A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach
This study aims at the application of a marine fungal consortium (Aspergillus sclerotiorum CRM 348 and Cryptococcus laurentii CRM 707) for the bioremediation of diesel oil-contaminated soil under microcosm conditions. The impact of biostimulation (BS) and/or bioaugmentation (BA) treatments on diesel-oil biodegradation, soil quality, and the structure of the microbial community were studied. The use of the fungal consortium together with nutrients (BA/BS) resulted in a TPH (Total Petroleum Hydrocarbon) degradation 42% higher than that obtained by natural attenuation (NA) within 120 days. For the same period, a 72 to 92% removal of short-chain alkanes (C12 to C19) was obtained by BA/BS, while only 3 to 65% removal was achieved by NA. BA/BS also showed high degradation efficiency of long-chain alkanes (C20 to C24) at 120 days, reaching 90 and 92% of degradation of icosane and heneicosane, respectively. In contrast, an increase in the levels of cyclosiloxanes (characterized as bacterial bioemulsifiers and biosurfactants) was observed in the soil treated by the consortium. Conversely, the NA presented a maximum of 37% of degradation of these alkane fractions. The 5-ringed PAH benzo(a)pyrene, was removed significantly better with the BA/BS treatment than with the NA (48 vs. 38 % of biodegradation, respectively). Metabarcoding analysis revealed that BA/BS caused a decrease in the soil microbial diversity with a concomitant increase in the abundance of specific microbial groups, including hydrocarbon-degrading (bacteria and fungi) and also an enhancement in soil microbial activity. Our results highlight the great potential of this consortium for soil treatment after diesel spills, as well as the relevance of the massive sequencing, enzymatic, microbiological and GC-HRMS analyses for a better understanding of diesel bioremediation.
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Springer
2023-08-30
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Subjects: | Marine fungi, Aspergillus sclerotiorum, Bioaugmentation, Biostimulation, Cryptococcus laurentii, Hydrocarbon biodegradation, http://metadata.un.org/sdg/3, Ensure healthy lives and promote well-being for all at all ages, |
Online Access: | http://hdl.handle.net/10261/335001 https://api.elsevier.com/content/abstract/scopus_id/85169566245 |
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dig-idaea-es-10261-3350012024-05-19T20:37:44Z A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach Giovanella, Patricia Taketani, Rodrigo Gouvêa Gil-Solsona, Rubén Saldanha, Luiz Leonardo Naranjo, Samantha Beatríz Esparza Sancho, Juan V. Portolés, Tania Andreote, Fernando Dini Rodríguez-Mozaz, Sara Barceló, Damià Sette, Lara Durães 0000-0002-5980-3786 Marine fungi Aspergillus sclerotiorum Bioaugmentation Biostimulation Cryptococcus laurentii Hydrocarbon biodegradation http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages This study aims at the application of a marine fungal consortium (Aspergillus sclerotiorum CRM 348 and Cryptococcus laurentii CRM 707) for the bioremediation of diesel oil-contaminated soil under microcosm conditions. The impact of biostimulation (BS) and/or bioaugmentation (BA) treatments on diesel-oil biodegradation, soil quality, and the structure of the microbial community were studied. The use of the fungal consortium together with nutrients (BA/BS) resulted in a TPH (Total Petroleum Hydrocarbon) degradation 42% higher than that obtained by natural attenuation (NA) within 120 days. For the same period, a 72 to 92% removal of short-chain alkanes (C12 to C19) was obtained by BA/BS, while only 3 to 65% removal was achieved by NA. BA/BS also showed high degradation efficiency of long-chain alkanes (C20 to C24) at 120 days, reaching 90 and 92% of degradation of icosane and heneicosane, respectively. In contrast, an increase in the levels of cyclosiloxanes (characterized as bacterial bioemulsifiers and biosurfactants) was observed in the soil treated by the consortium. Conversely, the NA presented a maximum of 37% of degradation of these alkane fractions. The 5-ringed PAH benzo(a)pyrene, was removed significantly better with the BA/BS treatment than with the NA (48 vs. 38 % of biodegradation, respectively). Metabarcoding analysis revealed that BA/BS caused a decrease in the soil microbial diversity with a concomitant increase in the abundance of specific microbial groups, including hydrocarbon-degrading (bacteria and fungi) and also an enhancement in soil microbial activity. Our results highlight the great potential of this consortium for soil treatment after diesel spills, as well as the relevance of the massive sequencing, enzymatic, microbiological and GC-HRMS analyses for a better understanding of diesel bioremediation. This research was financially supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) (grant #2016/07957-7) and by Conselho Nacional de Desenvolvimento Tecnológico (CNPq) (grant #407986/2018-6). Peer reviewed 2023-09-13T08:15:28Z 2023-09-13T08:15:28Z 2023-08-30 artículo http://purl.org/coar/resource_type/c_6501 Environmental Science and Pollution Research (2023) 09441344 http://hdl.handle.net/10261/335001 10.1007/s11356-023-29474-w 37648922 2-s2.0-85169566245 https://api.elsevier.com/content/abstract/scopus_id/85169566245 en Environmental science and pollution research international Postprint https://doi.org/10.1007/s11356-023-29474-w Sí embargo_20240830 Springer |
