Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants
Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals).
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Format: | artículo biblioteca |
Language: | English |
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Elsevier
2023-01-01
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Subjects: | Suspect screening, Bead beating, Human biomonitoring, Human tissues, LC-HRMS, Non-target screening, |
Online Access: | http://hdl.handle.net/10261/296163 https://api.elsevier.com/content/abstract/scopus_id/85148075900 |
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dig-idaea-es-10261-2961632024-05-17T21:21:05Z Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants Gutiérrez-Martín, Daniel Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén 0000-0002-4269-1036 Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals). This work was supported by Ramón y Cajal fellowship (RYC2019–027913-I, Pablo Gago-Ferrero) from the AEI-MICI. Authors also acknowledge the Spanish Ministry of Science and Innovation through the support received as “Centro de Excelencia Severo Ochoa 2019–2023″. This study is partially supported by a grant from Instituto de Salud Carlos III (ISCIII) (PI20/00360 to AP-E and CM) with support of Bellvitge Biomedical Research Institute (IDIBELL). Co-funded by European Regional Development Fund, ERDF, a way to build Europe. We thank CERCA program / Generalitat de Catalunya for Institutional Support. Peer reviewed 2023-02-28T11:09:20Z 2023-02-28T11:09:20Z 2023-01-01 artículo http://purl.org/coar/resource_type/c_6501 MethodsX 10: 102069 (2023) http://hdl.handle.net/10261/296163 10.1016/j.mex.2023.102069 2-s2.0-85148075900 https://api.elsevier.com/content/abstract/scopus_id/85148075900 en MethodsX Publisher's version https://doi.org/10.1016/j.mex.2023.102069 Sí open Elsevier |
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Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening |
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Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening Gutiérrez-Martín, Daniel Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
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Little is known about the presence of organic pollutants in human brain (and even less in brain tumors). In this regard, it is necessary to develop new analytical protocols capable of identifying a wide range of exogenous chemicals in this type of samples (by combining target, suspect and non-target strategies). These methodologies should be robust and simple. This is particularly challenging for solid samples, as reliable extraction and clean-up techniques should be combined to obtain an optimal result. Hence, the present study focuses on the development of an analytical methodology that allows the screening of a wide range of organic chemicals in brain and brain tumor samples. This protocol was based on a solid-liquid extraction based on bead beating, solid-phase extraction clean-up with multi-layer mixed-mode cartridges, reconstitution and LC–HRMS analysis. To evaluate the performance of the extraction methodology, a set of 66 chemicals (e.g., pharmaceuticals, biocides, or plasticizers, among others) with a wide range of physicochemical properties was employed. Quality control parameters (i.e., linear range, sensitivity, matrix effect (ME%), and recoveries (R%)) were calculated and satisfactory results were obtained for them (e.g., R% within 60–120% for 32 chemicals, or ME% higher than 50% (signal suppression) for 79% of the chemicals). |
author2 |
0000-0002-4269-1036 |
author_facet |
0000-0002-4269-1036 Gutiérrez-Martín, Daniel Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén |
format |
artículo |
topic_facet |
Suspect screening Bead beating Human biomonitoring Human tissues LC-HRMS Non-target screening |
author |
Gutiérrez-Martín, Daniel Marquès, Montse Pons-Escoda, Albert Vidal, Noemi Bruna, Jordi Restrepo-Montes, Esteban López-Serna, Rebeca García-Sayago, Francisco Majos, Carles Gago-Ferrero, Pablo Gil-Solsona, Rubén |
author_sort |
Gutiérrez-Martín, Daniel |
title |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
title_short |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
title_full |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
title_fullStr |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
title_full_unstemmed |
Tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
title_sort |
tumoral and normal brain tissue extraction protocol for wide-scope screening of organic pollutants |
publisher |
Elsevier |
publishDate |
2023-01-01 |
url |
http://hdl.handle.net/10261/296163 https://api.elsevier.com/content/abstract/scopus_id/85148075900 |
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