples, LTQ-Orbitrap-MS, Pesticide quantification, Ensure availability and sustainable management of water and sanitation for all, |
Online Access: | http://hdl.handle.net/10261/346069 https://api.elsevier.com/content/abstract/scopus_id/84892958135 |
Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
Similar Items
-
Enhancing Molecular Characterization of Dissolved Organic Matter by Integrative Direct Infusion and Liquid Chromatography Nontargeted Workflows
by: Patrone, Jessica, et al.
Published: (2024-07-16) -
Summary recommendations on "Analytical methods for substances in the Watch List under the Water Framework Directive"
by: Loos, Robert, et al.
Published: (2023-11-20) -
HRMS-based suspect screening of pharmaceuticals and their transformation products in multiple environmental compartments: An alternative to target analysis?
by: Castaño-Ortiz, Jose M, et al.
Published: (2023-12-29) -
Extended Suspect and Non-Target Strategies to Characterize Emerging Polar Organic Contaminants in Raw Wastewater with LC-HRMS/MS
by: Gago-Ferrero, Pablo, et al.
Published: (2015-10-20) -
Determination of Perfluorooctanoic Acid (PFOA) in the Indoor Dust Matter of the Sicily (Italy) Area: Analysis and Exposure Evaluations
by: Barreca, Salvatore, et al.
Published: (2024)