Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis
Understanding the potential impact of organic contaminants on male fertility is crucial, yet limited studies have examined these chemicals in semen, with most focusing on urine and blood. To address this gap, we developed and validated a robust LC-HRMS methodology for semen analysis, with a focus on polar and semipolar chemicals. Our methodology enables the quantitative (or semiquantitative) analysis of >2000 chemicals being compatible with suspect and nontarget strategies and providing unprecedented insights into the occurrence and potential bioaccumulation of diverse contaminants in this matrix. We comprehensively analyzed exogenous organic chemicals and associated metabolites in ten semen samples from Spanish participants collected in an area with a large presence of the chemical industry included in the LED-FERTYL Spanish study cohort. This investigation revealed the presence of various contaminants in semen, including plastic additives, PFAS, flame retardants, surfactants, and insecticides. Notably, prevalent plastic additives such as phthalic acid esters and bisphenols were identified, indicating potential health risks. Additionally, we uncovered previously understudied chemicals like the tire additive 2-mercaptobenzothiazole and specific organophosphate flame retardants. This study showcases the potential of our methodology as a valuable tool for large-scale cohort studies, providing insights into the association between contaminant exposure and the risk of male fertility impairments.
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Format: | artículo biblioteca |
Language: | English |
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American Chemical Society
2023-12-05
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Subjects: | Seminal plasma, LED-FERTYL study, Emerging pollutants, High-resolution mass spectrometry (HRMS), Human exposure, Male fertility, Semen quality, http://metadata.un.org/sdg/3, Ensure healthy lives and promote well-being for all at all ages, |
Online Access: | http://hdl.handle.net/10261/340385 https://api.elsevier.com/content/abstract/scopus_id/85178360906 |
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dig-idaea-es-10261-3403852024-05-16T20:36:11Z Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis Sánchez-Resino, Elena Marquès, Montse Gutiérrez-Martín, Daniel Restrepo-Montes, Esteban Martínez, María Ángeles Salas-Huetos, Albert Babio, Nancy Salas-Salvadó, Jordi Gil-Solsona, Rubén Gago-Ferrero, Pablo 0000-0002-1019-7658 0000-0002-4269-1036 0000-0002-5987-0399 Seminal plasma LED-FERTYL study Emerging pollutants High-resolution mass spectrometry (HRMS) Human exposure Male fertility Semen quality http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Understanding the potential impact of organic contaminants on male fertility is crucial, yet limited studies have examined these chemicals in semen, with most focusing on urine and blood. To address this gap, we developed and validated a robust LC-HRMS methodology for semen analysis, with a focus on polar and semipolar chemicals. Our methodology enables the quantitative (or semiquantitative) analysis of >2000 chemicals being compatible with suspect and nontarget strategies and providing unprecedented insights into the occurrence and potential bioaccumulation of diverse contaminants in this matrix. We comprehensively analyzed exogenous organic chemicals and associated metabolites in ten semen samples from Spanish participants collected in an area with a large presence of the chemical industry included in the LED-FERTYL Spanish study cohort. This investigation revealed the presence of various contaminants in semen, including plastic additives, PFAS, flame retardants, surfactants, and insecticides. Notably, prevalent plastic additives such as phthalic acid esters and bisphenols were identified, indicating potential health risks. Additionally, we uncovered previously understudied chemicals like the tire additive 2-mercaptobenzothiazole and specific organophosphate flame retardants. This study showcases the potential of our methodology as a valuable tool for large-scale cohort studies, providing insights into the association between contaminant exposure and the risk of male fertility impairments. The authors acknowledge the efforts made in recruitment and data collection for the LED-FERTYL cohort. This study has been funded by the Instituto de Salud Carlos III (ISCIII) through the project PI21/01447 and co-funded by the European Union. IDAEA-CSIC authors would like to express their gratitude to the Spanish Ministry of Science and Innovation for the support received as “Centro de Excelencia Severo Ochoa 2019–2023”. P.G.-F. acknowledges his Ramón y Cajal fellowship (RYC2019-027913-I) from the AEI-MICI. J.S.-S. is partially supported by ICREA under the ICREA Academia programme. M.Á.M. is supported by the Sara Borrell postdoctoral fellowship [CD21/00045-Instituto de Salud Carlos III (ISCIII)]. E.S.-R is supported by predoctoral fellowship “Martí i Franquès” 2020PMF-PIPF-39. Peer reviewed 2023-12-11T09:37:58Z 2023-12-11T09:37:58Z 2023-12-05 artículo http://purl.org/coar/resource_type/c_6501 Environmental Science and Technology (2023) 0013936X http://hdl.handle.net/10261/340385 10.1021/acs.est.3c04347 37934628 2-s2.0-85178360906 https://api.elsevier.com/content/abstract/scopus_id/85178360906 en Environmental science & technology Postprint https://doi.org/10.1021/acs.est.3c04347 Sí embargo_20241107 American Chemical Society |
