Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues
The analysis of pesticides residues using a last generation high resolution and high mass accuracy hybrid linear ion trap-Orbitrap mass spectrometer (LTQ-Orbitrap-MS) was explored. Pesticides were extracted from fruits, fish, bees and sediments by QuEChERS and from water by solid-phase with Oasis HLB cartridges. Ultra-high pressure liquid chromatography (UHPLC)-LTQ-Orbitrap mass spectrometer acquired full scan MS data for quantification, and data dependent (dd) MS(2) and MS(3) product ion spectra for identification and/or confirmation. The regression coefficients (r(2)) for the calibration curves (two order of magnitude up to the lowest calibration level) in the study were ≥0.99. The LODs for 54 validated compounds were ≤2ngmL(-1) (analytical standards). The relative standard deviation (RSD), which was used to estimate precision, was always lower than 22%. The recovery of extraction and matrix effects ranged from 58 to 120% and from -92 to 52%, respectively. Mass accuracy was always ≤4ppm, corresponding to a maximum mass error of 1.6millimass units (mmu). This procedure was then successfully applied to pesticide residues in a set of the above-mentioned food and environmental samples. In addition to target analytes, this method enables the simultaneous detection/identification of non-target pesticides, pharmaceuticals, drugs of abuse, mycotoxins, and their metabolites.
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Language: | English |
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Elsevier
2014-02-07
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Subjects: | UHPLC, Contaminants identification, Environmental samples, Food samples, LTQ-Orbitrap-MS, Pesticide quantification, Ensure availability and sustainable management of water and sanitation for all, |
Online Access: | http://hdl.handle.net/10261/346069 https://api.elsevier.com/content/abstract/scopus_id/84892958135 |
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dig-idaea-es-10261-3460692024-05-14T20:40:26Z Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues Farré, Marinella Picó, Yolanda Barceló, Damià UHPLC Contaminants identification Environmental samples Food samples LTQ-Orbitrap-MS Pesticide quantification Ensure availability and sustainable management of water and sanitation for all The analysis of pesticides residues using a last generation high resolution and high mass accuracy hybrid linear ion trap-Orbitrap mass spectrometer (LTQ-Orbitrap-MS) was explored. Pesticides were extracted from fruits, fish, bees and sediments by QuEChERS and from water by solid-phase with Oasis HLB cartridges. Ultra-high pressure liquid chromatography (UHPLC)-LTQ-Orbitrap mass spectrometer acquired full scan MS data for quantification, and data dependent (dd) MS(2) and MS(3) product ion spectra for identification and/or confirmation. The regression coefficients (r(2)) for the calibration curves (two order of magnitude up to the lowest calibration level) in the study were ≥0.99. The LODs for 54 validated compounds were ≤2ngmL(-1) (analytical standards). The relative standard deviation (RSD), which was used to estimate precision, was always lower than 22%. The recovery of extraction and matrix effects ranged from 58 to 120% and from -92 to 52%, respectively. Mass accuracy was always ≤4ppm, corresponding to a maximum mass error of 1.6millimass units (mmu). This procedure was then successfully applied to pesticide residues in a set of the above-mentioned food and environmental samples. In addition to target analytes, this method enables the simultaneous detection/identification of non-target pesticides, pharmaceuticals, drugs of abuse, mycotoxins, and their metabolites. This work has been supported by the Spanish Ministry of Economy and Competitiveness and by the European Regional Development Fund (ERDF) through the projects “Assessing and Predicting Effects on Water Quantity and Quality in Iberian Rivers Caused by Global Change (SCARCE)” (No. CSD2009-00065, http://www.scarceconsolider.es) and “Evaluation of Emerging Contaminants in the Turia River Basins: From Basic Research to the Application of Environmental Forensics (EMERFOR)” (GCL2011-29703-C02-02, http://mefturia.es). Prof. Barceló acknowledges King Saud University for his visiting professorship. Peer reviewed 2024-02-07T18:08:04Z 2024-02-07T18:08:04Z 2014-02-07 artículo http://purl.org/coar/resource_type/c_6501 Journal of Chromatography A 1328: 66-79 (2014) 00219673 http://hdl.handle.net/10261/346069 10.1016/j.chroma.2013.12.082 24438835 2-s2.0-84892958135 https://api.elsevier.com/content/abstract/scopus_id/84892958135 en Journal of chromatography. A Postprint https://doi.org/10.1016/j.chroma.2013.12.082 Sí open Elsevier |
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UHPLC Contaminants identification Environmental samples Food samples LTQ-Orbitrap-MS Pesticide quantification Ensure availability and sustainable management of water and sanitation for all UHPLC Contaminants identification Environmental samples Food samples LTQ-Orbitrap-MS Pesticide quantification Ensure availability and sustainable management of water and sanitation for all |
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UHPLC Contaminants identification Environmental samples Food samples LTQ-Orbitrap-MS Pesticide quantification Ensure availability and sustainable management of water and sanitation for all UHPLC Contaminants identification Environmental samples Food samples LTQ-Orbitrap-MS Pesticide quantification Ensure availability and sustainable management of water and sanitation for all Farré, Marinella Picó, Yolanda Barceló, Damià Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues |
description |
The analysis of pesticides residues using a last generation high resolution and high mass accuracy hybrid linear ion trap-Orbitrap mass spectrometer (LTQ-Orbitrap-MS) was explored. Pesticides were extracted from fruits, fish, bees and sediments by QuEChERS and from water by solid-phase with Oasis HLB cartridges. Ultra-high pressure liquid chromatography (UHPLC)-LTQ-Orbitrap mass spectrometer acquired full scan MS data for quantification, and data dependent (dd) MS(2) and MS(3) product ion spectra for identification and/or confirmation. The regression coefficients (r(2)) for the calibration curves (two order of magnitude up to the lowest calibration level) in the study were ≥0.99. The LODs for 54 validated compounds were ≤2ngmL(-1) (analytical standards). The relative standard deviation (RSD), which was used to estimate precision, was always lower than 22%. The recovery of extraction and matrix effects ranged from 58 to 120% and from -92 to 52%, respectively. Mass accuracy was always ≤4ppm, corresponding to a maximum mass error of 1.6millimass units (mmu). This procedure was then successfully applied to pesticide residues in a set of the above-mentioned food and environmental samples. In addition to target analytes, this method enables the simultaneous detection/identification of non-target pesticides, pharmaceuticals, drugs of abuse, mycotoxins, and their metabolites. |
format |
artículo |
topic_facet |
UHPLC Contaminants identification Environmental samples Food samples LTQ-Orbitrap-MS Pesticide quantification Ensure availability and sustainable management of water and sanitation for all |
author |
Farré, Marinella Picó, Yolanda Barceló, Damià |
author_facet |
Farré, Marinella Picó, Yolanda Barceló, Damià |
author_sort |
Farré, Marinella |
title |
Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues |
title_short |
Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues |
title_full |
Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues |
title_fullStr |
Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues |
title_full_unstemmed |
Application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues |
title_sort |
application of ultra-high pressure liquid chromatography linear ion-trap orbitrap to qualitative and quantitative assessment of pesticide residues |
publisher |
Elsevier |
publishDate |
2014-02-07 |
url |
http://hdl.handle.net/10261/346069 https://api.elsevier.com/content/abstract/scopus_id/84892958135 |
work_keys_str_mv |
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