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Marine fungi Aspergillus sclerotiorum Bioaugmentation Biostimulation Cryptococcus laurentii Hydrocarbon biodegradation http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Marine fungi Aspergillus sclerotiorum Bioaugmentation Biostimulation Cryptococcus laurentii Hydrocarbon biodegradation http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages |
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Marine fungi Aspergillus sclerotiorum Bioaugmentation Biostimulation Cryptococcus laurentii Hydrocarbon biodegradation http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Marine fungi Aspergillus sclerotiorum Bioaugmentation Biostimulation Cryptococcus laurentii Hydrocarbon biodegradation http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages Giovanella, Patricia Taketani, Rodrigo Gouvêa Gil-Solsona, Rubén Saldanha, Luiz Leonardo Naranjo, Samantha Beatríz Esparza Sancho, Juan V. Portolés, Tania Andreote, Fernando Dini Rodríguez-Mozaz, Sara Barceló, Damià Sette, Lara Durães A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach |
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This study aims at the application of a marine fungal consortium (Aspergillus sclerotiorum CRM 348 and Cryptococcus laurentii CRM 707) for the bioremediation of diesel oil-contaminated soil under microcosm conditions. The impact of biostimulation (BS) and/or bioaugmentation (BA) treatments on diesel-oil biodegradation, soil quality, and the structure of the microbial community were studied. The use of the fungal consortium together with nutrients (BA/BS) resulted in a TPH (Total Petroleum Hydrocarbon) degradation 42% higher than that obtained by natural attenuation (NA) within 120 days. For the same period, a 72 to 92% removal of short-chain alkanes (C12 to C19) was obtained by BA/BS, while only 3 to 65% removal was achieved by NA. BA/BS also showed high degradation efficiency of long-chain alkanes (C20 to C24) at 120 days, reaching 90 and 92% of degradation of icosane and heneicosane, respectively. In contrast, an increase in the levels of cyclosiloxanes (characterized as bacterial bioemulsifiers and biosurfactants) was observed in the soil treated by the consortium. Conversely, the NA presented a maximum of 37% of degradation of these alkane fractions. The 5-ringed PAH benzo(a)pyrene, was removed significantly better with the BA/BS treatment than with the NA (48 vs. 38 % of biodegradation, respectively). Metabarcoding analysis revealed that BA/BS caused a decrease in the soil microbial diversity with a concomitant increase in the abundance of specific microbial groups, including hydrocarbon-degrading (bacteria and fungi) and also an enhancement in soil microbial activity. Our results highlight the great potential of this consortium for soil treatment after diesel spills, as well as the relevance of the massive sequencing, enzymatic, microbiological and GC-HRMS analyses for a better understanding of diesel bioremediation. |
author2 |
0000-0002-5980-3786 |
author_facet |
0000-0002-5980-3786 Giovanella, Patricia Taketani, Rodrigo Gouvêa Gil-Solsona, Rubén Saldanha, Luiz Leonardo Naranjo, Samantha Beatríz Esparza Sancho, Juan V. Portolés, Tania Andreote, Fernando Dini Rodríguez-Mozaz, Sara Barceló, Damià Sette, Lara Durães |
format |
artículo |
topic_facet |
Marine fungi Aspergillus sclerotiorum Bioaugmentation Biostimulation Cryptococcus laurentii Hydrocarbon biodegradation http://metadata.un.org/sdg/3 Ensure healthy lives and promote well-being for all at all ages |
author |
Giovanella, Patricia Taketani, Rodrigo Gouvêa Gil-Solsona, Rubén Saldanha, Luiz Leonardo Naranjo, Samantha Beatríz Esparza Sancho, Juan V. Portolés, Tania Andreote, Fernando Dini Rodríguez-Mozaz, Sara Barceló, Damià Sette, Lara Durães |
author_sort |
Giovanella, Patricia |
title |
A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach |
title_short |
A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach |
title_full |
A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach |
title_fullStr |
A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach |
title_full_unstemmed |
A comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach |
title_sort |
comprehensive study on diesel oil bioremediation under microcosm conditions using a combined microbiological, enzymatic, mass spectrometry, and metabarcoding approach |
publisher |
Springer |
publishDate |
2023-08-30 |
url |
http://hdl.handle.net/10261/335001 https://api.elsevier.com/content/abstract/scopus_id/85169566245 |
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