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Seminal plasma LED-FERTYL study Emerging pollutants High-resolution mass spectrometry (HRMS) Human exposure Male fertility Semen quality http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Seminal plasma LED-FERTYL study Emerging pollutants High-resolution mass spectrometry (HRMS) Human exposure Male fertility Semen quality http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages |
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Seminal plasma LED-FERTYL study Emerging pollutants High-resolution mass spectrometry (HRMS) Human exposure Male fertility Semen quality http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Seminal plasma LED-FERTYL study Emerging pollutants High-resolution mass spectrometry (HRMS) Human exposure Male fertility Semen quality http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Sánchez-Resino, Elena Marquès, Montse Gutiérrez-Martín, Daniel Restrepo-Montes, Esteban Martínez, María Ángeles Salas-Huetos, Albert Babio, Nancy Salas-Salvadó, Jordi Gil-Solsona, Rubén Gago-Ferrero, Pablo Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis |
description |
Understanding the potential impact of organic contaminants on male fertility is crucial, yet limited studies have examined these chemicals in semen, with most focusing on urine and blood. To address this gap, we developed and validated a robust LC-HRMS methodology for semen analysis, with a focus on polar and semipolar chemicals. Our methodology enables the quantitative (or semiquantitative) analysis of >2000 chemicals being compatible with suspect and nontarget strategies and providing unprecedented insights into the occurrence and potential bioaccumulation of diverse contaminants in this matrix. We comprehensively analyzed exogenous organic chemicals and associated metabolites in ten semen samples from Spanish participants collected in an area with a large presence of the chemical industry included in the LED-FERTYL Spanish study cohort. This investigation revealed the presence of various contaminants in semen, including plastic additives, PFAS, flame retardants, surfactants, and insecticides. Notably, prevalent plastic additives such as phthalic acid esters and bisphenols were identified, indicating potential health risks. Additionally, we uncovered previously understudied chemicals like the tire additive 2-mercaptobenzothiazole and specific organophosphate flame retardants. This study showcases the potential of our methodology as a valuable tool for large-scale cohort studies, providing insights into the association between contaminant exposure and the risk of male fertility impairments. |
author2 |
0000-0002-1019-7658 |
author_facet |
0000-0002-1019-7658 Sánchez-Resino, Elena Marquès, Montse Gutiérrez-Martín, Daniel Restrepo-Montes, Esteban Martínez, María Ángeles Salas-Huetos, Albert Babio, Nancy Salas-Salvadó, Jordi Gil-Solsona, Rubén Gago-Ferrero, Pablo |
format |
artículo |
topic_facet |
Seminal plasma LED-FERTYL study Emerging pollutants High-resolution mass spectrometry (HRMS) Human exposure Male fertility Semen quality http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages |
author |
Sánchez-Resino, Elena Marquès, Montse Gutiérrez-Martín, Daniel Restrepo-Montes, Esteban Martínez, María Ángeles Salas-Huetos, Albert Babio, Nancy Salas-Salvadó, Jordi Gil-Solsona, Rubén Gago-Ferrero, Pablo |
author_sort |
Sánchez-Resino, Elena |
title |
Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis |
title_short |
Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis |
title_full |
Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis |
title_fullStr |
Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis |
title_full_unstemmed |
Exploring the Occurrence of Organic Contaminants in Human Semen through an Innovative LC-HRMS-Based Methodology Suitable for Target and Nontarget Analysis |
title_sort |
exploring the occurrence of organic contaminants in human semen through an innovative lc-hrms-based methodology suitable for target and nontarget analysis |
publisher |
American Chemical Society |
publishDate |
2023-12-05 |
url |
http://hdl.handle.net/10261/340385 https://api.elsevier.com/content/abstract/scopus_id/85178360906 |
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